Expression of nestin and glial-derived neurotrophic factor in human endogenous neural stem cells following ischemia.

Objectives: We investigated the relationship between glial cell line-derived neurotrophic factor (GDNF), nestin, and the activation of endogenous neural stem cells (NSCs) following cerebral infarction in humans.

Methods: Post-mortem brain specimens from patients who died following cerebral infarction were examined.

Results: Compared with the controls, the number of nestin-positive cells was increased at 4·5-10 hours in the subgranular zone of the hippocampus dentate gyrus and at 24-72 hours in the subventricular zone, reaching maximum levels at 120-144 hours.

[Influences of bFGF and EGF on the proliferation and differentiation of endogenous neural stem cells after cerebral infarction in human].

Objective: To investigate the relationship of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) and proliferation of endogenous neural stem cells (NSCs) after human cerebral infarction.

Methods: Paraffin-embedded brain tissues of 22 human fatal cases of CI from the brain tissues around subventricular zone and subgranular layer zone were stained with HE and immunohistochemistry stain. The endogenous neural stem cells were marked by nestin.

[Expressions of heme oxygenase-1 and apoptosis-modulating proteins in peri-hematoma cortex after intracerebral hemorrhage in human being].

Objective: To observe the expression of heme oxygenase-1 (HO-1) and Bcl-2, an apoptosis-modulating protein, in the neurons surrounding the hematoma in human being.

Methods: Specimens of cerebral cortex tissue 1 - 3 cm around the hemorrhagic focus with the size of 2.0 cm x 1.