Shortcoming of ypStage staging system: Lack of differentiation for preoperative treatment.

The eighth edition of the AJCC staging manual initially proposed the ypTNM staging system, which was specifically designed to assess the staging and predict the prognosis of cancer patients undergoing preoperative treatment. Nevertheless, it remains unclear whether this staging system is an accurate predictor of outcomes for cancer patients undergoing different preoperative treatments. The clinical and pathological data of gastric cancer patients who received preoperative treatment and subsequent curved tented gastrostomy were obtained from the Surveillance, Epidemiology, and End Results database.

Effect of Molecular Weight of Fluorescent Dyes on DNA Separation by Capillary Electrophoresis.

Capillary electrophoresis (CE) plays an important role in the quality control of dsDNA. So far, there has been various fluorescent dyes employed for the separation of dsDNA by CE. However, the molecular weight of the dyes may affect the mass to charge ratio of dsDNA-dye complex, consequently the separation performance of dsDNA will be changed.

Pharmacokinetic, Pharmacokinetic/Pharmacodynamic, and Safety Investigations of Cefiderocol in Chinese Healthy Subjects.

Introduction: We aim to evaluate the safety and pharmacokinetic (PK) properties of cefiderocol in Chinese participants, following single and subsequent multiple administrations of 2 g q8h with 3-h intravenous infusion, and to predict its efficacy for the treatment of Gram-negative bacilli (GNB) infection based on PK/pharmacodynamic (PD) analysis.

Methods: This was an open-label, single-center, single- and multiple-dose phase I study, conducted from September 2022 to October 2022, with 12 eligible healthy Chinese adults (6 men and 6 women). The PK profiles were described by noncompartmental analysis and a two-compartment model using WinNonlin (v.

Study on Effect of Different Pulses of rTMS on Visual Working Memory in Elderly With SCD.

Previous research has shown that rTMS improves visual working memory (VWM) performance in older people with subjective cognitive decline (SCD). However, the influence of stimulation parameters on the effect is unclear. We focus on the total number of stimulus pulses and aim to investigate whether 10 Hz rTMS with different total pulses could have different effects on VWM in SCD subjects.

Enantioselective Total Synthesis of Fortimicin B.

Fortimicins, featuring with a pseudodisaccharide scaffold, are an unusual class of aminoglycosides with potent efficacy against several aminoglycoside-resistant bacterial strains. Notably, these molecules also exhibit lower inherent ototoxicity and nephrotoxicity than common aminoglycosides. Consequently, fortimicins are a promising type of protypical molecules for the development of the next-generation of aminoglycoside antibiotics.

Tas2r105 ameliorates gut inflammation, possibly through influencing the gut microbiota and metabolites.

Inflammatory bowel disease (IBD) is an immune-mediated gastrointestinal disorder that significantly impacts the life quality of people worldwide. Genetic factors play crucial roles in the development of IBD. Tas2rs, members of the G protein-coupled receptor (GPCR) superfamily, are known for their roles in bitter taste perception.

A Rigorous Nuclear DNA-Excluding Mass Spectrometry Assay for Accurate Identification of 5-Methylcytosine in Mitochondrial DNA.

5-Methylcytosine (5mC) functions as a well-characterized epigenetic DNA mark in nuclear DNA, but its presence in mitochondrial DNA (mtDNA) remains elusive. Here, we report a new and rigorous nuclear DNA (nDNA)-excluding mass spectrometry assay enabling the reliable and accurate identification of 5mC in mtDNA for the first time. First, circular mtDNA is enriched over 809-946-fold by combining alkaline lysis and linear DNA-specific RecBCD cutting; nDNA accounts for ∼12-19% of the DNA remaining after this step.

March2 Alleviates Aortic Aneurysm/Dissection by Regulating PKM2 Polymerization.

Background: Aortic aneurysm/dissection (AAD) is a life-threatening disease lacking effective pharmacological treatment. Protein ubiquitination plays a pivotal role in cardiovascular diseases. However, the possible contribution of the E3 ubiquitin ligase March2 (membrane-associated RING finger protein 2) to the cause of AAD remains elusive.

Cascade-Responsive Nanoprodrug Disrupts Immune-Fibroblast Communications for Potentiated Cancer Mechanoimmunotherapy.

