[Evaluation of the preclinical teaching effects of digital virtual simulation system in veneer tooth preparation].

Purpose: To evaluate the preclinical teaching effects of digital virtual simulation system applied in window-type veneer tooth preparation of undergraduate dental students.

Methods: In the early clinical exposure of five-year undergraduate students from School of Stomatology, Fudan University, Simodont virtual simulation system and conventional phantom-simulator system were alternatively used in the preclinical teaching of veneer tooth preparation. First, after theoretical training and tests of veneer tooth preparation, twenty-three students were randomly divided into two groups.

[Application of PDCA theory in prosthodontic for standardized training of dental residents].

Purpose: To evaluate the application effect of PDCA cycle teaching mode in the standardized training and teaching of dental residents in prosthodontics speciality.

Methods: A total of 46 dental residents in Shanghai Stomatological Hospital from 2020 to 2022 were selected as the research subjects, and divided into experimental group (n=23) and control group(n=23), the control group adopted conventional teaching method, while the experimental group adopted PDCA cycle teaching method on the basis of the control group. The theoretical assessment, clinical operation assessment and satisfaction of the teaching effect of the two groups of residents were compared with SPSS 20.

[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].

Purpose: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.

Methods: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks.

[Evaluation of the effect of secondary functional impression complete denture on improving oral health of edentulous patients].

Purpose: To evaluate the clinical effect of complete denture restoration in edentulous patients using secondary functional impression.

Methods: Complete denture restoration was performed in 433 edentulous patients using secondary functional impression. The Geriatric Oral Health Assessment Index (GOHAI) was used to evaluate patients' oral health-related quality of life before and after restoration.

[Monkeypox transmission and oral prevention].

Monkeypox is becoming a viral infectious disease of global concern. WHO has reported monkeypox outbreaks in more than 50 countries. Since the first imported case has been confirmed and reported by Taiwan, China, in June 2022, the monkeypox has draw high attention from the national public health and epidemic prevention department.

[Application of nitrous oxide/oxygen inhalation comfort technique during tooth extraction of elderly hypertensive patients under electrocardiographic monitoring].

Purpose: To investigate the application value of nitrous oxide/oxygen inhalation comfort technique during tooth extraction in elderly patients with hypertension under electrocardiographic(ECG) monitoring.

Methods: According to the inclusion and exclusion criteria, sixty elderly patients (over 65 years old) with hypertension for tooth extraction were randomly divided into 2 groups: the experimental group(nitrous oxide/oxygen inhalation combined with ECG monitoring group, n=30) and the control group (routine ECG monitoring group, n=30). Mean arterial pressure (MAP) and heart rate (HR) at T0 (baseline values before surgery), T1 (on local anesthesia), T2 (during operation) and T3(5 minutes after operation) were recorded.

[Prevalence of torque teno mini virus (TTMV) and epstein-barr virus (EBV) in gingival tissues in 80 patients with periodontitis].

Purpose: To study the prevalence of torque teno mini virus (TTMV) and epstein-barr virus(EBV) in patients with periodontitis.

Methods: Gingival tissue samples were collected from 80 patients with periodontitis and 40 periodontal healthy volunteers. The presence of EBV and TTMV-222 were detected by nested PCR, and the virus loads were detected by real-time PCR.

[Risk factors for the presence of dental black stain in 82 children].

Purpose: To investigate the prevalence of dental black stain in children and analyze the risk factors of dental black stain.

Methods: We conducted this investigation through a case-control study. A total of 164 children (82 children with dental black stain and 82 children without dental black stain) were recruited from Shanghai Stomatological Hospital.

[Application of two different comfort techniques in extraction of impacted teeth among 60 patients with hypertension].

Purpose: To compare the effect of two comfort techniques of nitrous oxide/oxygen inhalation and dexmedetomidine combined with flurbiprofen axetil sedation and analgesia in impacted teeth extraction among patients with hypertension.

Methods: According to the inclusion and exclusion criteria, sixty patients undergoing impacted teeth extraction with hypertension were randomly divided into 2 groups: X group( nitrous oxide/oxygen inhalation, n=30) and Y group(dexmedetomidine combined with flurbiprofen axetil assisted sedation and analgesia, n=30). The following parameters at T0(preoperative), T1(local anesthesia), T2 (surgical operation), and T3 (5 minutes after surgery) were recorded: mean arterial pressure(MAP), heart rate (HR), Ramsay sedation score, VAS pain score.

[Human resources of community oral public health service and job satisfaction of the staff in Shanghai].

Purpose: To investigate the human resources of oral public health system and staff's job satisfaction in Shanghai, and to provide basis for improving the services of oral public health.

Methods: A census was performed among 246 health service centers in Shanghai from February to March 2019, and a questionnaire on oral public health staff's basic information of and job satisfaction was conducted. SPSS 20.

[Effect of strontium ranelate on chondrogenic differentiation of rat bone mesenchymal stem cells].

Purpose: The aim of present study was to explore the effect of strontium ranelate (SrR) on the proliferation and chondrogenic differentiation of rat bone mesenchymal stem cells (BMSCs).

Methods: Rat BMSCs were isolated and cultured in chondrogenic differentiation medium containing 0.25-2.

[Orofacial myofunctional therapy improves facial morphology of children with obstructive sleep apnea after adenotonsillectomy].

Purpose: This study investigated the effectiveness of orofacial myofunctional therapy(OMT) in improving facial morphology of children with obstructive sleep apnea (OSA) after adenotonsillectomy (AT).

