1,870 results match your criteria: "First Military Medical University.[Affiliation]"
Zhong Yao Cai
November 2004
Institute of Pharmaceutical Sciences, First Military Medical University, Guangzhou.
This is the first report to purify a new peptide SV-PP-1 from Agkistrodon blomhoffii Bioe. SV-PP-1 has molecular weight of 1234.616 Da and can inhibit on platelet aggregation.
View Article and Find Full Text PDFWorld J Gastroenterol
March 2005
Chinese PLA Institute of Digestive Disease, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China.
Aim: To ascertain the molecule mechanism of nuclear factor-kappaB (NF-kappaB) inhibitor curcumin preventive and therapeutic effects in rats' colitis induced by trinitrobenzene sulfonic acid (TNBS).
Methods: Sixty rats with TNBS-induced colitis were treated with 2.0% curcumin in the diet.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
April 2005
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou, Guangdong,510515 P. R. China.
Objective: To explore the association between chimerism, minimal residual disease (MRD) and relapse after sex-mismatched allogeneic hematopoietic stem cell transplantation (allo-HSCT) for leukemia.
Methods: Fifty-seven patients with leukemia received allogeneic hematopoietic stem cell grafts from HLA-matched or partially matched, but sex-mismatched donors. Chimeric status and MRD were detected by dual-color interphase fluorescence in situ hybridization (I-FISH) using X/Y sex chromosome centromere DNA probe and bcr/abl dual fusion DNA probe, respectively, at different time points after transplantation.
Chin Med J (Engl)
March 2005
Institute for Neuroscience of the First Military Medical University, Zhujiang Hospital, Guangzhou 510282, China.
Background: A new brain region, the marginal division (MrD), was discovered at the caudal margin of the neostriatum. The MrD was shown to be involved in learning and memory in the rat. The aim of this study was to investigate the expression of the immediate-early genes c-fos and c-jun in the MrD of the striatum during learning and memory processes in the rat, immunocytochemical and Western blot methods were used to examine Y-maze trained rats.
View Article and Find Full Text PDFInterv Neuroradiol
March 2005
Department of Neurosurgery, Zhujiang Hospital, The First Military Medical University, Guangzhou; China -
We report a patient with a wide-necked aneurysm arising at the bifurcation of the right internal carotid artery and the persistent primitive trigeminal artery (PPTA) treated successfully by Matrix detachable coil occlusion and assisted by a Neuroform intracranial stent. First, a Neuroform self-expanding intracranial stent was delivered via a 5-F Guider Softtip XP and placed as desired, then the aneurysm dome was embolized with two Matrix detachable coils through the interstices of the stent. The aneurysm was 80% occluded angiographically and the parent artery was patent.
View Article and Find Full Text PDFZhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
February 2005
Department of Orthopaedic Surgery, Nanfang Hospital, the First Military Medical University, Guangzhou Guangdong, 510515, PR China.
Objective: To study the mechanism of ectopic osteogenesis of nacre/Polylactic acid (N/P) artificial bone combined with allogenic osteoblasts, and to explore the possibility as a scaffold material of bone tissue engineering.
Methods: The allogenic osteoblasts seeded onto N/P artificial bone were co-cultured in vivo 1 week. The N/P artificial bone with allogenic osteoblasts were implanted subcutaneously into the left back sites of the New Zealand white rabbits in the experimental group and the simple N/P artificial bone into the right ones in the control group.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
February 2005
Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
The aim was to determine RHD zygosity, further to investigate genetic structure of RHD gene, and to predict hemolytic disease of newborn (HDN). The upstream box, downstream box, and hybrid box of RHD gene were determined by PCR-SSP with 4 primers under the same conditions. The results showed that only hybrid box could be determined in RHD(-)/RHD(-) homozygosity.
View Article and Find Full Text PDFZhonghua Fu Chan Ke Za Zhi
December 2004
Department of Obstetrics and Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective: To investigate the clinical effect and safety of heparin in treating fetal growth restriction (FGR).
Methods: A total of 107 women diagnosed with FGR were randomized into three groups. Group 1 (standard heparin group, n = 37) received 50 - 75 mg standard heparin added to 500 ml 5% glucose sodium chloride, infused intravenously in 6 - 8 hours.
