Efficient production of phenyllactic acid in via metabolic engineering and fermentation optimization strategies.

Phenyllactic acid (PhLA), an important natural organic acid, can be used as a biopreservative, monomer of the novel polymeric material poly (phenyllactic acid), and raw material for various medicines. Herein, we achieved a high-level production of PhLA in through the application of metabolic engineering and fermentation optimization strategies. First, the PhLA biosynthetic pathway was established in CGSC4510, and the phenylalanine biosynthetic pathway was disrupted to improve the carbon flux toward PhLA biosynthesis.

Polygonatum sibiricum component liquiritigenin restrains breast cancer cell invasion and migration by inhibiting HSP90 and chaperone-mediated autophagy.

Breast cancer (BC) is most commonly diagnosed worldwide. Liquiritigenin is a flavonoid found in various species of the Glycyrrhiza genus, showing anti-tumor activity. This article was to explore the influences of liquiritigenin on the biological behaviors of BC cells and its underlying mechanism.

 (Mazaceae), a new species from China: evidence from morphological and molecular analyses.

Utilising both morphological and molecular analyses, this study unveils , a novel addition to the Mazaceae family, discovered in Shaowu County, Fujian Province, China. The comprehensive description and illustrations provided here are a result of a meticulous exploration of its morphological features. While bearing a resemblance to , this new-found species is distinguished by three distinct characteristics: its stems are delicately soft, its leaves possess a membranous quality and the ovary is notably villous at the apex.

Afferent Renal Denervation Attenuates Sympathetic Overactivation From the Paraventricular Nucleus in Spontaneously Hypertensive Rats.

Background: The effectiveness of renal denervation (RDN) in reducing blood pressure and systemic sympathetic activity in hypertensive patients has been established. However, the underlying central mechanism remains unknown. This study aimed to investigate the role of RDN in regulating cardiovascular function via the central renin-angiotensin system (RAS) pathway.

Effects of oregano essential oil Pickering emulsion and ZnO nanoparticles on the properties and antibacterial activity of konjac glucomannan/carboxymethyl chitosan nanocomposite films.

Green and environmentally friendly natural bio-based food packaging films are increasingly favored by consumers. This study incorporated carboxylated-cellulose nanocrystal stabilized oregano essential oil (OEO) Pickering emulsion and ZnO nanoparticles (ZNPs) into konjac glucomannan (KGM)/carboxymethyl chitosan (CMCS) complexes to develop active food packaging films. The effects of OEO Pickering emulsion and ZNPs on the physical, structural, and antimicrobial activities of the nanocomposite films were evaluated.

FvKex2 is required for development, virulence, and mycotoxin production in Fusarium verticillioides.

Fusarium verticillioides is one of the most important fungal pathogens causing maize ear and stalk rots, thereby undermining global food security. Infected seeds are usually unhealthy for consumption due to contamination with fumonisin B1 (FB1) mycotoxin produced by the fungus as a virulence factor. Unveiling the molecular factors that determine fungal development and pathogenesis will help in the control and management of the diseases.

Influence of cultivation substrate on antioxidant activities and triterpenoid profiles of the fruiting body of .

Introduction: The fruiting body of Ganoderma lucidum has been believed to possess a wide range of therapeutic effects. There are two main methods for artificial cultivation of G. lucidum to produce the fruiting body, namely wood log cultivation and substitute cultivation.

High -Sensitive Detection of Malachite Green Based on Surface-Enhanced Electrochemiluminescence.

This article introduces a novel unlabeled surface-enhanced electrochemiluminescence (SEECL) sensor for malachite green (MG) detection. The SEECL sensor was prepared by modifying the Ru(bpy) doped gold-SiO core-shell nanocomposites (Au@SiO-Ru(bpy)) on the gold electrode. Ru(bpy) of nanocomposites can not only emit electrochemiluminescence (ECL) with electrochemical reaction, but also induce the local surface plasmon resonance (LSPR) of gold core.

Design of cationic surfactant reinforced carrageenan waterproof composite films and applied as water induced electricity generator.

Carrageenan (CR) is a renewable polysaccharide material for packaging application due to its good film-forming property, but its use can be limited by the water solubility. In this research, CR hydrogels were modified by quaternary ammonium surfactants with different length of hydrocarbon tails (n, 8≦n≦16) by adsorption method and waterproof films were obtained after drying. The composition and charge interaction of composite films was confirmed by FTIR.

The impact of resilience on clinical nurses' moral courage during COVID-19: A moderated mediation model of ethical climate and moral distress.

Aim: The purpose of this study was to explore whether clinical ethical climate mediates the relationship between resilience and moral courage in a population of clinical nurses during COVID-19, and if moral distress faced by nurses is a moderating factor.

Background: Resilience can help nurses maintain their personal health during COVID-19 when they face great physical and psychological shock and are prone to health problems. Moral courage, as an ethical competency, helps nursing staff in adhering to the principles and values of professional ethics.

Clip-SP1 cleavage activates downstream prophenoloxidase activating protease (PAP) in Plutella xylostella.

Melanization is a component of the humoral immune defense of insects and is induced by serine protease-mediated phenoloxidase (PO) catalysis. Prophenoloxidase (PPO) in the midgut of Plutella xylostella is activated by the CLIP domain serine protease (clip-SP) in response to Bacillus thuringiensis (Bt) infection, but the detailed signaling cascade following this activation is unknown. Here, we report that activation of clip-SP enhances PO activity in the P.

Multi-omics in Spinal Cord Injury: Diagnosis, Prognosis, and Treatment.

