Objectivity of two methods of differentiating fibre types and repeatability of measurements by application of the TEMA image analysis system.

The objectivity of two of the most widely used methods for differentiation of fibre types, i.e. 1) the myosin ATP-ase method (Brooke and Kaiser, 1970a,b) and 2) the combined method, by which the myosin ATP-ase reaction is used to differentiate between fast and slow twitch fibres and NADH-tetrazolium reductase activity is used to identify the subgroups of fast twitch fibres (Ashmore and Doerr, 1970, Peter et al.

Review and an experimental study on the physiological and quantitative aspects of gluconeogenesis in lactating ruminants.

A short review describes quantitative aspects of glucose metabolism in lactating ruminants, including the contribution of different substrates to glucose synthesis as affected by nutritional and hormonal regulation. Gluconeogenesis from propionate (the major gluconeogenic substrate in the fed state) is mainly regulated by feed intake and increased propionate availability and is less affected by insulin and glucagon. Quantitative estimates of amino acid contribution to glucose synthesis are highly variable (from 2 to 40% of glucose flux), but no conclusive data exist by which this large variation can be explained.

Effect of bovine GH and ovariectomy on mammary tissue sensitivity to IGF-I in prepubertal heifers.

Sixteen prepubertal Holstein Friesian heifers were used to study the effect of long-term administration of bovine GH (bGH; 15 mg/day for 15 weeks) and ovariectomy (at 2.5 months of age) on GH and IGF-I receptors in mammary and liver parenchymal tissue. Heifers were slaughtered on the day after the last injection of bGH or excipient.

Effect of exogenous bovine growth hormone and ovariectomy on prepubertal mammary growth, serum hormones and acute in-vitro proliferative response of mammary explants from Holstein heifers.

Sixteen prepubertal Holstein Friesian heifers were used to study the effect of long-term administration of bovine GH (bGH) on mammary development in intact and ovariectomized heifers. Eight heifers were ovariectomized at 2.5 months of age.