Zhonghua Shao Shang Za Zhi
October 2004
Department of Burns, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, P.R. China.
Objective: To investigate the effects of burn serum on nuclear translocation of monocytic NF-kappaB heterodimers p50/p65 and the degradation of inhibiting kappaB (IkappaBalpha), so as to further explore the role of burn serum on the activation of monocytes.
Methods: Peripheral blood monocytes (PBMCs) isolated from healthy volunteers were employed as the target cells. The cells were stimulated by the serum from healthy volunteers and burn patients, and by burn serum together with pyrrolidine dithiocarbamate (PDTC).
Genet Test
April 2005
Department of Medical Genetics, First Military Medical University, Guangzhou 510515, Guangdong Province, PR China.
beta-thalassemia is one of the most common inherited diseases with incidence varying between 3% and 10% in the high-prevalence regions of South China. The molecular defects are mostly due to single-nucleotide substitutions, minor insertions, and deletions in the beta-globin gene. Large-scale population genetic screening combined with prenatal diagnosis is necessary for the effective prevention of this disease.
View Article and Find Full Text PDFMicrofilaments, composed of global actins, involve in many important physiological activities, such as the maintenance of cell shape and cell migration. Actin-related protein 2/3 ( Arp2/3) complex plays a key role in microfilament formation. Actin-related protein 2/3 ( Arp2/3 ) complex which is composed of seven different subunits is subjected to the modulation of many nucleation promoting factors (NPFs) and cooperates with these factors to regulate the actin nucleation.
View Article and Find Full Text PDFCancer Lett
March 2005
Department of Pathology, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
Although it is fairly well accepted that pulmonary tuberculosis is a major risk factor of lung cancer, the exact molecular mechanisms involved in its tumorigenesis are unclear. For this purpose, we have examined the relationship between Mycobacterium tuberculosis (M-TB) infection and FHIT gene alteration in lung cancer. Tumors with M-TB infection had a slightly higher abnormal FHIT protein expression compared with tumors without M-TB infection, although not statistically significant (Fisher's exact test, P=0.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
March 2004
First Military Medical University, Guangzhou 510515, China.
Objective: To analyze the chemical constituents of supercritical carbon dioxide extraction products from Rhodiola tibetica.
Method: The chemical constituents were separated and identified by gas chromatography-mass spectrometry (GC-MS).
Result: 26 Components were identified, and their relative contents were determined by normalization method of area.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
January 2005
Department of Clinical Laboratory, Southern Medical University (the First Military Medical University), Guangzhou Guangdong 510515, P R China.
Objective: To investigate the current situation and developing trend of antithrombotic function study of endothelium in vascular tissue engineering.
Methods: The effect of several elements on the antithrombotic ability of endothelium, including the source of endothelium, the characteristic of the matrix materials, the cell culture methods, and the endothelium's gene modification were analyzed.
Results: The normal antithrombotic function of tissue engineered vascular relied on the source of endothelium, gene modification of seeding cells, the cell culture methods in vitro, and the characteristic of the scaffolds.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
January 2005
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University (the First Military Medical University), Guangzhou Guangdong 510515, P R China.
Objective: To replace dysfunctional Fas gene and reconstruct the blocked Fas signal by using two kinds of prepared recombinant Adenovirus which have human Fas gene .
Methods: After the keloids derived from fibroblasts were infected by the Adenovirus, the expressions of Fas protein before the exposure and after the exposure was compared. Then the function of the newly produced Fas protein was detected.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
January 2005
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University (the First Military Medical University), Guangzhou Guangdong 510515, PR China.
Objective: To investigate the relationship between p53 codon 72 polymorphism and susceptibility to keloid.
Methods: The p53 genotypes were detected by polymerase chain reaction-reverse dot blot (PCR-RDB) and DNA direct sequencing among 15 healthy controls and 15 patients with keloid.
Results: The frequency of the Pro allele (P = 0.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
January 2005
Southern Medical University (the First Military Medical University), Guangzhou Guangdong 510515, P R China.
Objective: To build animal models of keloid by method of tissue engineering and to discuss the feasibility of using it in clinical and lab researches.