A spinal cord injury (SCI) can result in varying degrees of limb movement impairment and sensory impairment, affecting the quality of life severely. The study of the molecular mechanisms underlying SCI disease has advanced greatly. However, there is still room for improvement in terms of the cognitive and systematic approaches used for disease diagnosis, progression, treatment, and prognosis.

Novel covalent organic frameworks based electrospun composite nanofiber membranes as pipette-tip strong anion exchange sorbent for determination of inorganic arsenic in rice.

Novel covalent organic frameworks (COFs) based PAN@TpBD(NH) electrospun composite nanofiber membranes (ECNMs) were fabricated as strong anion exchange sorbent by implementing electrospinning technology. The finished sorbent was characterized, and key parameters of pipette-tip solid phase extraction (PTSPE) procedures were investigated. Inorganic arsenic (iAs) was successfully separated from rice under the optimal precondition conditions, and quantified by hydride generation-atomic fluorescence spectrometry (HG-AFS).

Transcriptome Analysis Explored the Differential Genes' Expression During the Development of the Fruiting Body.

(. ) is a fungus with great edible and nutritional values; however, the development mechanism of its fruiting body has not been studied. Thus, this study aimed to analyze the differentially expressed genes (DEGs) in four stages; primordia stage (Sra1), young mushroom stage (Sra2), picking stage (Sra3), and opening umbrella stage (Sra4).

UHPLC-Q-Orbitrap-MS-Based Metabolomics Reveals Chemical Variations of Two Types of Rhizomes of .

The rhizomes of are commonly consumed as food and also used as medicine. However, the metabolic profile of has not been fully revealed yet. Recently, we developed a novel evergreen species of P.

Influence of selenium on the mycelia of the shaggy bracket fungus, Inonotus hispidus.

Background: Selenium (Se) is a needed trace element for animals and humans. Many fungi have effective mechanisms to acquire, transform and accumulate Se in organic form. In this study, the effects of inorganic Se (sodium selenite) on the medicinal fungus Inonotus hispidus was investigated.

Complete plastid genome of (Orchidaceae, Aeridinae) and phylogenetic analysis.

The complete plastid genome of was determined and analyzed in this work. The plastome was 146,897 bp in length with 83,366 bp of the large single-copy (LSC) region, 11,957 bp of the small single-copy (SSC) region, and 25,787 bp of the invert repeats (IR) regions. The genome contained 127 genes, 74 protein-coding genes, 38 tRNA genes, and 8 rRNA genes.

Nuciferine reduced fat deposition by controlling triglyceride and cholesterol concentration in broiler chickens.

The purpose of this study was to investigate whether dietary nuciferine affects lipid metabolism in broiler chickens. Four treatment groups were made from 120 1-day-old broiler chickens including the base diet group (normal control [NC], supplemented with 0 mg/kg of nuciferine) and groups treated with 25 mg/kg, 100 mg/kg, and 400 mg/kg of dietary nuciferine, which was supplemented for 42 d. The results showed that body weight, average daily weight gain, and absolute and relative fat and liver weight were significantly decreased with nuciferine supplementation.

Determination of copper ions in herbal medicine based on click chemistry using an electronic balance as a readout.

The amount of copper affects the quality of herbal medicine greatly, it is necessary to develop some simple and sensitive methods to detect copper for the remote or resource-limited area. An electronic balance is one of the most familiar equipment that can be found nearly in all laboratories. The presence of Cu(i) can catalyze azide-alkyne cycloaddition reaction (called as click chemistry) with high efficiency.

Characterization of Gut Bacteria and Their Role in Feeding and Growth of the Host.

Insect gut microbes play important roles in host feeding, digestion, immunity, growth and development. is an important agricultural pest distributed of global importance. In the present study, diversity and functions of the gut bacteria in are investigated based on the approaches of metagenomics and denaturing gradient gel electrophoresis (DGGE).

Triptolide alleviates radiation-induced pulmonary fibrosis via inhibiting IKKβ stimulated LOX production.

Lysyl oxidase (LOX) is involved in fibrosis by catalyzing collagen cross-linking. Previous work observed that Triptolide (TPL) alleviated radiation-induced pulmonary fibrosis (RIPF), but it is unknown whether the anti-RIPF effect of TPL is related to LOX. In a mouse model of RIPF, we found that LOX persistently increased in RIPF which was significantly lowered by TPL.

Effect of VIB Gene on Cellulase Production of Trichoderma orientalis EU7-22.

The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor.

Immune responses to Bacillus thuringiensis in the midgut of the diamondback moth, Plutella xylostella.

The diamondback moth, Plutella xylostella, is the first insect to develop resistance to Bacillus thuringiensis (Bt) in the field. To date, little is known about the molecular mechanism of the interaction between Bt and midgut immunity in P. xylostella.

A surface-enhanced electrochemiluminescence sensor based on Au-SiO core-shell nanocomposites doped with Ru(bpy) for the ultrasensitive detection of prostate-specific antigen in human serum.

This study reports a surface-enhanced electrochemiluminescence (SEECL) sensor for the ultrasensitive detection of prostate-specific antigen (PSA) in human serum. First, we prepared the Au-SiO2 core-shell nanocomposites doped with Ru(bpy)32+ (Au@SiO2-Ru) as the ECL signal generation and amplification element of the SEECL sensor. Ru(bpy)32+ could emit ECL under the excitation of an electrochemical reaction, and generate the local surface plasmon resonance (LSPR) electromagnetic field from the gold nanoparticle (AuNP) core.