Histoplasmosis in a Solid Organ Transplant Patient in a Non-Endemic Region in New York City.

Objective: To present the case of a solid organ transplant recipient with Histoplasmosis in New York City.

Case Report: We present a 39-year-old female liver transplant recipient, who experienced a two-week history of progressive shortness of breath and dyspnea on exertion that interfered with all activities of daily living. Physical examination by the team revealed the patient had a WBC of 11.

Effect of CXCR2 Deficiency in HeLa Cell on the Regulatory Network of Coding Genes and Non-Coding RNAs.

Objective: C-X-C motif chemokine receptor 2 (CXCR2) plays a crucial role in inflammation and immunity, and the involvement of chemokine receptors in the tumor microenvironment is extensively documented. However, the impact of CXCR2 deficiency on the complete transcriptome, including mRNA and ncRNAs, in tumor cells remains unclear.

Methods: In this study, we aimed to identify differentially expressed (DE) messenger RNA (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in CXCR2 knockout HeLa cells through transcriptome sequencing and to construct regulatory networks.

Effects of MIR-451 on LKB1-AMPK Signal Pathway and Oxidative Stress Index in Lung Cancer.

Objective: To understand the influence of MIR-451 on LKB1-AMPK signal pathway and oxidative stress index in lung cancer mice.

Methods: 40 rats were divided into four groups: ZC group (no lung cancer model), model group (lung cancer model), intervention group (rats with MiR-451 agomir injected), and NC group (rats with MiR-451 agomir control injected), 10 mice/group. The levels of MDA (malondialdehyde) and SOD (superoxide dismutase) in the four groups were detected, as well as the overall weights of the lungs and spleens.

Cornus Officinalis Iridoid Glycosides Protect against Chronic Renal Failure in Rats by Activating the Nrf2-antioxidant Pathway.

Objective: To investigate the therapeutic effects of Cornus officinalis iridoid glycosides (CIG) on rats with chronic renal failure (CRF).

Methods: CRF was induced in adult male Sprague Dawley rats by nephrectomy. The rats were randomly divided into six groups: sham, sham+high-dose CIG (120 mg/kg/d for 14 days), CRF, CRF+low-dose CIG (60 mg/kg/d for 14 days), CRF+high-dose CIG, and CRF+high-dose CIG+ML385 (an inhibitor of nuclear factor erythroid 2-related factor 2 (Nrf2), single administration at 30 mg/kg).

A Challenging Case of Myeloid Sarcoma Misdiagnosed as High-Grade B-Cell Lymphoma.

A 46-year-old female presented with back pain associated with progressive bilateral lower extremity weakness and paresthesia. Imaging studies revealed a retroperitoneal mass with severe spinal compression. Histological sections showed blastoid cells with large nuclei, irregular membranes, fine chromatin, and prominent nucleoli.

Barriers to Blood Donation: Donor Deferrals in a Saudi Hospital Setting.

Objective: Blood donation plays a critical role in healthcare systems by supplying blood and blood products for surgeries, emergency care, and chronic disease management. In Saudi Arabia, the prevalence of genetic disorders, like sickle cell disease and thalassemia, further increase the need for a stable blood supply. However, high rates of donor deferrals remain a significant barrier to maintaining a reliable blood supply.

Abietic Acid Alleviates the Hypoxic Injury of Cardiomyocytes by Adjusting Autophagy and Apoptosis Mediated by miR-30a-5p/GRP78 Axis.

Objective: To explore the influence of abietic acid on the autophagy and apoptosis of cardiomyocytes in rats with acute myocardial infarction (AMI).

Methods: A rat model of AMI was built by ligation of the anterior descending branch of left coronary artery, and a model of hypoxic cardiomyocyte injury was constructed by treating cardiomyocytes with hypoxia. Western blot assay was used to detect the abundance of proteins related to autophagy and apoptosis, MTT assay was used to measure the viability of cardiomyocytes, and the expression level of miR-30a-5p was detected by qRT-PCR.

