[Research on apoptosis changes of tensor veli palatini in patients with obstructive sleep apnea-hypopnea syndrome].

Objective: To explore apoptosis changes of dilator muscles in the upper airway by detecting the expression of Bax, Bcl-2 in tensor veli palatini in patients with OSAHS.

Method: The expression of Bax and Bcl-2 were detected in tensor veli palatini in 30 cases with OSAHS and 10 cases chronic tonsillitis without OSAHS by immunohistochemistry and image analytical system, and the results were analyzed.

Result: (1) The expression levels of Bax in the OSAHS group increased significantly compared to control group (P < 0.

[Prenatal molecular diagnosis of four fetuses at high risk for X-linked adrenoleukodystrophy].

Objective: To investigate methods for prenatal molecular diagnosis of fetuses at high risk for X-linked adrenoleukodystrophy (X-ALD).

Methods: The amniotic fluid was obtained and genomic DNA was isolated from amniotic fluid cells. Maternal contamination was evaluated by paternity test.

Anatomic basis of perforator flaps of medial vastus muscle.

The purpose of this study was to elucidate anatomical features of perforating branch flaps based on the muscular branches of the medial vastus muscle and to seek a new, applicable technique that could be used in repairing soft tissue defects around human knees. In this study, the origin, the course, the branches, the distribution, and the distal anastomosis of the muscular branch of the medial vastus muscle were observed in 30 sides of adult cadaveric lower limb specimens with the adductor tubercle, the patella midpoint, and the inguinal ligament midpoint as the observation markers. The specimens had been perfused arterially with red gelatin before they were supplied.

Preoperative single-bolus high-dose antithymocyte globulin as induction therapy in sensitized renal transplant recipients.

Background: Immunological sensitization remains a major problem following renal transplantation. There is no consensus for the management of sensitized renal allograft recipients. The patients become tethered to dialysis while waiting for compatible donors.

Anatomic study of distally based pedicle compound flaps with nutrient vessels of the cutaneous nerves and superficial veins of the forearm.

The purpose of this study was to describe the anatomic basis for a distally based neurovenovascular pedicle compound flap, with nutrient vessels of the cutaneous nerves and superficial veins of the forearm. In this study, the origins, branches, and anastomoses of nutrient vessels of the cutaneous nerves and superficial veins of the forearm and their relationships with the blood supply of adjacent muscle, bone, and skin were assessed in 96 adult cadavers by perfusion of red gelatin into the superior limb arteries. The results showed that the nutrient vessels of cutaneous nerves and superficial veins of the forearm were found to have multiple origins, consisting of six longitudinal vascular plexuses and one transverse vascular plexus of the forearm, as follows: 1) the anterior-lateral vascular plexus from cutaneous branches of the radial artery; 2) the anterior-medialis vascular plexus from cutaneous branches of the ulnar artery; 3) the dorso-lateral vascular plexus from radial osteal and cutaneous branches; 4) the dorso-medialis vascular plexus from ulnar osteal and cutaneous branches; 5) the radial vascular plexus from osteal and cutaneous branches of the radial artery, cutaneous branches of the radial artery in the upper wrist, recurrent branches of the styloid process of the radius, and the radialis vascular plexus of cutaneous branches of the tabatière anatomique (anatomical snuffbox); and 6) the ulnar lateral vascular plexus from cutaneous branches of the ulnar artery in the upper wrist and osteal and cutaneous branches.

[Human bone marrow multipotent adult progenitor cells differentiate into hepatocyte-like cells with hepatocyte growth factor plus fibroblast growth factor-4 in vitro].

Objective: To investigate the possibility of the human bone marrow multipotent adult progenitor cells (hMAPCs) to differentiate into hepatocytes with hepatocyte growth factor (HGF)/ fibroblast growth factor-4 (FGF-4) in vitro.

Methods: (1) Obtaining the hMAPCs. Bone marrow was obtained from volunteers and then centrifuged through density gradient centrifugation methods.

[Multivariate analysis of the parameters related to recurrence of laryngeal papilloma in adult].

Objective: To investigate the clinical and pathological characteristics of astrocytoma and the factors related to pathological gradings as well as recurrence in laryngeal papilloma in adult.

