[Study on combined therapy of hyperlipidemic severe acute pancreatitis].

Objective: To investigate the principle and measures of combined treatment of the patients with hyperlipidemic severe acute pancreatitis (HL-SAP).

Methods: The clinical data of 54 patients with HL-SAP including two phases from January 1996 to December 2000 and from January 2001 to August 2006 were analyzed retrospectively. In the first phase, 25 patients were performed by routine methods to decrease triglyceride, or additional operative treatments.

[Calpain I inhibition prevents pacing-induced structural remodeling for atrial fibrillation in canine].

Objective: To study the relation of the structural remodeling processes and activation of calpain I.

Methods: Fifteen dogs were randomly divided into three groups. The dogs in pacing group (n=5) and inhibitor group (n=5) were subjected to 3 weeks of rapid atrial pacing at 600 beats/min, control dogs (n=5) were in sham-operated group.

Protective effect and mechanism of stronger neo-minophagen C against fulminant hepatic failure.

Aim: To investigate the protective effect of stronger neo-minophafen C (SNMC) on fulminant hepatic failure (FHF) and its underlying mechanism.

Methods: A mouse model of FHF was established by intraperitoneal injection of galactosamine (D-Gal N) and lipopolysaccharide (LPS). The survival rate, liver function, inflammatory factor and liver pathological change were obtained with and without SNMC treatment.

Ten-year experience with living related donated splenic transplantation for the treatment of hemophilia A.

Aim: Six cases of hemophilia A treated with living related donated splenic transplantation (LRDST) were performed over 10 years.

Methods: We reviewed the six consecutive cases of LRDST from 1992 to 2002. Three patients received whole spleen allografts and the other three, partial spleen allografts.

[Effect of local mild hypothermia on expression of aquaporin-4 following intracerebral hemorrhage in rats].

Objective: To examined the effect of local mild hypothermia on the expression of aquaporin-4 (AQP-4) following intracerebral hemorrhage (ICH) in rats and clarified the mechanism of hypothermia on brain edema formation following ICH.

Methods: Two hundreds and forty male Wistar rats were randomly divided into two groups: the intracerebral hemorrhage (ICH) group, in which autologous arterial blood were stereotaxically injected into right caudate nucleus; the local mild hypothermia (ICH + H) group, in which the rats were given 4 h local mild hypothermia after the injection of blood. Each group was divided into 6 subgroups: control, 6 h, 24 h, 72 h, 5 d and 7 d after operation; Brain water content was determined by dry-wet weight method and the permeability of BBB was measured by Evans-Blue extravasation.

Microencapsulating hepatocytes.

Background: Because standardization of the cell microencapsulation procedure has not yet been achieved, we performed hepatocyte microencapsulation using alginate (ALG)-poly-l-lysine (PLL)-ALG (APA) polymer.

Methods: Hepatocytes were microencapsulated using a binozzle air-jet droplet generator. Our study aims were to: (1) clarify how ALG concentration affects the quality of ALG beads; (2) determine how the PLL concentration affects the quality of microcapsules (MCs); (3) ascertain the influence of liquefaction time by sodium citrate (SC) on the quality of the MCs; (4) and clarify how temperature and solution pH, respectively, affect the viability of the hepatocytes inside the MCs.

[Relationship between expression of Smad and ventricular remodeling after myocardial infarction in rats].

Objective: To investigate the relationship between expression of Smad3, Smad7 and ventricular remodeling in rats after myocardial infarction.

Methods: Myocardial infarction was induced by left anterior descending coronary artery ligation in rats (n = 11) and sham-operated rats were used as control (n = 10). The rats were sacrificed 8 weeks later.

Microencapsulated hepatocytes and islets as in vivo bioartificial liver support system.

Aim: To confirm the xenotransplantation of microencapsulated hepatocytes and islets as a temporary bioartificial liver support system for mice with acute liver failure (ALF).

Methods: Mice were rendered ALF by a single intra-peritoneal injection of D-galactosamine (D-gal) and their tail blood was sampled to examine differences in blood ALT, albumin (ALB), total bilirubin (TB) and glucose (GLU) between 4 experimental groups. Rat hepatocytes and islets were collected and microencapsulated referring to both Sun's and Fritschy's methods.

The potential role of IGF-I receptor mRNA in rats with diabetic retinopathy.

Objective: To evaluate the potential role of insulin-like growth factor-1 receptor mRNA (IGF-IR mRNA) in the onset and development of retinopathy in diabetic rats.

Methods: A diabetic model was duplicated in Wistar rats. The early changes in the retina were examined using light and transmission electron microscopy.

[Primary clinical study on self-setting calcium phosphate cement in bone defect repair of extremities].

Objective: To investigate the clinical application of self-setting calcium phosphate cement (CPC) in bone defect repair of extremities.

Methods: From May 1998 to January 2000, 32 cases of bone defect, in 36 sites, were repaired and reviewed, aged from 4 to 59 years old (24.7 years old on average), with bone defect 2 to 125 cm2 in size (13.

A large scale epidemiological survey of Graves' disease in Daqing area.

Objective: To study the incidence of Graves' disease and the associated factors.

Methods: Totally 100,123 people aged 15 to 79 years in Daqing area were surveyed epidemiologically. The investigation was conducted for two years.