The abnormal tumor mechanical microenvironment due to specific cancer-associated fibroblasts (CAFs) subset and low tumor immunogenicity caused by inefficient conversion of active chemotherapeutic agents are two key obstacles that impede patients with desmoplastic tumors from achieving stable and complete immune responses. Herein, it is demonstrated that FAP-αCAFs-induced stromal stiffness accelerated tumor progression by precluding cytotoxic T lymphocytes. Subsequently, a cascade-responsive nanoprodrug capable of re-educating FAP-αCAFs and amplifying tumor immunogenicity for potentiated cancer mechanoimmunotherapy is ingeniously designed.

Intermittent fasting inhibits platelet activation and thrombosis through the intestinal metabolite indole-3-propionate.

Platelet hyperreactivity contributes significantly to thrombosis in acute myocardial infarction and stroke. While antiplatelet drugs are used, residual ischemic risk remains. Intermittent fasting (IF), a dietary pattern characterized by alternating periods of eating and fasting, has shown cardiovascular benefits, but its effect on platelet activation is unclear.

Up IGF-I via high-toughness adaptive hydrogels for remodeling growth plate of children.

The growth plate is crucial for skeletal growth in children, but research on repairing growth plate damage and restoring growth is limited. Here, a high-toughness adaptive dual-crosslinked hydrogel is designed to mimic the growth plate's structure, supporting regeneration and bone growth. Composed of aldehyde-modified bacterial cellulose (DBNC), methacrylated gelatin (GelMA) and sodium alginate (Alg), the hydrogel is engineered through ionic bonding and Schiff base reactions, creating a macroporous structure.

Healthcare workers' knowledge, attitudes, and practices on catheter-associated UTI prevention: influencing factors in an OB/GYN hospital.

Background: Catheter-associated urinary tract infection (CAUTI) is a leading cause of hospital-acquired infections globally, with a high prevalence in China, especially in high-risk settings like intensive care and post-operative environments. These infections are influenced by factors such as patient volume, healthcare worker training, and adherence to infection control protocols. Shanghai, as a major healthcare hub, faces unique challenges in CAUTI prevention.

Full genomic sequence characterization of the chikungunya virus from an imported case with serum viral concentration below culturable level.

Chikungunya virus (CHIKV) infection, responsible for chikungunya fever and occasionally severe symptoms, has emerged as an increasing global health concern following several large-scale outbreaks from Africa, Asia, Europe, and America. Over the past two decades, South and Southeast Asia regions have gradually become hot spots for outbreaks involving multiple CHIKV lineages. In China, most CHIKV infections are imported, making it crucial to trace the origins and transmission routes for effective prevention and control.

Evaluation of antibody responses in healthy individuals receiving SARS-CoV-2 inactivated vaccines.

Inactivated coronavirus disease 2019 (COVID-19) vaccines such as CoronaVac and BBIBP-CorV have been widely used in China. However, more investigation is still needed to understand antibodies' duration and effectiveness against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in the real world. In this study, 575 participants who had been vaccinated with two or three doses of the inactivated vaccine were recruited.

Caspase-8 activation regulates enterovirus D68 infection-induced inflammatory response and cell death.

Enterovirus D68 (EV-D68) infection causes severe acute respiratory infection and severe neurological complications, such as acute flaccid myelitis (AFM), in children. However, although EV-D68 has pandemic potential, no effective drugs or vaccines are currently clinically available. Furthermore, EV-D68 infection-induced inflammatory response and cell death are not fully understood.

The characteristics of brain function alterations in patients with chronic prostatitis/chronic pelvic pain syndrome across varying symptom severities evaluated by NIH-CPSI.

Background: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a prevalent condition in urology characterized by chronic pain. The pathogenesis of CP/CPPS remains unclear.

Methods: We enrolled 45 eligible CP/CPPS patients and 45 healthy volunteers.

Protective and risk factors in daily life associated with cognitive decline of older adults.

Background: Cognitive decline is a chronic condition which is characterized by a loss of the ability to remember, learn, and pay attention to complex tasks. Many older people are now suffering from cognitive decline, which decreases life quality and leads to disability. This study aimed to identify the risk and protective factors for cognitive decline of the older people from daily life and establish a predictive model using logistic regression.

