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Effect of ionizing radiation on transcription of colorectal cancer MDR1 gene of HCT-8 cells.
Asian Pac J Trop Med
May 2013
Gastroenterology Department, Fifth Hospital of Sun Yat-sen University Zhuhai 51900, China.
Objective: To discuss effect of ionizing radiation on transcription of colorectal cancer multidrug resistance (MDR) 1 gene of HCT-8 cells.
Methods: Total RNA was extracted by guanidine thiocyanate one-step method. Northern blot was applied to detect transcription level of MDR1 gene.