Optimizing UroVysion Fluorescence Hybridization Reflex Testing in Atypical Urothelial Cell Diagnosis: A Comprehensive Cytomorphological Analysis.

Objective: Urinary cytology is a key diagnostic tool for evaluating suspected urinary tract carcinoma, primarily high-grade urothelial carcinoma. The Paris System for Reporting Urinary Cytology (TPS), introduced in 2016, aimed to standardize reporting, though challenges with subjectivity and variability in diagnosing Atypical Urothelial Cells (AUCs) persist.

Methods: This retrospective study explored the correlation between cytomorphological features in "atypical" diagnosis and UroVysion fluorescence hybridization (U-FISH) results.

Se-methylselenocysteine Inhibits Migration and Glycolysis in Anaplastic Thyroid Carcinoma Cells via the ERK1/2 Signaling Pathway .

Objective: The aim of the present study was to determine the effects of selenium-methylselenocysteine (MSC) on the viability, migration, and glycolysis of human ATC cell lines 8305 and BHT101 .

Methods: Cells were treated with MSC and viability was determined using the Cell Counting Kit 8 assay. The migratory ability of cells was detected using a Transwell migration assay, and the expression levels of proteins involved in the ERK1/2, JNK, and p38 signaling pathways were measured by western blotting.

Tiam1 Mediated Enhancement of AKT/mTOR and ERK/STAT3 Signaling Promotes Proliferation, Invasion and Migration of Pancreatic Cancer.

Objective: To explore the role of Tiam1 in proliferation, invasion, and migration of pancreatic cancer.

Significance: Previous studies have shown that T-cell lymphoma invasion and metastasis-inducing factor 1 (Tiam1) is involved in multiple tumor progression. However, the role and molecular mechanism of this molecule in pancreatic cancer remain unclear.

A Patient with a Delayed Diagnosis of Zinc Powder Inhalation Poisoning: A Case Report.

Objective: Overexposure to zinc can lead to zinc poisoning. Zinc poisoning from zinc powder inhalation has rarely been reported.

Case Report: A 66-year-old male presented with nausea, chest tightness, and shortness of breath.

Identification of lipid metabolism-related gene markers and construction of a diagnostic model for multiple sclerosis: An integrated analysis by bioinformatics and machine learning.

Background: Multiple sclerosis (MS) is an autoimmune inflammatory disorder that causes neurological disability. Dysregulated lipid metabolism contributes to the pathogenesis of MS. This study aimed to identify lipid metabolism-related gene markers and construct a diagnostic model for MS.

Calculation of lifetime relative risks from summary cohort data and application to calculation of attributable fractions.

In cohort studies, relative risks are estimated by comparing eventual disease risk in individuals exposed to the risk factor at baseline with similar unexposed individuals. However, such relative risk estimates intrinsically depend on how many of the unexposed individuals develop exposure after baseline and on the ages at which the exposed individuals developed exposure prior to baseline. These factors pertain to the distribution of risk factor incidence in the population, rather than to the causal effect the risk factor has on disease.

Development and temporal-validation of prognostic models for 5-year risk of pneumonia, respiratory failure/collapse, and fracture among adults with cerebral palsy.

Purpose: Pneumonia, respiratory failure, and fracture are common and highly burdensome for adults with cerebral palsy (CP). To date, there are no clinically friendly tools to assess individualized risk of these outcomes for adults with CP. The objective was to develop and validate prognostic models for 5-year risk of pneumonia, respiratory failure/collapse, and fracture for adults with CP.

Investigating the Binding Dynamics and Stability of Bovine Serum Albumin with Antibiotics with Acridinedione Dye.

