Development of a Dual-Readout Multicolor Immunoassay for the Rapid Analysis of Isocarbophos in Vegetable and Fruit Samples.

Multicolor immunoassay is a powerful tool for rapid analysis without the use of bulky instruments owing to various color conversions, which is suitable for on-site visual analysis for pesticides. Herein, this study developed a multicolor immunoassay for the rapid detection of isocarbophos. After competitive immunoassay, the secondary antibody (GAM-ALP) catalyzed ascorbyl-2-phosphate (AAP) into ascorbic acid (AA).

White Tea Aqueous Extract: A Potential Anti-Aging Agent Against High-Fat Diet-Induced Senescence in .

White tea has been scientifically proven to exhibit positive biological effects in combating chronic diseases, including cancer, metabolic syndrome, etc. Nevertheless, the anti-aging activity and mechanism of white tea on organisms exposed to a high-fat diet remain unexplored. Herein, we prepared a white tea aqueous extract (WTAE) from white peony in Fuding and assessed its in vivo antioxidant and anti-aging effects by employing a senescence model induced by lard, delving into the underlying molecular mechanisms through which the WTAE contributes to lifespan improvement.

Mitigating Oxidative Stress and Promoting Cellular Longevity with Mushroom Extracts.

Oxidative stress can disrupt the body's ability to fight harmful free radicals, leading to premature aging and various health complications. This study investigated the antioxidant and anti-aging properties of four medicinal and edible mushrooms: , , , and . The antioxidant activity of mushroom extracts was evaluated using (DPPH-ABTS-Reducing power).

Determination of Amino Acids of Novel Food in Food by HPLC Coupled with Pre-Column Derivatization.

A method for simultaneous determination of Theanine and γ-aminobutyric acid (GABA), as the novel food of amino acids, which was established using pre-column derivatization and high-performance liquid chromatography (HPLC). 4-Dimethylaminoazobe nzene-4'-sulfonyl chloride (DABS-Cl) is employed as the derivatization reagent with chromophore linked to Theanine and GABA, which lacks chromophore for DAD analysis in its pristine structure. After the detection wavelength was confirmed, the chromatographic and derivatization conditions were also optimized, including the chromatographic column, mobile phases and their gradient, derivatization temperature and time, the additive amount of buffer solution and derivatization reagent.

Identification and Expression Analysis of Gene in Bitter Gourd ().

Background: Lipoxygenases (LOXs) are key enzymes in the unsaturated fatty acid oxidation reaction pathway and play an important regulatory role in the synthesis of fruit aroma volatiles.

Methods: gene family members were identified in the whole genome database of bitter gourd and analyzed bioinformatically. An RT-qPCR was used to analyze the expression differences in different tissues.

Overexpression Enhances Anthocyanin Biosynthesis, Modulates the Composition Ratio, and Increases Antioxidant Activity in Cells.

Anthocyanins are significant secondary metabolites that are essential for plant growth and development, possessing properties such as antioxidant, anti-inflammatory, and anti-cancer activities and cardiovascular protection. They offer significant potential for applications in food, medicine, and cosmetics. However, since anthocyanins are mainly obtained through plant extraction and chemical synthesis, they encounter various challenges, including resource depletion, ecological harm, environmental pollution, and the risk of toxic residuals.

Function Analysis of Heme Peroxidase Genes, MpPxd2 and MpPxd4, Under Thiacloprid Exposure in the Neonicotinoid-Resistant (Sulzer).

The green peach aphid, , is a notorious pest worldwide. We collected a field population of the pest (FZQ-F) that exhibited high resistance to neonicotinoids. Exposure to neonicotinoids can induce oxidative damage in animals; however, it remains unclear whether antioxidant enzymes contribute to the innate immune response of neonicotinoid-resistant pests against high doses of insecticides.

Novel Eel Skin Fibroblast Cell Line: Bridging Adherent and Suspension Growth for Aquatic Applications Including Virus Susceptibility.

Suspension growth can greatly increase the cell density and yield of cell metabolites. To meet the requirements of aquatic industries, a culture model derived from skin was developed using the explant outgrowth and enzyme-digesting passaging methods. These cells were kept in vitro continuously for over 12 months and subcultured 68 times.

Genome-Wide Structural Variation Analysis and Breed Comparison of Local Domestic Ducks in Shandong Province, China.

Structural variations in the duck genome significantly impact the environmental adaptability and phenotypic diversity of duck populations. Characterizing these SVs in local domestic duck breeds from Shandong province offers valuable insights for breed selection and the development of new breeds. This study aimed to profile the genomic SVs in three local duck breeds (Matahu duck, Weishan partridge duck, and Wendeng black duck) and explore their differential distributions.

A review on molecularly imprinted magnetic solid phase extraction emphasizing the analysis of antibiotics in complex matrices:Design, preparation, and application.

Magnetic solid phase extraction (MSPE) has been widely employed in the isolation and enrichment of antibiotics in complex matrices because it presents various unique advantages over traditional SPE including simple operation, fast extraction procedure, low cost and eco-friendliness. In recently years, magnetic molecularly imprinted nanoparticles (MMINs) containing highly specific recognition performance have been widely used to specific extraction of antibiotics under the format of MSPE. In this connection, recent advances of MMINs in the analysis of antibiotic residues are reviewed.

CtWD40-6 enhances the UV-B tolerance of safflower by regulating flavonoid accumulation.

