α-Tocopherol Long-Chain Metabolite α-T-13'-COOH Exhibits Biphasic Effects on Cell Viability, Induces ROS-Dependent DNA Damage, and Modulates Redox Status in Murine RAW264.7 Macrophages.

Scope: The α-tocopherol long-chain metabolite α-tocopherol-13'-hydroxy-chromanol (α-T-13'-COOH) is a proposed regulatory intermediate of endogenous vitamin E metabolism. Effects of α-T-13'-COOH on cell viability and adaptive stress response are not well understood. The present study aims to investigate the concentration-dependent effects of α-T-13'-COOH on cellular redox homeostasis, genotoxicity, and cytotoxicity in murine RAW264.

Sustainable Land Use Strengthens Microbial and Herbivore Controls in Soil Food Webs in Current and Future Climates.

Climate change and land-use intensification are threatening soil communities and ecosystem functions. Understanding the combined effects of climate change and land use is crucial for predicting future impacts on soil biodiversity and ecosystem functioning in agroecosystems. Here, we used a field experiment to quantify the combined effects of climate change (warming and altered precipitation patterns) and land use (agricultural type and management intensity) on soil food webs across nematodes, micro-, and macroarthropods.

Complex chromosomal rearrangements in female carriers experiencing recurrent pregnancy loss or poor obstetric history and literature review.

Purpose: Complex chromosomal rearrangements (CCRs) often remain unidentified as they are rarely observed in the general population. Females with CCRs are generally recognized on the identification of an affected child with multiple congenital anomalies (MCA) or having a history of repeated pregnancy loss/bad obstetric history (RPL/BOH). In contrast, males with CCRs are diagnosed primarily due to infertility.

Motion-induced blindness shows spatial anisotropies in conscious perception.

Polar angle asymmetries (PAAs), the differences in perceptual experiences and performance across different regions of the visual field are present in various paradigms and tasks of visual perception. Currently, research in this area is sparse, particularly regarding the influence of PAAs during perceptual illusions, highlighting a gap in visual cognition studies. We aim to fill this gap by measuring PAAs across the visual field during an illusion applied to test conscious vision widely.

Inter-chromosomal contacts demarcate genome topology along a spatial gradient.

Non-homologous chromosomal contacts (NHCCs) between different chromosomes participate considerably in gene and genome regulation. Due to analytical challenges, NHCCs are currently considered as singular, stochastic events, and their extent and fundamental principles across cell types remain controversial. We develop a supervised and unsupervised learning algorithm, termed Signature, to call NHCCs in Hi-C datasets to advance our understanding of genome topology.

Syntheses and structures of dimesitylphosphinite complexes of alkali metals and their catalytic activity in hydrophosphorylation reactions.

Metalation of dimesitylphosphane oxide, MesP(O)H (1), with alkali metal reagents (BuLi, NaH, and A(hmds); A = K, Rb, and Cs) in THF yields the corresponding dimesitylphosphinites of lithium (2-thf), sodium (3-thf), potassium (4-thf), rubidium (5-thf), and caesium (6). Their molecular structures exhibit a broad and fascinating variety. Dinuclear compounds 2-thf, 3-thf, and 5-thf have central four-membered AO rings, whereas the potassium congener crystallises as a tetranuclear complex with an inner AO heterocubane cage.

Rfam 15: RNA families database in 2025.

The Rfam database, a widely used repository of non-coding RNA families, has undergone significant updates in release 15.0. This paper introduces major improvements, including the expansion of Rfamseq to 26 106 genomes, a 76% increase, incorporating the latest UniProt reference proteomes and additional viral genomes.

Repetitive DNAs: the 'invisible' regulators of insect adaptation and speciation.

Like other eukaryotes, insect genomes contain a large portion of repetitive sequences, particularly transposable elements and satellite DNAs. This review highlights key studies on repetitive DNAs and examines their structural, functional, and evolutionary impact on insect genomes. Repetitive sequences promote genetic diversification through mutations and large-scale rearrangements, playing a crucial role in shaping genomic architecture, aiding organismal adaptation, and driving speciation.

Catching the π-Stacks: Prediction of Aggregate Structures of Porphyrin.

