Development of an Enzyme-Linked Immunosorbent Assay Based on a Monoclonal Antibody for the Rapid Detection of Citrinin in Wine.

The ingestion of food contaminated with citrinin (CIT) poses a variety of health risks to humans and animals. The immunogens (CIT-COOH-BSA, CIT-H-BSA) and detection antigen (CIT-COOH-OVA, CIT-H-OVA) were synthesised using the active ester method (-COOH) and formaldehyde addition method (-H). A hybridoma cell line (3G5) that secretes anti-CIT monoclonal antibodies (mAbs) was screened via CIT-H-BSA immunisation of mice, cell fusion, and ELISA screening technology.

BenchAMRking: a Galaxy-based platform for illustrating the major issues associated with current antimicrobial resistance (AMR) gene prediction workflows.

Background: The Joint Programming Initiative on Antimicrobial Resistance (JPIAMR) networks 'Seq4AMR' and 'B2B2B AMR Dx' were established to promote collaboration between microbial whole genome sequencing (WGS) and antimicrobial resistance (AMR) stakeholders. A key topic discussed was the frequent variability in results obtained between different microbial WGS-related AMR gene prediction workflows. Further, comparative benchmarking studies are difficult to perform due to differences in AMR gene prediction accuracy and a lack of agreement in the naming of AMR genes (semantic conformity) for the results obtained.

Evaluating food contact surface and its influence on restaurant health ratings.

Controlling and maintaining the cleanliness of the working environment in restaurants is crucial. The aim of this study was to determine the level of contamination on food contact surfaces in restaurants. A total of 28 restaurants were surveyed, and 500 samples were taken from food contact surfaces that appeared clean and ready for use.

Unexpectedly life-threatening meal: Contamination by Bongkrekic acid in Taiwan.

Objective: Bongkrekic acid (BKA) is a life-threatening toxin linked to foodborne illnesses. Herein, we report two fatalities among 33 patients who ingested BKA in March 2024, East Taipei, Taiwan.

Case Report: Our first case involved a 40-year-old female who presented with nausea, vomiting, and diarrhea after consuming wet rice noodles.

Health Inspections of Restaurant Establishments in the Attica Region, Greece. Non-compliance Data Within the Food Hygiene Sector.

Background: Ensuring food safety is a fundamental priority for public health. The catering sector has become prominent as a convenient and cost-effective method of food supply worldwide. Adherence to proper food hygiene practices is crucial for preventing foodborne diseases.

Template-assembly activation of primer exchange reaction for on-site and sensitive detection of copper ions in blood.

The sensitive and accurate detection of copper ions is crucial for public health, medical research, and environmental monitoring. In this study, we developed a sensor based on template-assembly activation of the primer exchange reaction (PER) for the on-site detection of copper ions in blood. Copper ions triggered the assembly of two template fragments into a hairpin structure via a click-chemistry reaction, activating the PER.

Chitosan-based nanocomposite films incorporated with AgNPs/porphyrinic MOFs for killing pathogenic bacteria.

In this work, a nanocomposite film, designated as CS/PA, was fabricated by integrating chitosan (CS), porphyrinic porous coordination network (PCN), and silver nanoparticles (AgNPs). PCN modified AgNPs was denoted as PCN-AgNPs (PA). The synthesis of PA was verified through transmission electron microscope, Zeta potential, hydrated particle size, element mapping.

Design and preparation of novel magnetic covalent organic framework for the simultaneous preconcentration and sensitive determination of six aflatoxins in food samples.

An innovative core-shell covalent organic framework (COF), FeO@COF (ETTBA-ND), was synthesized through a facile and energy-efficient method. This adsorbent facilitated magnetic solid phase extraction (MSPE) of six AFs prior to LC-MS/MS analysis, achieving one-step purification and enrichment in food matrices. The successful synthesis of the adsorbent was confirmed using various techniques, with adsorption capacities ranging from 46.

Spatial metabolomics and feature-based molecular networking to unveiling in-situ quality markers landscape and reflecting geographic origins of pomegranate seeds.

