Intravital assessment of angioarchitecture in rat hepatocellular nodules using fluorescent microscopy.

Background: To prospectively evaluate the stepwise changes that occur in intra-nodular microvessels and microcirculation during the carcinogenesis process of hepatocellular nodules by using fluorescent microscopy, and to compare these with pathological changes.

Methods: Forty-five 10-week-old male Wistar rats received drinking water containing N-nitrosomorpholine at 10 mg/100 mL for 18weeks to develop multiple hepatocellular carcinomas (HCC) and dysplastic nodules (DN) in the liver; meanwhile, the non-lesion liver tissues become fibrotic. The microvascular morphological change and hemodynamic change of two lesion areas (HCC or DN) and one non-lesion area in each rat were observed with fluorescent microscope.

[The development and collagen ingredients analysis of submandibular gland acellular matrix].

Purpose: To develop submandibular gland acellular matrix and observe its structure and analyze its histological ingredients.

Methods: The fresh submandibular glands of SD mice were cell-extracted by chemical detergent and histological ingredients were analyzed by immunohistochemistry.

Results: Submandibular gland cells disappeared, matrix ingredients appeared network structure under microscopy.

[The development and ultrastructure study of acellular submandibular gland scaffold].

Purpose: To develop nature acellular submandibular gland scaffold materials and observe its ultrastructures.

Methods: The fresh submandibular glands of SD rats were cell-extracted by chemical detergent and observed with transmission and scanning electron microscope.

Results: Submandibular gland cells were dissolved and destroyed under transmission electron microscope.