Metabolomic Analysis and Computational Biology Reveal the Potential Metabolic Pathways for Improvement of Fucoxanthin and Eicosapentaenoic Acid (EPA) Biosynthesis in Phaeodactylum tricornutum.

This work aims to provide a basis for the enhancement of fucoxanthin (FCX) and eicosapentaenoic acid (EPA) biosynthesis in the microalga Phaeodactylum tricornutum using metabolomics and computational biology. To achieve this, both targeted (UHPLC and GC-FID) and untargeted (FTIR and NMR) analyses were conducted throughout various stages of cell cultivation. Targeted analyses revealed that EPA concentrations peaked at the end of the logarithmic growth phase, while fucoxanthin levels remained consistent from the onset of this phase through to the stationary phase.

Transforming sludge into energy: impact of blend percentage on energy recovery potential and environmental benefits of co-combustion of wastewater sludge and coal in Brazil.

Proper waste management and sustainable energy production are crucial for human development. For this purpose, this study evaluates the impact of blending percentage on energy recovery potential and environmental benefits of co-combustion of wastewater sludge and Brazilian low-rank coal. The sludge and coal were characterised in terms of their potential as fuel and co-combustion tests were carried out in a pilot-scale bubbling fluidised bed focused on the influence of the percentage of sludge mixture on the behaviour of co-combustion with coal in terms of flue gas composition and fluidised bed temperature stability.

Global research interest and publication trends on guided surgery in implant dentistry: A metrics-based analysis.

Statement Of Problem: Digital surgical guides improve precision by detailing the direction, position, and angle of implants, which reduces surgery time and complications. A bibliometric analysis of guided surgery in implant dentistry is lacking.

Purpose: The aim of this metrics-based analysis was to analyze the trends and key characteristics of articles related to guided surgery in implant dentistry.

Gene transcription in the oyster Crassostrea gigas exposed to environmental concentrations of the UV filter Benzophenone-3.

Personal care products (PCPs), such as sunscreens, are usually found in various aquatic ecosystems at low concentrations (ng l to µg l). However, there is limited information regarding their effects on marine bivalves. Therefore, the aim of this study was to evaluate the sublethal effects of environmental concentrations (1 and 100 µg l) of benzophenone-3 (BP-3) in Crassostrea gigas oysters after 1 and 7 days of exposure.

Disinfection Strategies for Poly(methyl methacrylate): Method Sequence, Solution Concentration, and Intraoral Temperature on Antimicrobial Activity.

This study aimed to evaluate the antimicrobial effectiveness of different disinfection protocols for dentures by combining methods, varying intervention sequences, sodium hypochlorite (NaOCl) concentrations (0.1% and 0.25%), and post-exposure to intraoral temperature.

Association of systemic inflammation and long-term dysfunction in COVID-19 patients: A prospective cohort.

COVID-19 has significant long-term impacts, including a chronic syndrome known as long-COVID, characterized by persistent symptoms post-recovery. The inflammatory response during acute infection is hypothesized to influence long-term outcomes. This study aimed to identify inflammatory biomarkers predictive of functional outcomes one year after hospital discharge.

Macauba (Acrocomia ssp.) fruits: A comprehensive review of nutritional and phytochemical profiles, health benefits, and sustainable oil production.

Macauba is an underexplored palm with significant potential for food-grade vegetable oil production. Its fruits yield two distinct sources of oil, the pulp and the kernel, each with its unique composition, emerging as a potential vegetable oil source with high competitiveness with well-established conventional oil sources. Besides the oil, macauba fruits are rich in essential nutrients, including proteins, minerals, vitamins, dietary fiber, and phytochemicals, with outstanding health benefits.

A Narrative Review on the Neuroprotective Potential of Brown Macroalgae in Alzheimer's Disease.

Systematic Alzheimer's disease (AD) is a neurodegenerative disease increasingly prevalent in the aging population. AD is characterized by pathological features such as -amyloid (A) plaque accumulation, tau neurofibrillary tangles formation, oxidative stress, an impaired cholinergic system, and neuroinflammation. Many therapeutic drugs have been developed to slow the progression of AD by targeting these pathological mechanisms.

Do instrument kinematics and the apical preparation limit influence canal disinfection and bacterial extrusion?

This in vitro research assessed the influence of the instrument kinematics (rotary and reciprocating) and the apical preparation limit on the root canal disinfection and apical bacterial extrusion. After 21 days of Enterococcus faecalis biofilm formation in 72 mesial root canals of mandibular molars, the root canals were distributed into 2 groups (n = 36) according to the systems used for preparation: ProDesign S and Reciproc. The groups were redistributed according to the limit of apical preparation (n = 11): (a) 1 mm up to the apical foramen (TL-1); (b) at the apical foramen (TL = 0); (c) 1 mm beyond the apical foramen (TL + 1).

Hepatocyte nuclear factor 4-α is necessary for high fat diet-induced pancreatic β-cell mass expansion and metabolic compensations.

Aims: This study investigates the role of Hepatocyte Nuclear Factor 4α (HNF4α) in the adaptation of pancreatic β-cells to an HFD-induced obesogenic environment, focusing on β cell mass expansion and metabolic adaptations.

Main Methods: We utilized an HNF4α knockout (KO) mouse model, with CRE-recombinase enzyme activation confirmed through tamoxifen administration. KO and Control (CTL) mice were fed an HFD for 20 weeks.

Protective effects of different exercise modalities on oxidative stress in animal models of high intraocular pressure and diabetes.

High intraocular pressure (HIOP) and high glucose levels are associated with oxidative stress. Although physical exercise protects against oxidative damage, its specific impact on eye health remains unclear. Thus, this study aimed to assess the impact of physical exercise on the oxidative status of whole eyes in male Swiss mice subjected to HIOP model and cafeteria diet (CD).

