Chinese expert consensus on the diagnosis of osteoporosis by imaging and bone mineral density.

With an aging society, osteoporosis is one of the most common diseases threatening the health of China's elderly population and is an issue that is raising increasing concern. Osteoporosis is characterized by bone loss and increased susceptibility to fragility fractures. Various imaging modalities such as X-ray, CT, MRI and nuclear medicine along with assessment of bone mineral density (BMD) play an important role in its diagnosis and management, and the treatment requires multidisciplinary teamwork.

[Screening of Differential Expression Proteins in Rat Brain Tissues after DAI by iTRAQ-LC-MS/MS].

Objectives: To screen for the differential expression proteins in brain tissues of SD rat after diffuse axonal injury （DAI） by isobaric tag for relative and absolute quantification-liquid chromatograph-mass spectrometer/mass spectrometer （iTRAQ-LC-MS/MS）, and to explore potential biomarkers available for the diagnosis of DAI.

Methods: Animal models of DAI were established with the Marmarou method as reference, and the subjects were divided into blank control group （=4）, sham strike group （=4） and fatal strike group （=4）, respectively. The proteins in rat brain tissues were detected by iTRAQ-LC-MS/MS, and bioinformatics analysis and verification were performed on the results and screened for the differential expression proteins.

[Changes of HbCO in the Blood of Rats with Different CO Concentration and Inhalation Time].

Objectives: To explore the change rules of behavioral characteristics, survival time and saturation of carboxyhemoglobin （HbCO） in different CO concentration to provide experimental basis for the cases of CO poisoning death in forensic practice.

Methods: Total 160 SD rats were randomly divided into four groups. CO with the concentration of 1 250 mg/m³, 3 750 mg/m³, 6 250 mg/m³ were continuously and respectively replenished in a self-made toxicant exposure equipment until rats died from poisoning.

[Monitoring precursor chemicals through enantiomer profiling of methamphetamine].

Objective: The enantiomer profiling of methamphetamine is can be utilized for regulation of the precursors, investigatin of the drug case.

Methods: In the present study, we investigated on the enantiomer of 114 crystalline methamphetamine samples seized in Beijing, Tianjin, Shijiazhuang and Chengdu. The methamphetamine samples were derivatized with (S)-(+)-a-methoxya-(trifluoromethyl)phenylacetyl chloride ((S)-(+)-MTPACl), and the derivatives were analyzed by GC-MS in selected ion monitoring (SIM) mode.

[Determination of the synthetic route based on impurity profiling of methamphetamine].

Objective: Impurity profiling of seized methamphetamine may play an important role in the determination of the synthetic method employed and the criminal investigations of drug traffic routes.

Methods: A sample of methamphetamine was dissolved in buffer solution. Impurities were extracted with ethyl acetate containing four internal standards (n-decane, n-pentadecane, n-eicosane and n-octacosane) and analyzed by GC-MS.

[Study on the linked samples of methamphetamine identified by the impurity profile].

Objective: To compare and classify the samples of MA crystals by using impurity profile to offer methods for identification of linked samples.

Methods: 50 mg samples of MA were dissolved in 1 ml of buffer solution (pH 7.0, four parts of 0.

Identification of impurities and statistical classification of methamphetamine hydrochloride drugs seized in China.

A total of 48 methamphetamine hydrochloride samples from eight seizures were analyzed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography with a flame ionization detector (GC-FID). Major impurities detected include 1,2-dimethyl-3-phenylaziridine, ephedrine/pseudoephedrine, 1,3-dimethyl-2-phenylnaphthalene, 1-benzyl-3-methylnaphthalene. These data are suggestive of ephedrine/pseudoephedrine as the main precursor of the methamphetamine hydrochloride samples seized during 2006-2007.

[Study on the optimization methods of common-batch identification of amphetamine samples].

The essay introduced the technology of amphetamine identification and its optimization method. Impurity profiling of amphetamine was analyzed by GC-MS. Identification of common-batch amphetamine samples could be successfully finished by the data transition and pre-treating of the peak areas.

[Determination of three phenoxyalkanoic acid herbicides in blood using gas chromatography coupled with solid-phase extraction and derivatization].

A method for the determination of three phenoxyalkanoic acid herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 2-(2,4-dichlorophenoxy)-propanoic acid (2,4-DP), and 4-chloro-2-methylphenoxy-acetic acid (MCPA), in blood was developed. The blood sample was diluted with 0.1 mol/L hydrochloric acid, and extracted by solid-phase extraction using porous resin GDX401 as adsorbent and ethyl ether as eluent.

[Polymorphisms analysis of mitochondrial DNA in coding area].

Objective: To analyze mitochondrial DNA (mtDNA) polymorphisms in coding area and provide a theoretical basis for applying in forensic science.

Methods: The primers of 8162F/8483R and 13070F/13299R were designed according to the Anderson's sequence. Using PCR-sequencing method to detect polymorphisms of mtDNA nt8162-8483 and nt13070-13299.