Methods: Ten children aged from 4-7 years with persistent oral breathing for more than 1 month after adenotonsillectomy were chosen to receive orofacial myofunctional therapy. The patients were required to take photos before and after orofacial myofunctional therapy.

[Survey on oral health knowledge, attitudes, behaviors among parents of disabled children in Shanghai area].

Purpose: To analyze the oral health behaviors of disabled children and their parents' oral health knowledge, attitudes in Shanghai city, and to provide information support for designing oral health care programs and making relevant policies.

Methods: By using stratified cluster sampling method, a questionnaire was given to 1381 parents of disabled children. The data were analyzed using SPSS 21.

[Evaluation of the application of dexmedetomidine combined with flurbiprofen axetil in extraction of multiple complex teeth under local anesthesia].

Purpose: To evaluate the safety and efficacy of sedation and analgesia using dexmedetomidine combined with flurbiprofen axetil in multiple complex teeth extraction under local anesthesia.

Methods: According to the inclusion and exclusion criteria of the study, 40 patients scheduled for multiple complex teeth (4-6) extraction were randomly divided into 2 groups: experimental group (sedation and analgesia using dexmedetomidine combined with flurbiprofen axetil in addition to local anesthesia, n=20) and control group (local anesthesia, n=20). The mean arterial pressure(MAP), heart rate(HR), Ramsay sedation score, VAS pain score of each patient at T0(basis value), T1 (during local anesthesia), T2(during extraction), T3(10 minutes after extraction) and the follow-up results were recorded.

[Study of the root position of palatally malposed maxillary lateral incisor based on cone-beam CT images].

Purpose: The goal of this investigation was to measure and analyze the root position of palatally malposed maxillary lateral incisor based on cone-beam CT(CBCT)images, in order to provide references for orthodontists to move this kind of teeth scientifically.

Methods: CBCT data from 200 patients meeting the selection criteria with palatally malposed maxillary lateral incisor were investigated in this study. The root was divided into eight equal parts by length, then T1 to T8 were orderly pointed from root apex to alveolar ridge crest.

[Human dental pulp stem cells conditioned medium protects genioglossus myoblast from cobalt chloride-induced hypoxia injury through AMPK/PGC-1α pathway].

Purpose: To study the effect of hypoxia induced by cobalt chloride (CoCl2) on viability and oxidative stress of genioglossus myoblast, and to explore the mechanism of the protective effect of conditioned medium (CM) on human dental pulp stem cells (hDPSCs).

Methods: The hDPSCs were isolated and cultured, and the conditioned medium was prepared by ultrafiltration concentration. Mouse genioglossus myoblasts were isolated and divided into control group, CM group, CoCl2 group and CoCl2+CM group.

[Influence of vitexin on the expression of inflammatory cytokines in dental pulp stem cells induced by lipopolysaccharide].

Purpose: To investigate the effect of vitexin (VTX) on the expression of inflammatory cytokines in human dental pulp stem cells(hDPSCs) induced by lipopolysaccharide(LPS), and to explore the underlying mechanism.

Methods: hDPSCs were isolated and cultured, and CCK-8 method was used to detect the effect of VTX on proliferation of hDPSCs. hDPSCs were randomly divided into 4 groups: blank group (without LPS and VTX)，LPS group (2 μg/mL LPS)，2 μg/mL LPS + 25 μmol/L VTX，2 μg/mL LPS + 50 μmol/L VTX.

[Biological characteristics of periodontal ligament stem cells modified by platelet derived growth factor BB].

Purpose: To investigate the biological characteristics of human periodontal stem cells (hPDLSCs) modified with platelet derived growth factor BB(PDGFBB) gene, and to explore its influence on proliferation, migration and osteogenic induction of hPDLSCs.

Methods: hPDLSCs were isolated and amplified, and immunofluorescence staining was performed to identify cell surface markers and osteogenic differentiation ability. hPDLSCs were transfected with PDGFBB gene by lentivirus vector, and the effects on cell proliferation and migration were detected by CCK-8 and scratch test after transfection.

[Effect of oral health education with children popular oral science short drama on oral health care KAP among 10-year-old children].

Purpose: To evaluate the impact of health education with children popular oral science short drama on 10-year-old children's oral health knowledge, attitude, practice (KAP), and provide evidence for oral health education methods for children.

Methods: A oral health education short drama for children was filmed. 10-year-old children from a primary school in Minhang district, Shanghai were selected as the study subjects.

[Early childhood caries and its related risk factors in 1 296 children aged 3 to 5 years old in Shanghai].

Purpose: To investigate the prevelence of early childhood caries (ECC) and its related risk factors in children aged 3 to 5 years old in Shanghai.

Methods: According to the requirements of the Fourth National Epidemiological Survey of Oral Health, multistage, stratified, equal capacity and simple random sampling method was performed. A total of 1 296 children aged 3, 4 and 5 years old in 12 kindergartens in Shanghai were selected to calculate the prevalence and the mean dmft(decayed-missing-filled tooth).

[Epidemiological investigation and analysis of risk factor of tooth loss in older people aging 65-74 years in Shanghai].

Purpose: To investigate tooth loss status and associated risk factors in older adults aging 65-74 years in Shanghai.

Methods: Seven hundred and seventy-six participants aging between 65-74 years were randomly selected in a cross-sectional study according to the requirements of the fourth national oral health epidemiological investigation of China. Data was obtained via questionnaires and clinical examination.