[Applications of AFM in mechanism study of Alzheimer's disease].

Atomic force microscope (AFM) has become a well-established technique for biological sample imaging under physiological conditions. The unique capacity to produce nanometer scale resolution images with simple specimen preparation enables AFM to serve as a powerful tool in neurobiology research. Extensive studies suggest that the conversion of beta-amyloid (Abeta) peptide from soluble forms into fibrillar structure is a key factor in the pathogenesis of Alzheimer's disease (AD).

Using targeted magnetic arsenic trioxide nanoparticles for osteosarcoma treatment.

The present study was to investigate the therapeutic efficacy of the magnetic arsenic trioxide (ATO) nanoparticles against osteosarcoma in vivo tumor models. ATO was incorporated in the magnetic nanoparticles and encapsulated by poly lactic acid. Human MG-63 osteosarcoma cells were injected subcutaneously into the nude mice.

[The tone of sympathetic and expression of beta1 receptor in the dogs with sustained atrial fibrillation].

Objective: To detect the influence of sustained atrial fibrillation on autonomic nerve.

Methods: 7 healthy mongrel dogs were paced at 600/min. ter sustained reversing into sinus rhythm.

The effect of streptomycin on stretch-induced electrophysiological changes of isolated acute myocardial infarcted hearts in rats.

Aims: To explore whether the stretch of ischaemic myocardium could modulate the electrophysiological characteristics, especially repolarization via mechanoelectric feedback (MEF), as well as the effect of streptomycin (SM) on these changes. Methods Thirty-six wistar rats were randomly divided into four groups: control group (n = 9), SM group (n = 9), myocardial infarction (MI) group (n = 9), and MI + SM group (n = 9). After perfused on Langendorff, the isolated hearts were stretched for 5s by a ballon inflation of 0.

Various tolerances to arsenic trioxide between human cortical neurons and leukemic cells.

Arsenic trioxide (As2O3) is very effective for treatment of acute promyelocytic leukaemia (APL) but little can pass through the blood-brain-barrier (BBB), which limits its use in the prevention and treatment of central nervous system leukaemia (CNSL). Before creating a non-invasive method to help As2O3 's access, the safe and effective therapeutic concentration of As2O3 in the CNS ought to be known. The changes of apoptosis biomarkers, [Ca2+]i and PKC activity of both leukaemia cells and human cortical neurons, were monitored before and after being treated with As2O3 in vitro with laser confocal microscopy and Western blot.

[Expression of BDNF and trk B in the cochleas of the aged rats].

Objective: To explore the correlation between the expression of brain derived neurotrophic factor (BDNF) and presbycusis in the cochleae of the aged rats.

Method: The mRNA expression of BDNF and presbycusis in the cochleas of the aged rats was quantitated by real time PCR.

Result: The expression of BDNF in the cochleas of the aged rats with presbycusis was obviously less than that in the control group.

[Clinicopathologic study of ischemic intestinal disease due to mesenteric venous lesions].

Objective: To study the clinical and pathologic features of ischemic intestinal disease due to mesenteric phlebitis.

Method: The clinical and pathologic features of the mesenteric venous lesions in 3 patients of ischemic intestinal disease admitted during the period from 2003 to 2004 were studied.

Results: All 3 patients had a clinical history of acute abdominal pain accompanying with a diffuse peritonitis.

Effects of arsenic trioxide administration styles on leukocytosis.

Objective: To study the effects of constantly slow intravenous arsenic trioxide (As2O3) infusion regimen on decreasing leukocytosis in vivo and in vitro.

Methods: Three kinds of leukemia cells, NB4, K562, and acute promyelocytic leukemia (APL) cells, were cultured in the media with constant concentration and varying concentrations of As2O3 respectively for 24 hours. Seventy-five patients were enrolled in two groups randomly.

Clinicopathological and immunohistochemical analysis of gastrointestinal stromal tumor.

Aim: To investigate the clinicopathological features of gastrointestinal stromal tumor (GIST) and to study the reference indexes for malignancy.

Methods: Fifty-two cases of primary GIST were distinguished from a group of gastrointestinal mesenchymal tumors using a panel of antibodies such as CD117 and CD34 by immunohistochemical SP method. Their biological behaviors were analyzed using the expression of p21WAF1 and Bax in 52 cases of GIST.

Serum concentrations of IL-2 and TNF-alpha in patients with painful bone metastases: correlation with responses to 89SrCl2 therapy.

Unlabelled: We have used 89SrCl2 for the palliative treatment of painful bone metastases from various malignant diseases. We studied the correlation between serum interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) levels and the response to 89SrCl2 therapy.

Methods: Forty-two patients (24 men and 18 women) were treated intravenously with 89SrCl2 at a dose of 148 MBq (4 mCi).

A case of overlapping Bickerstaff's brainstem encephalitis and Guillain-Barré syndrome.

