Factors affecting pregnancy outcome resulting from assisted reproductive technology (ART).

Objective: A retrospective, observational study of pregnancy outcome was performed on variables maintained in an ART database to determine factors that might affect miscarriage rate in pregnancies resulting from assisted reproduction technologies (ART).

Methods: Previously infertile couples, where conception was achieved after ART, were included. Seven hundred and ninety-four consecutive clinical pregnancies, diagnosed by ultrasound documentation of the gestation sac in the first trimester were divided into 2 groups: 'miscarriage' and 'term birth'.

[The secretion and receptor gene of insulin-like growth factor-I quantitative expression in ovarian stroma in polycystic ovarian syndrome patients].

Objective: To explore varying different degrees of the action of insulin-like growth factor-I (IGF-I) and its receptor gene quantitative expression in the ovarian stroma of women with obese and nonobese polycystic ovarian syndrome (PCOS).

Method: The expression of IGF-I receptor gene in the ovarian stroma from 35 cases of PCOS (obese: 15 cases; nonobese: 20 cases) and 20 cases of control were quantitatively determined by reverse transcript polymerase chain reaction (RT-PCR) combined with beta(2) micro globlin (beta(2) MG) gene as inner-control to amplificate sychronously. The grey value ratio of them were determined.