Methods: Fibroblasts(FB) were isolated from keloids and cultured. The seventh and eighth generation of the cultured FBs were inoculated into the copolymers of polylactic acid and polyglycolic PLGA.
Zhonghua Er Bi Yan Hou Ke Za Zhi
October 2004
Centre of Orthopaedics and Traumatology of PLA, Nan Fang Hospital, The First Military Medical University, Guangzhou 510515, China.
Objective: To explore the method of fabricating tissue engineered laryngeal cartilage.
Methods: The rib and articular cartilage of infant New Zealand white rabbits were harvested in sterile condition. The chondrocytes were separated by collagenase digestion and cultured in vitro for 3 passage.
Di Yi Jun Yi Da Xue Xue Bao
January 2005
Institute of Clinical Anatomy, First Military Medical University, Southern Medical University, Guangzhou 510515, China.
Objective: To study the effect of FK506 in promoting apoptosis of peripheral blood-derived macrophages activated by homogenate of allogenic nerve tissues.
Methods: Homogenate of the allogenic nerve tissues was prepared using the sciatic nerve and injected in one-month-old SD rats, from which the macrophages activated by the homogenate were collected from the abdominal cavity and cultured in vitro. The cells were divided into 4 groups according to different concentrations of FK506 for treatment, namely 0 (group A, control group), 0.
Zhong Yao Cai
August 2004
Nanfang Hospital, First Military Medical University, Guangzhou.
Objective: To observe whether rat vascular smooth muscle cells (VSMCs) proliferation induced by native low-density lipoprotein (LDL) is affected by flavone from leaves of Diospyros kaki in vitro.
Methods: Rat aortic VSMCs were cultured in vitro and treated with LDL and flavone from leaves of Diospyros kaki, respectively, and were observed in comparison with the control group. The ratio of cell proliferation was determined by non-radioactive MTS/PES assay.
World J Gastroenterol
February 2005
Department of Pathology, First Military Medical University, Guangzhou 510515, Guangdong Province, China.
Aim: To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis.
Methods: Expressions of Tiam1 gene in 8 colorectal carcinoma cell lines were detected by reverse transcriptase-polymerase chain reaction. In vitro invasiveness was determined by means of Matrigel invasion assay.
Zhong Xi Yi Jie He Xue Bao
January 2005
Department of Pharmacology, First Military Medical University, Guangzhou, Guangdong Province 510515, China. jpx
Objective: To observe the effects of ginkgolide (GL) on the cerebral blood flow in dogs.
Methods: Dogs anesthetized with sodium pentobarbital were randomly divided into 5 groups, with 4 dogs in each group. Ginkgolide of 4.
World J Gastroenterol
January 2005
Department of Gastroenterology, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, Guangdong Province, China.
Aim: To investigate the relationship between gastric dysmotility, gastrointestinal hormone abnormalities, and neuroendocrine cells in gastrointestinal mucosa in patients with functional dyspepsia (FD).
Methods: Gastric emptying was assessed with solid radiopaque markers in 54 FD patients, and the patients were divided into two groups according to the results, one with delayed gastric emptying and the other with normal gastric emptying. Seventeen healthy volunteers acted as normal controls.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
December 2004
Department of Medical Genetics, First Military Medical University, Guangzhou, Guangdong, 510515 P. R. China.
Objective: To develop a primer-extension in combination with denaturing high-performance liquid chromatography (PE-DHPLC)-based assay for prenatal diagnosis of the five most common beta-thalassemia mutations in Chinese.
Methods: The human beta-globin gene fragment was amplified by PCR, followed by a multiple PE reaction specific for each five mutations. Then the PE product mixtures were separated for genotyping of beta-globin gene mutations using fully-denaturing DHPLC analysis.
Zhonghua Yi Xue Za Zhi
October 2004
Division of Nephrology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective: To investigate the expression of receptor for advanced glycation end products (RAGE) in chronic renal failure (CRF) and its role in monocyte-mediated inflammation associated with chronic renal failure (CRF).
Methods: Peripheral monocytes (PMC) were isolated from 96 non-diabetic patients with varying severity of CRF. RAGE expression on monocytes was quantitated by flow cytometry.