Quantitative photoacoustic spectral transformations in theranostic solid lipid nanoparticles labelled with increasing concentrations of a photoacoustic NIR BODIPY.

Solid lipid nanoparticles (SLNs) have shown great capabilities for drug delivery and are therefore attractive theranostic candidates when labelled with an imaging contrast agent. This work aims to create the first SLNs labelled for photoacoustic (PA) imaging by encapsulating a specially designed and near-infrared absorbing BODIPY dye (BY-aniline-Palm) into SLNs of dexamethasone palmitate. A one-pot formulation protocol enabled us to replace the prodrug with the BY-aniline-Palm label in various proportions up to 100%.

Quantitative, precise and multi-wavelength evaluation of the light-to-heat conversion efficiency for nanoparticular photothermal agents with calibrated photoacoustic spectroscopy.

Biomedical photothermal therapy with optical nanoparticles is based on the conversion of optical energy into heat through three steps: optical absorption, thermal conversion of the absorbed energy and heat transfer to the surrounding medium. The light-to-heat conversion efficiency (LHCE) has become one of the main metrics to quantitatively characterize the last two steps and evaluate the merit of nanoparticules for photothermal therapy. The estimation of the LHCE is mostly performed by monitoring the temperature evolution of a solution under laser irradiation.