Method: Fifty samples from patients were selected and 10 cases of normal tissue or inflammation tissue were used as control group. The expression of Pgp, GST-pi, Topo II and Ki-67 and VEGF by immunohistochemical stain and the microvessel density by morphometry were studied.

[Animal model building of hepaticojejunostomy and hepaticocholedochostomy and comparison of short-term effect].

Objective: To discuss the way of animal model building of hepaticocholedochostomy (HC) and hepaticojejunostomy (HJ) and to compare the short-term effect.

Methods: Twenty-nine dogs were divided randomly into control group (n=5) and the experimental group (stenosis of left hepatic duct, n=24). After 7 weeks of stenosis of left hepatic duct, 24 dogs in the experimental group were divided randomly into HC subgroup (n=12) and HJ subgroup (n=12).

[Effect of Puerarin on platelet activating factors CD63 and CD62P, plasminogen activator inhibitor and C-reactive protein in patients with unstable angia pectoris].

Objective: To explore the action of platelet surface activity protein (platelet granule membrane protein CD63 and lysosome membrane protein CD62P), plasminogen activator inhibitor (PAI-1) and C-reactive protein (C-RP) in occurrence and development of unstable angina pectoris (UAP), and the effect of Puerarin on them.

Methods: Patients with UAP were randomly divided into the treated group (32 cases, treated with Puerarin) and the control group (27 cases, treated with Ticlid), the therapeutic course was 4 weeks for both groups. Changes of CD63, CD62P, PAI-1 and C-RP before and after treatment were observed.

[Renal protective effects of vitamin E in diabetic rats].

Objective: To investigate the renal protective effect of vitamin E on streptozotocin induced diabetic rats, as well as the possible mechanism involved in.

Methods: Vitamin E 20 mg x kg(-1) x d(-1) was given to diabetic rats for 8 weeks. Urinary albumin excretion (UAE) was measured by radioimmunoassay.

[Clinicopathologic criteria for the diagnosis of extranodal anaplastic large-cell lymphoma: a preliminary study].

Objective: To study and observe the clinicopathologic features of extranodal Ki-1 (CD30) positive anaplastic large-cell lymphoma (ALCL) in order to assess its diagnostic criteria.

Methods: Lymph nodes of 12 patients with Ki-1 positive ALCL were examined histopathologically and immunohistochemically.

Results: There were distinctive histopathological features of ALCL cells.

[Dose distributions of linac-based radiosurgery for pineal region mass].

The doses of linac convergent-beam irradiations to pineal region were measured in an anthropomorphic phantom. The feasibility of a linac accelerator as a radiosurgery tool to treat small sized intracranial masses was evaluated. The influences of some irradiation parameters on the dose distributions were discussed.

[Role of nitric oxide in pathogenesis of pregnancy induced hypertension].

Objective: To investigate the role of nitric oxide (NO) in pathogenesis of pregnancy induced hypertension (PIH).

Methods: N(G)-nitro-L-arginine-methyl-ester (L-NAME) was used to inhibite the synthesizing of NO in the third trimester of pregnancy rats. Indices including HGB, PLT, HCT, protein uria, serum endothelin (ET), thromboxane B2(TXB2), 6-keto-PGF1 alpha and blood pressure in pregnant rats were measured.

[The immunophenotypic lymphocyte analysis of orbital lymphadenosis and its significance].

An immunophenotypic lymphocyte analysis of 56 cases of orbital lymphadenosis was performed with a panel of 10 monoclonal antibodies against the lymphocyte differentiation antigen by the streptavidin-peroxidase conjugate method to reveal that (1) all 15 cases of inflammatory pseudotumor and 32 of 33 cases of reactive lymphocyte hyperplasia manifested polyclonality of the constituent cells; (2) the immunophenotype of cells was monoclonal in 2 of 3 cases of atypical lymphocyte hyperplasia and diagnosis of malignant lymphoma (ML) were established; and (3) among 5 cases of ML, the cells were shown to be B-cells in 4 cases and T-cells in 1 case. These results suggested that immunophenotyping could be useful in differentiating the benign from the malignant orbital lymphadenosis and the histologic types of ML. The relationship between pathologic and immunologic classifications of orbital lymphadenosis was also discussed.