2 results match your criteria: "Fudan University Affiliated Eye Ear Nose and Throat Hospital[Affiliation]"
[Isolation and in vitro characterization of CD133(+) side population cells from laryngeal cancer cell line].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
September 2011
Department of Otorhinolaryngology Head and Neck Surgery, Fudan University Affiliated Eye Ear Nose and Throat Hospital, Shanghai 200031, China.
Article Synopsis
- The study aims to find a better method for enriching cancer stem cells (CSCs) from a laryngeal cancer cell line using specific cell sorting techniques.
- Researchers used fluorescence activated cell sorting to isolate CD133(+)SP and CD133(-)SP subpopulations from the Hep-2 cell line, examining their growth and resistance to treatment.
- Results showed that CD133(+)SP cells, despite being a small fraction, demonstrated significantly faster proliferation, greater resistance to chemotherapy, and superior CSC-like properties compared to CD133(-)SP cells, indicating their potential for further research in laryngeal cancer.
[Investigation of CD133 as putative marker of tumor-initiating cell in laryngeal carcinoma].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
September 2007
Department of Otorhinolaryngology Head and Neck Surgery, Fudan University Affiliated Eye Ear Nose and Throat Hospital, Shanghai 200031, China.
Objective: To detect the expression of CD133 in human established larynx tumors cell line, Hep-2 cell line and observe tumorigenicity of CD133(+) tumorigenic cells, CD133(-) tumorigenic cells and unsorted Hep-2 cells in vivo. The marker of cancer stem cells in Hep-2 cell line was explored.
Methods: Flow cytometry was used to detect the expression of putative tumor-initiating cell marker CD133 in Hep-2 cell line.