Protective Effects of Methane-Rich Saline on Renal Ischemic-Reperfusion Injury in a Mouse Model.

BACKGROUND Renal ischemic-reperfusion (RIR) injury remains a major cause of acute kidney injury, with increased in-hospital mortality and risks for chronic kidney disease. Previous studies have proposed that oxidative stress, inflammation, and renal apoptosis are the most common causes of injury, whereas recent research proved that methane, the simplest alkane generated by an enteric microorganism or accompanying the production of reactive oxygen species (ROS), can alleviate inflammation and oxidative stress and reduce apoptosis in different organs. MATERIAL AND METHODS In the present study, we analyzed the possible effects of methane-rich saline in RIR injury in a mouse model and analyzed its possible protective effects on inflammation, oxidative stress, and apoptosis.

A novel ATP7B gene mutation in a liver failure patient with normal ceruloplasmin and low serum alkaline phosphatase.

Wilson's disease (WD) is a rare disorder of copper metabolism resulting in accumulation of copper in liver and other organs. We present a liver failure patient, who was misdiagnosed for two years, with normal ceruloplasmin and low serum alkaline phosphatase. Molecular testing revealed a novel p.

[(1)H-MRS study of auditory cortex in patients with presbycusis].

Objective: To study the metabolic changes of auditory cortex in patients with presbycusis by using proton magnetic resonance spectroscopy ((1)H-MRS).

Methods: Ten normal hearing volunteers (youth group), 10 normal hearing of elderly (aged group) and 8 patients with presbycusis (presbycusis group) were checked with proton magnetic resonance spectroscopy. N-acetylaspartic acid (NAA), creatine (Cr), choline (Cho), γ-aminobutyric acid (GABA), glutamic acid (Glu) compound were measured.

[Hand-assisted laparoscopic hepatectomy for large liver cancer in 56 cases].

Objective: To investigate the practical value of hand-assisted laparoscopic hepatectomy for large liver cancer in peripheral segments.

Methods: From March 2004 to December 2007, 56 patients with large liver cancer underwent hand-assisted laparoscopic hepatectomy including 53 cases of hepatocellular carcinoma, 2 cases of cholangiocellular carcinoma, 1 case of hepatic metastatic squamous carcinoma.

Results: The operation procedures were completed safely in all patients including 27 left lateral segment hepatectomy, 6 left hemi-hepatectomy and 23 atypical right hepatectomy.

[Application of reversed pedicled submental island flap].

Objective: To explore the clinical applications of reversed pedicled submental island flap in the face and oropharynx.

Methods: The clinical data of ten cases of reconstruction of defect in the area of face or oropharynx following resection of tumors with reversed pedicled submental island flaps between January of 2004 to December of 2006 were retrospectively studied. The cases included six males and four females, aged from 24 to 76 (median 55 years).

[Study on p53 tetramerization domain in improving functional affinity and biological activity of antibody].

Objective: To fuse the genes of p53 tetramerization domain and anti-CD3/anti-prostate-cancer bispecific single-chain antibody (BsAb), and exploit a new way to improve the functional affinity and biological activity of antibody.

Methods: Genes of p53 tetramerization domain and anti-CD3/anti-prostate-cancer BsAb was fused by technique of DNA sub-cloning. The fusion gene confirmed by sequencing was subcloned into the pSectag2-B plasmid.

[Construction and expression of anti-CD3/anti-prostate-cancer bispecific single-chain antibody].

Objective: To construct a recombinant vector that expresses anti-CD 3/anti-prostate-cancer bispecific single-chain antibody (BsAb), and study its biological activities and clinical significance.

Methods: An anti-CD 3/anti-prostate-cancer BsAb was constructed by PCR and molecular biological technique of DNA cloning. The fusion gene, confirmed by sequencing, was subcloned into the pSectag2-B plasmid from the pUC18 vector by digestion with EcoR I and Hind III restriction endonucleases, whose sites exist in both the vectors.

[HLA-amino acid residue matching in organ transplantation].

Objective: To present a new matching policy-HLA amino acid residue matching (Res M) for Chinese population.

Methods: Based on the outcome of genotyping for HLA alleles in massive samples, we presented new Res M according to 10 groups of amino acid residue for class I and, groups of DR/DQ for class II and compared them with HLA six-antigen match program (Ag M). The influence of Res M on early renal function, acute rejection and 1-5 year graft survivals were retrospectively analyzed in 316 cases of first cadaver kidney transplantation.