Clinicopathological and molecular features of HR /HER2 breast cancer patients with distinct endocrine resistance patterns.

Objective: Recurrence continues to be a pivotal challenge among hormone receptor-positive (HR)/human epidermal growth factor receptor 2-negative (HER2) breast cancers. In the international consensus guidelines, HR/HER2 breast cancer relapse patterns are divided into three distinct types: primary resistant, secondary resistant, and endocrine sensitive. However, owing to the lack of cohorts with treatment and follow-up data, the heterogeneity among different recurrence patterns remains uncharted.

Integrated analysis of single-cell and bulk transcriptomes uncovers clinically relevant molecular subtypes in human prostate cancer.

Objective: Prostate cancer (PCa) is a complex disease characterized by diverse cellular ecosystems within the tumor microenvironment (TME) and high tumor heterogeneity, which challenges clinically stratified management and reinforces the need for novel strategies to fight against castration-resistant PCa (CRPC).

Methods: We performed single-cell RNA sequencing (scRNA-seq) on 10 untreated primary PCa tissues and integrated public scRNA-seq resources from three normal prostate tissues, two untreated primary PCa tissues, and six CRPC tumors to portray a comprehensive cellular and molecular interaction atlas of PCa. We further integrated the single-cell and bulk transcriptomes of PCa to establish a molecular classification system.

mutations in with negative MPT64 antigen assay results from a tertiary hospital in Southeastern China.

Background: MPT64 protein is an effective marker for detecting (MTB) in liquid culture and clinical tissue samples. However, some MTB clinical isolates test negative for this antigen because of varied mutation types across different regions.

Methods: DNA samples of MPT64 antigen assay-negative MTB strains were collected from a tertiary hospital from January 2016 to January 2024, and gene mutations were detected by sequencing.

Gentiopicroside targeting AKT1 activates HIF-1α/VEGF axis promoting diabetic ulcer wound healing.

Backgound: Gentiopicroside (GSP) have been proven to accelerate the healing of diabetic ulcers (DU), but the underlying molecular mechanisms remain unclear. This study aims to explore the mechanism by which GSP accelerates the healing of DU.

Method: The targets of GSP were firstly predicted using the SuperPred, SwissTargetPrediction, and Pharmmapper databases; DU-related transcriptome data were obtained from the GEO database, including GSE147890, GSE68183, and GSE199939; differential expression analysis was conducted using the Limma package, and DU-related targets were identified after summarization and de-duplication.

Binocular vHIT in Healthy Participants: An Optimized Strategy.

Objective: The objectives of this study were to observe the differences between the left and right eyes in healthy individuals during binocular video head impulse testing (vHIT) and to identify the optimal eye for a more precise evaluation of the vestibulo-ocular reflex (VOR) gain.

Materials And Methods: This is a prospective two-center study involving 41 healthy individuals. All participants included in the study underwent binocular vHIT, which was performed by experienced technicians.

Molecules Targeting EriC1 Increase Fluoride Sensitivity.

Dental caries, as one of the prevalent oral infectious diseases worldwide, constitutes a considerable disease burden. Fluoride has been widely used to prevent dental caries for decades. However, fluoride alone may not always be sufficient.

Eye-sparing treatment for sinonasal tumors invading the orbit in the era of personalized therapy.

Few studies investigated the eye-sparing treatment in patients with sinonasal carcinomas (SNCs) from the perspective of modern precision radiation, and the eye-sparing treatment in patients with grade III orbital invasion was rarely discussed. This retrospective study included patients with primary SNCs who accepted eye-sparing treatment from October 2000 to June 2022 at a single institution. Patients' clinical information and follow-up data (including visual data) were obtained.

De Novo Design of Large Polypeptides Using a Lightweight Diffusion Model Integrating LSTM and Attention Mechanism Under Per-Residue Secondary Structure Constraints.

This study presents PolypeptideDesigner (PPD), a novel conditional diffusion-based model for de novo polypeptide sequence design and generation based on per-residue secondary structure conditions. By integrating a lightweight LSTM-attention neural network as the denoiser within a diffusion framework, PPD offers an innovative and efficient approach to polypeptide generation. Evaluations demonstrate that the PPD model can generate diverse and novel polypeptide sequences across various testing conditions, achieving high pLDDT scores when folded by ESMFold.