Pomegranate seeds, a by-product of pomegranate processing, are gaining attention in food industries due to their high antioxidant activity. However, the lack of quality markers reflecting activity and spatial characteristics limits their utilization and product stability. In this research, a selective and sensitive method integrating ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry with feature-based molecular networking, and desorption electrospray ionization-mass spectrometry imaging developed to identify components and locate in-situ images of quality markers via spatial metabolomics analysis.

Processing and inspection of high-pressure microfluidics systems: A review.

In the field of microfluidics, high-pressure microfluidics technology, which utilizes high driving pressure for microfluidic analysis, is an evolving technology. This technology combines microfluidics and pressurization, where the flow of fluid is controlled by means of high-pressure-driven devices greater than 10 MPa. This paper first reviews the existing high-pressure microfluidics systems and describes their components and applications.

First molecular characterisation of in a pig carcass condemned during routine meat inspection in Poland.

Introduction: This article presents the fourth detection of macroscopic cystic lesions due to sarcocystosis in domestic pigs during routine meat inspection worldwide, and the first molecular detection of in a domestic pig in Poland. Pigs can become intermediate hosts for by accidental ingestion of oocysts or sporocysts present in food or water contaminated by the faeces of canids (definitive hosts).

Material And Methods: The affected swine showed no clinical symptoms such as weight loss, dermatitis or dyspnoea suggesting sarcocystosis.

A Real-World Pharmacovigilance Study of FDA Adverse Event Reporting System Events for Obeticholic Acid.

Background And Objectives: Based on the Adverse Event Reporting System (FAERS) data from the US FDA, this study mined the adverse drug reactions of obeticholic acid (OCA) in the real world and provided reference for clinical safe drug use.

Methods: Adverse event reports for OCA from the second quarter of 2016 to the third quarter of 2023 were extracted. The analysis for adverse reaction signal detection was conducted using reporting odds ratio, proportional reporting ratio, Bayesian confidence propagation neural network, and multi-item gamma Poisson shrinker methods.

Complete genome sequence of a strain associated with a bovine tuberculosis outbreak on a cattle farm in Saskatchewan, Canada.

Bovine tuberculosis is an important zoonotic infectious disease that presents a risk to human health, livestock, and wildlife. We report the complete genome sequence of a new strain that caused a bovine tuberculosis outbreak on a cattle farm in Saskatchewan, Canada, in 2023.

Phenotypic Differences Between the Epidemic Strains of Vesicular Stomatitis Virus Serotype Indiana 98COE and IN0919WYB2 Using an In-Vivo Pig () Model.

During the past 25 years, vesicular stomatitis virus (VSV) has produced multiple outbreaks in the US, resulting in the emergence of different viral lineages. Currently, very little is known about the pathogenesis of many of these lineages, thus limiting our understanding of the potential biological factors favoring each lineage in these outbreaks. In this study, we aimed to determine the potential phenotypic differences between two VSV Indiana (VSIV) serotype epidemic strains using a pig model.

Evolutionary Relationships of Unclassified Coronaviruses in Canadian Bat Species.

Bats are recognized as natural reservoirs for an array of diverse viruses, particularly coronaviruses, which have been linked to major human diseases like SARS-CoV and MERS-CoV. These viruses are believed to have originated in bats, highlighting their role in virus ecology and evolution. Our study focuses on the molecular characterization of bat-derived coronaviruses (CoVs) in Canada.

Protein Expression Platforms and the Challenges of Viral Antigen Production.

Several protein expression platforms exist for a wide variety of biopharmaceutical needs. A substantial proportion of research and development into protein expression platforms and their optimization since the mid-1900s is a result of the production of viral antigens for use in subunit vaccine research. This review discusses the seven most popular forms of expression systems used in the past decade-bacterial, insect, mammalian, yeast, algal, plant and cell-free systems-in terms of advantages, uses and limitations for viral antigen production in the context of subunit vaccine research.

Analysis of Toxic Element Levels and Health Risks in Different Soybean Species (, , , ).