Objective: There is no report on Bickerstaff's brainstem encephalitis (BBE) patients in China. We here report the first case of BBE in China.

Methods: Clinical features, results of electromyography, electroencephalography (EEG), magnetic resonance imaging (MRI) and cerebrospinal fluid (CSF) examination were studied to clarify the characteristics of this syndrome.

[Study on the effect of spinal neural progenitor transplantation on treating brachial].

Objective: To explore the effect of spinal neural progenitor transplantation to the cervical spinal on The brachial plexus treating brachial plexus injury with the reimplantation of the avulsed spinal roots.

Methods: avulsed injury model was made on 54 rats and they were evenly divided into 3 groups: fresh group, chronic group, control group. The spinal neural progenitor was cultured and identified.

[The diagnostic value of telomerase activity in differentiating benign and malignant ascitic fluid].

Objective: To investigate the changes of telomerase activity in benign and malignant ascites fluid.

Methods: The technique of telomerase TRAP-PCR-ELISA was employed to detect telomerase activity in ascites cells from 60 patients with benign or malignant ascites fluid, the cytological diagnosis and total tumor marks (carcinoembryonic antigen, et al.) were compared.

[Expression of amyloid beta protein and amyloid precursor protein after focal brain ischemia in human].

Objective: To observe the expression of amyloid beta precursor protein and amyloid beta protein in neurons of hippocampal CA1 region after brain ischemia in human.

Methods: Neuronal damage was examined by using HE staining, and expression of APP and Abeta(1-40) was determined by immunohistochemistry in the hippocampal CA1 region of the brain specimens of 43 patients who died 2 h-6 h, 7 h-24 h, 25 h-48 h, 49 h-72 h, 73 h-96 h, 97 h-144 h, or 145 h-168 h after cerebral ischemia and in 2 specimens of patients who died of other diseases as control group.

Results: The expression of Abeta(1-40) was 25.

[Effects of combination therapy with angiotensin I-converting enzyme inhibitor and angiotensin 1 receptor antagonist on ventricular remodeling and expression of endothelial nitric oxide synthase].

Objective: To evaluate the effects of ACEI, AT1 receptor antagonism, and combination of these two drugs on ventricular remodeling, and the vary of eNOS in rats with myocardial infarction (MI).

Methods: Eighty-six rats were randomly divided into four groups at twenty-four hours after MI, and treated for 2 weeks and 6 weeks. (1) MI-placebo (n = 22); (2) MI-Fosinopril (n = 20, 10 mg.

[Effects of protein tyrosine kinase, protein tyrosine phosphatase and protein kinase C on the apoptosis of arsenic trioxide treated NB4 cells and human cortex neurons].

Objective: To investigate the effects of protein tyrosine kinase (PTK), protein tyrosine phosphatase (PTP) and protein kinase C (PKC) on apoptosis and observe the changes of cytosolic calcium ([Ca(2+)]i) of arsenic trioxide (As2O3) treated human leukemia cells NB4 and cortex neurons.

Methods: [Ca(2+)]i of NB4 cells and cortex neurons was probed with Fluo-3/AM, its changes were assayed with laser confocal microscopy in real-time after As2O3 treatment at different concentrations, the effects of PTK and PTP and the activation of PKC on these changes with confocal microscopy and phosphorus radioisotope assay. DNA ladders of NB4 cells and cortex neurons after exposed to As2O3 were observed.

Effect of arsenic trioxide on human hepatocarcinoma in nude mice.

Aim: To study the effect of arsenic trioxide (As(2)0(3)) on human hepatoma cell line BEL-7402 in vivo.

Methods: Human hepatoma cell line BEL-7402 cultured in vitro was inoculated into nude mice and arsenic trioxide, 5-Fu and saline were injected into abdominal cavity of the nude mice respectively. The volumes of tumor and general conditions of the nude mice and structural changes of the liver and kidney were observed.

Correlation between neuronal injury and Caspase-3 after focal ischemia in human hippocampus.

Background: Cerebral ischemia is a significant clinical problem, and cerebral ischemia usually causes neuron injury such as apoptosis in various brain areas, including hippocampus. Cysteinyl aspartate-specific protease (Caspases) are fundamental factors of apoptotic mechanism. Caspase-3 inhibitors show effect in attenuating brain injury after ischemia.

[Association between subacute combined degeneration, vitamin B12 deficiency and megaloblastic anemia].

Objective: To investigate the association between subacute combined degeneration (SCD) and vitamin B(12) (VB(12)) deficiency and megaloblastic anemia (MA).

Methods: The serum level of VB(12), severity of anemia and lesions in CNS were analysed in 36 cases diagnosed as SCD. In addition, MRI neuro-electrophysiologic examination and CSF myelin basic protein (MBP) concentration were monitored dynamically.