Exploring the energetics and bimolecular interaction of Bovine Serum Albumin (BSA) with various classes and generations of antibiotics in the absence and presence of a resorcinol based acridinedione dye (ADR1) were carried out. The binding stability of BSA-antibiotic complexes decreases on the introduction of ADR1 dye resulting in a positive value of free energy change, accompanied with several unfavourable interactions. Several polar amino acids contributed to the stability of the host-guest complex compared to that of non-polar amino acids, wherein BSA acts as the host, and antibiotics as the guest and ADR1 dye as the competing guest molecule.

[Review of practices concerning the delivery of an international implant card following the implantation of a medical device.].

Introduction: The European regulation 2017/745 requires the delivery of an international implant card to the patient following the implantation of implantable medical devices (IMD). Currently, there are no procedure to describe the process for providing traceability documents to patients in our institution. The objective of this work was to evaluate the practices of the various departments concerned in our institution and to think about actions to meet the current regulation.

Simulation-based training improves developmental hip dysplasia examination and diagnosis skills on newborns.

Background: Examination maneuvers used to diagnose developmental hip dysplasia (DDH) translate poorly to video and written curricula. This poses a challenge to teaching the infant hip exam to orthopedic, family medicine, and pediatric trainees. This work investigated the impact of the MiHip simulation-based training program on residents' knowledge, confidence, and exam skills in the simulated setting, and translation of these skills to the clinical setting.

Higher Rate of Reinterventions After Transfemoral Carotid Artery Stenting in Symptomatic Patients: A Retrospective Stroke Center's Cohort Study between 2015-2024.

Introduction: Carotid artery stenosis is a significant contributor to ischemic strokes, and its surgical management includes carotid artery endarterectomy (CEA), transfemoral carotid artery stenting (TF-CAS), and trans carotid artery revascularization (TCAR). CEA has traditionally been preferred, but TF-CAS and TCAR are also excellent alternative options if the anatomy of the vessels allows them. This study reports our short- and mid-term outcomes after carotid artery revascularization in symptomatic patients at a stroke center.

Endovascular therapy is effective in patients with post-thrombotic syndrome based on anatomical classification: a multi-center experience.

Objective: In this study, we aimed to retrospectively review patients with post-thrombotic syndrome (PTS) and investigate its long-term outcomes, and a novel classification were presented across multiple institutions.

Methods: We retrospectively evaluated patients with PTS who underwent endovascular therapy at two institutions between January 2018 and September 2023. Baseline patient demographics, lesion characteristics, and in-hospital and follow-up outcomes were collected and analyzed retrospectively.

Correlation Between Osteal Kneading and Carotid Artery Atherosclerotic Plaque.

Background: Adjacent bony structures may directly rub the carotid artery during swallowing or head and/neck movement. Long-term repeated stimulation might be considered to be a potential risk factor for carotid atherosclerotic plaque formation, development, and hazard. we defined the process as "Osteal Kneading".

Development of a clinical and ultrasonic parameter-based nomogram model to predict restenosis after superficial femoral artery stenting.

Objectives: Prediction of in-stent restenosis (ISR) is clinically important for patients with peripheral artery disease in their superficial femoral arteries (SFA) who have been treated with stenting. The aim of this study was to construct and validate a predictive model for ISR after SFA stenting based on a series of clinical and ultrasonic parameters.

Methods: This retrospective study included 381 patients who were treated with self-expanding bare nitinol stents in their SFA at our hospital between January 1, 2018, and January 1, 2022.

Current trends and evidence on post-TEVAR aorto-pulmonary and -bronchial fistula: a narrative review.

Introduction: Aorto-bronchial or aorto-pulmonary fistulas (ABPF) are a rare but life-threatening complication following thoracic endovascular aortic repair (TEVAR). This narrative review aims to provide an overview of the current trends and available evidence on ABPF following TEVAR, evaluating risk factors, diagnostic approaches, and possible preventive and therapeutic strategies.

Methods: Relevant publications on post-TEVAR ABPF were selected through a literature search on PubMed.