Soybeans are a widely consumed legume, essential in Western diets and especially prominent in vegan and vegetarian nutrition. However, environmental contamination from anthropogenic sources, such as industrial emissions, wastewater, and pesticide use, has led to the accumulation of non-essential and toxic elements in legumes, potentially impacting human health. This study quantified the levels of 11 potential toxic elements (Al, B, Ba, Cd, Co, Cr, Li, Ni, Pb, Sr, V) in 90 samples of four soybean species (, , , ) using inductively coupled plasma optical emission spectrometry (ICP-OES).

The Effects of Ultrasound on the Rehydration of Konjac Glucomannan/Soy Protein Isolate Gel and Simulation of Gas-Liquid Interface Evolution During the Rehydration Process.

Soy protein isolate (SPI) possesses potential gelling properties, making it suitable for gel-based applications. However, the gel network stability and mechanical properties of SPI are relatively poor and can be improved through modifications or by combining it with other polymers, such as Konjac Glucomannan (KGM). Combining SPI with KGM can overcome the poor gel network stability and mechanical properties of SPI, but it reduces the water-absorbing capacity of the gel network after drying, which affects the quality characteristics of plant-based protein rehydrated foods and limits the economic feasibility of soy protein foods.

Loop-Mediated Isothermal Amplification-Based Workflow for the Detection and Serotyping of spp. in Environmental Poultry Flock Samples.

spp. is one of the most important foodborne pathogens worldwide. Given the fact that poultry and poultry products are the main source of human infection, Salmonella control in these farms is of utmost importance.

Research on Pork Cut and Freshness Determination Method Based on Computer Vision.

With the increasing importance of meat quality inspection, traditional manual evaluation methods face challenges in terms of efficiency and accuracy. To improve the precision and efficiency of pork quality assessment, an automated detection method based on computer vision technology is proposed for evaluating different parts and freshness of pork. First, high-resolution cameras were used to capture image data of Jinfen white pigs, covering three pork cuts-hind leg, loin, and belly-across three different collection times.

DNAzyme-mediated isothermal catalytic hairpin assembly for rapid and enzyme-free amplified detection of lead(Ⅱ) ion.

The detection of heavy metal ions, particularly lead (Pb²⁺), in environmental samples is crucial for public health and safety. Current nucleic acid signal amplification methods for Pb²⁺ detection often rely on biological enzymes and are limited in applicability due to high costs, prolonged detection times, and nonspecific adsorption. In this study, we introduce an enzyme-free, DNAzyme-mediated isothermal catalytic hairpin assembly (DMICHA) assay, which combines a DNAzyme-based Pb²⁺ recognition module with a signal amplification process utilizing isothermal catalytic hairpin assembly (CHA).

Meals, Mealtimes and moments for learning: Assessment of quality in Early Childhood Education and Care services.

Objective: Early education and care (ECEC) is part of the everyday life of most children in developed economies presenting exceptional opportunity to support nutrition and ongoing food preferences. Yet, the degree to which such opportunity is captured in policy-driven assessment and quality ratings of ECEC services is unknown.

Design: Abductive thematic analysis was conducted, guided by key domains of knowledge in nutrition literature and examining identified themes within these domains.

An E-Delphi study to facilitate animal welfare assessment in Italian zoos and aquaria.

Ensuring animal welfare is a key aspect of animal management in zoological facilities and aquaria, representing a pivotal facet of their mission. Italy currently lacks a comprehensive and valuable assessment methodology for evaluating the welfare of captive animals. To address this gap, the present study aimed to identify the most important criteria that should be considered in the welfare management and assessment of animals housed in Italian zoos and aquaria.

Assessment of HACCP plans and Colombian regulations in municipal cattle slaughterhouses for the assurance of standardised food safety and quality management systems.

Colombia has strengthened regulations to ensure standardized food safety and quality in bovine slaughterhouses. To this end, the Ministry of Health and Social Protection designed to empower INVIMA, as the governing and coordinating authority for the operation of the Official Meat Inspection, Surveillance and Control System. This regulation aims to evaluate the technical conditions of infrastructures, slaughter processes and quality and safety assurance systems.