π-π interactions decisively shape the supramolecular structure and functionality of π-conjugated molecular semiconductor materials. Despite the customizable molecular building blocks, predicting their supramolecular structure remains a challenge. Traditionally, force field methods have been used due to the complexity of these structures, but advances in computational power have enabled ab initio approaches such as density functional theory (DFT).

Reliability of sEMG data of back muscles during static submaximal loading situations - Values and pitfalls.

Alterations of trunk muscle activity can be objectified using surface electromyography (sEMG). But for diagnostic purposes reliable reference values are needed. Therefore, the present study evaluated sEMG measurements of representative back muscles concerning their reliability.

Standardizing Nutritional Care for Cancer Patients: Implementation and Evaluation of a Malnutrition Risk Screening.

Introduction: Cancer-related malnutrition is a highly prevalent, yet often overlooked concern in clinical practice. Although cancer-related management guidelines recommend standardized nutritional care, its implementation is scarce. The aim of this study was to investigate the prevalence of malnutrition and the medical need for nutrition counseling in cancer patients employing a novel standardized nutritional management program (containing malnutrition risk screening, nutritional assessment, and counseling).

Organ-specific electrophile responsivity mapping in live C. elegans.

Proximity labeling technologies are limited to indexing localized protein residents. Such data-although valuable-cannot inform on small-molecule responsivity of local residents. We here bridge this gap by demonstrating in live C.

Image quality of virtual monochromatic and material density iodine images for evaluation of head and neck neoplasms using deep learning-based CT image reconstruction - A retrospective observational study.

Purpose: To compare the quality of deep learning image reconstructed (DLIR) virtual monochromatic images (VMI) and material density (MD) iodine images from dual-energy computed tomography (DECT) for the evaluation of head and neck neoplasms with CT scans from a conventional single-energy protocol.

Method: A total of 294 head and neck CT scans (98 VMIs operated at 60 keV, 102 MD iodine images, and 94 images from a 120 kVp single-energy CT (SECT) protocol) were retrospectively evaluated. VMIs and MD iodine images were generated using the Gemstone Spectral Imaging (GSI) mode using DLIR and metal artifact reduction (MAR) algorithms.

An Increase in the Rigidity of the Environment Favors MLCT over the MC State in [Ru(bpy)(Nicotine)](Cl): A Case Study of Photolabile Ligands.

Ru(II)-complexes with photolabile ligands find a wide range of applications, e.g., in drug release and in the design of light-responsive interfaces.

Web-based telemedicine approach for treatment of post-COVID-19 in Thuringia (WATCH).

Objective: After infection with SARS-CoV-2, a substantial proportion of patients develop long-lasting sequelae. These sequelae include fatigue (potentially as severe as that seen in ME/CFS cases), cognitive dysfunction, and psychiatric symptoms. Because the pathophysiology of these sequelae remains unclear, existing therapeutic concepts address the symptoms through pacing strategies, cognitive training, and psychological therapy.

Editorial: Protein post-translational modifications in the nervous system: from development to disease and ageing.

PTMs are crucial for biological processes contributing to healthy organ function. Protein post-translational modifications (PTMs), such as phosphorylation (P), acetylation (Ac), SUMOylation (SUMO), S-nitrosylation (Nitro), ubiquitination (Ub) and glycosylation (Glyco), affect a wide range of cellular and biological functions as depicted in this cartoon. Perturbations lead to severe consequences for the normal function of the brain and other organs, such as muscle.

Leveraging Organ-on-Chip Models to Investigate Host-Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease.

Inflammatory bowel disease (IBD) is an idiopathic gastrointestinal disease with drastically increasing incidence rates. Due to its multifactorial etiology, a precise investigation of the pathogenesis is extremely difficult. Although reductionist cell culture models and more complex disease models in animals have clarified the understanding of individual disease mechanisms and contributing factors of IBD in the past, it remains challenging to bridge research and clinical practice.

Meadows or asphalt road - Does momentary affective well-being vary with immediate physical environment? Results from a geographic ecological momentary assessment study in three metropolitan areas in Germany.

We investigated the association between the immediate physical environment of individuals and individual fluctuations of momentary affective well-being in everyday life with a focus on medium sized metropolitan areas in Europe representing a typical living environment of a large proportion of the world's population. The sample comprised 365 individuals (54.8 % female) with participants ranging from 14.