Association of CD14 and TLR4 with LPS-stimulated human normal skin fibroblasts in immunophenotype changes and secretion of TGF-β1 and IFN-γ.

Objectives: We attempted to explore the association of CD14 and TLR4 with LPS-stimulated human normal skin fibroblasts in immunophenotype changes and secretion of TGF-β1 and IFN-γ, and to expand the current knowledge of the mechanisms that underlie LPS-induced scar formation.

Methods: We randomized the human normal skin fibroblasts cultured in vitro into four groups. The expression profile of immune phenotypes was determined by immunohistochemical staining.

Role of CD14 and TLR4 in type I, type III collagen expression, synthesis and secretion in LPS-induced normal human skin fibroblasts.

Objectives: The primary aim of this study was to investigate the role of CD14 and TLR4 in type I, type III collagen expression, synthesis and secretion in LPS-induced normal human skin fibroblasts. The secondary aim was to provide theoretical basis for the molecular mechanisms of scar formation induced by LPS.

Methods: The normal skin fibroblasts cultured in vitro were randomly divided into four groups: 0.

Effects of CD14 and TLR4 on LPS-mediated normal human skin fibroblast proliferation.

Background: This study was designed to elucidate the effects of CD14 and TLR4 on the proliferation of LPS-mediated normal human skin fibroblasts and to gain new insights into the mechanisms underlying LPS-induced scar formation.

Methods: Cell morphology and density were observed using an inverted microscope. Proliferation curves were described by MTT.

Identification of collaboration patterns of dysfunctional pathways in breast cancer.

Breast cancer (BC) is the most common malignancy among women. We aimed to illuminate the molecular dysfunctional mechanisms of BC progression. The mRNA expression profile of BC GSE15852 was downloaded from Gene Expression Omnibus database, including 43 normal samples and 43 cancer samples.