Moderate UV-B promotes plant growth, but excessive UV-B inhibits plant development. The induction mechanism of how CtWD40-6 responds to UV-B is still unclear in safflower. Our results showed that CtWD40-6 is expressed at the top of safflower leaves and is strongly induced by UV-B.

STMGraph: spatial-context-aware of transcriptomes via a dual-remasked dynamic graph attention model.

Spatial transcriptomics (ST) technologies enable dissecting the tissue architecture in spatial context. To perceive the global contextual information of gene expression patterns in tissue, the spatial dependence of cells must be fully considered by integrating both local and non-local features by means of spatial-context-aware. However, the current ST integration algorithm ignores for ST dropouts, which impedes the spatial-aware of ST features, resulting in challenges in the accuracy and robustness of microenvironmental heterogeneity detecting, spatial domain clustering, and batch-effects correction.

Sustainable aviation fuel (SAF) from lignin: Pathways, catalysts, and challenges.

The aviation industry plays a crucial role in global trade and cultural exchange, but it faces significant challenges due to high production cost and environmental impacts. To achieve carbon neutrality, promoting the development of sustainable aviation fuel (SAF) is essential, with projections indicating that 65% of emissions reductions in the aviation industry by 2050 will come from the use of SAF. Lignin, as an abundant renewable resource, has great potential for conversion into aviation fuel components.

The NAC transcription factor LpNAC48 promotes trichome formation in Lilium pumilum.

Trichomes play a crucial role in plant resistance to abiotic and biotic stresses, and their development and characteristics vary across different species. This study demonstrates that trichomes of Lilium pumilum exhibit synchronized growth during flower bud differentiation and enhance the plant's adaptability to UV-B radiation and aphid infection. We identified LpNAC48, a NAC family transcription factor (TF), that interacted with the B-box (BBX) family TF LpBBX28, during trichome formation in L.

Haplotype-resolved genome of a papeda provides insights into the geographical origin and evolution of Citrus.

The publication of several high-quality genomes has contributed greatly to clarifying the evolution of citrus. However, due to their complex genetic backgrounds, the origins and evolution of many citrus species remain unclear. We assembled de novo the 294-Mbp chromosome-level genome of a more than 200-year-old primitive papeda (DYC002).

Solvent induced method for preparation of multi-advantage carrageenan films.

Carrageenan has good film-forming characteristics, but it is difficult to simultaneously improve its multiple performances, such as water-resistance, light transmittance and thermal stability. In this study, multi-advantage composite films were prepared by iota-carrageenan and quaternary ammonium surfactants according to solvent induced method. The weight change, FTIR and thermogravimetric analyses of the films before and after solvent inducement indicated that the inorganic counterions of iota-carrageenan were replaced by quaternary ammonium ions.

Amplified impacts of human activities: Non-linear responses of riverine microbial communities to distribution of land use.

Rapid global urbanization poses considerable ecological risks to freshwater systems, notably leading to substantial reductions in microbial communities. To assess the impacts of human activities on these communities, we applied the high-throughput amplicon DNA sequencing to examine spatial variations in riverine microbial communities within an urbanized watershed. Coupled with the Geographical Detector Model, the effects of the land use were identified across the watershed.

Effects of storage durations on flavour and bacterial communities in Liupao tea.

Long-term storage of Liupao tea is conducive to improving its flavour and commercial value. Although bacterial communities influence Liupao tea flavour, their impact during storage remains unclear. The aroma compounds and bacterial communities were determined by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and Illumina Nova6000 analysis.

Integrating ecological and recreational functions to optimize ecological security pattern in Fuzhou City.

The scientific establishment of the Ecological Security Pattern (ESP) is crucial for fostering the synergistic development of ecological and recreational functions, thereby enhancing urban ecological protection, recreational development, and sustainable growth. This study aimed to propose a novel method of constructing ESP considering both ecological and recreational functions, and to reconstruct ESP by weighing the relationship between ecological protection and recreational development. Utilizing Fuzhou City as a case study, a comprehensive application of methodologies including Morphological Spatial Pattern Analysis (MSPA), landscape connectivity analysis, ArcGIS spatial analysis, social network analysis (SNA), and circuit theory is employed to develop both the ESP and the Recreational Spatial Pattern (RSP).

Pointer meters recognition method in the wild based on innovative deep learning techniques.

This study presents a novel approach to identifying meters and their pointers in modern industrial scenarios using deep learning. We developed a neural network model that can detect gauges and one or more of their pointers on low-quality images. We use an encoder network, jump connections, and a modified Convolutional Block Attention Module (CBAM) to detect gauge panels and pointer keypoints in images.

Rapid and accurate multi-phenotype imputation for millions of individuals.

Deep phenotyping can enhance the power of genetic analysis, including genome-wide association studies (GWAS), but the occurrence of missing phenotypes compromises the potential of such resources. Although many phenotypic imputation methods have been developed, the accurate imputation of millions of individuals remains challenging. In the present study, we have developed a multi-phenotype imputation method based on mixed fast random forest (PIXANT) by leveraging efficient machine learning (ML)-based algorithms.

FADD cooperates with Caspase-8 to positively regulate the innate immune response and promote apoptosis following bacterial infection in Japanese eel.

Fas-associated protein with Death Domain (FADD) is a crucial signaling component of apoptosis and a vital immunomodulator on inflammatory signaling pathways. However, information on FADD-mediated apoptosis and immune regulation is limited in teleost. We herein cloned a FADD homolog, AjFADD, from Japanese eel (Anguilla japonica).