Water pollution caused by ash from grassland fires alters the molecular, biochemical, and morphological biomarkers of non-biting midge larvae.

The frequency and intensity of wildfires have been increasing in many parts of the world, which may result in biodiversity loss. Wildfires can devastate plant communities, generating toxic ash that pollutes watercourses through runoff. However, our understanding of the effects of ash exposure on aquatic biodiversity is still limited.

Electrophysiological findings of brainstem auditory evoked potentials in infants with down syndrome: A systematic review and meta-analysis.

Objective: to summarize the evidence on the electrophysiological findings in the auditory brainstem response (ABR) in infants with DS.

Material And Methods: This is a systematic review study, whose protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO CRD42023424139) and conducted in accordance with the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Observational studies were included: cross-sectional and cohort studies that performed ABR evaluation in infants with DS up to two years of age, which had their results compared with normal infants, of the same age group.

Mechanistic Insights into Chloride-Dependent Uncaging Reaction of Propargyl and Allene-Protected Substrates by Pd-Complexes.

This study investigates the effect of chloride levels on the mode of action of palladium complexes for the activation of propargyl- and allene-protected fluorophores and chemotherapeutic drugs through uncaging reactions. Four Pd(II) complexes were synthesized and characterized using various spectroscopic techniques to confirm their structure and electronic properties. Kinetic studies and density functional theory calculations revealed that chloride ions in phosphate buffered saline (PBS) significantly enhance catalytic efficiency, particularly for allenyl-protected substrates compared to propargylic counterparts.

Antioxidant properties of phenolic extracts of murtilla pomace: First report on the importance of soluble and insoluble-bound compounds.

Some native Chilean berries, including murtilla, have gained attention for their high phenolic content which renders them attractive for the beverage industry. However, phenolic-rich by-products are generated during the production of murtilla juice (murtilla pomace), and there has been no scientific consideration of this by-product as a source of different forms of phenolic compounds. The aim of this study was thus to obtain phenolic extracts from the soluble fraction (free, esterified, and etherified) of murtilla pomace as well from the counterpart that contains insoluble-bound compounds, and evaluate their antioxidant properties.

Seamless Transition to Post-Quantum TLS 1.3: A Hybrid Approach Using Identity-Based Encryption.

We propose a novel solution to streamline the migration of existing Transport Layer Security (TLS) protocol implementations to a post-quantum Key Encapsulation Mechanism for Transport Layer Security (KEMTLS). By leveraging Identity-Based Encryption (IBE), our solution minimizes the necessary modifications to the surrounding infrastructure, enabling the reuse of existing keys and certificates. We provide a proof-of-concept implementation and performance analysis, demonstrating the practical feasibility and effectiveness of our proposed approach.

Evaluation of Admixture Silane Added into Cementitious Pastes.

This manuscript evaluated the performance of silanes in cementitious matrices in the partial replacement of superplasticizers by silanes. For this, pastes with a water/cement ratio of 0.186 were produced and the superplasticizer admixture based on polycarboxylate esters was partially substituted by three types of silanes-vinyltriethoxysilane silanes (VTES), n-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTMS), and methacryloxypropyltrimethox-ysilane (MCPTMS)-in two substitutions levels (20% and 40%), and then tested in Portland cement pastes.

Immediate effects of Kinesio Taping ® on respiratory parameters in healthy individuals.

Background: Kinesio taping® (KT) could facilitate respiratory muscle contraction, improving breathing. Therefore, this study aimed to analyze the immediate effects of KT application on thoracoabdominal mobility, vital capacity, and respiratory muscle strength of healthy young individuals.

Methods: This clinical trial included 19 healthy individuals, measuring their maximum inspiratory and expiratory pressure, vital capacity, and thoracoabdominal mobility using cirtometry.

Impact of pre-fermentative maceration techniques on the chemical characteristics, phenolic composition, in vitro bioaccessibility, and biological activities of alcoholic and acetic fermented products from jaboticaba (Plinia trunciflora).

The jaboticaba is a fruit with a highly promising chemical profile for producing fermented products with biological activity. In this context, evaluating pre-fermentative maceration techniques in jaboticaba fermentations is essential to optimize the extraction of phenolic compounds and other metabolites of interest, imparting antioxidant characteristics to the product and offering potential health benefits. This study evaluated the impact of two pre-fermentative maceration techniques on the phenolic composition, bioaccessibility, and biological activities (α-amylase, α-glucosidase, and ACE inhibition) of alcoholic and acetic fermented products from jaboticaba.

Electrospun Poly(vinylidene fluoride) Nanocomposites with Ionic Liquid Functionalized Graphene Nanoplatelets by a Noncovalent Method for Piezoresistive Pressure Sensor Applications.

Piezoresistive pressure sensors have been prepared by the electrospinning of poly(vinylidene fluoride) (PVDF) containing graphene nanoplatelets (GNP) functionalized using 1-butyl-3-methylimidazolium trifluoromethanesulfonate (BMIM(OTf)) ionic liquid (IL). Optical microscopy demonstrated that the functionalized GNP powder presented particles with a smaller lateral size. The obtained mats were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, differential scanning calorimetry, electrical resistivity using two and four probes, and electromechanical testing with up to 32 load-unload cycles.

Organic vs. inorganic nitrates: Metabolic and vascular outcomes in STZ-induced diabetes in mice.

Background: Diabetic animals often display dysregulated nitric oxide (NO) metabolism, contributing to vascular dysfunction. This study evaluates the metabolic and vascular effects of organic nitrate isosorbide mononitrate (ISMN) versus inorganic sodium nitrate (NaNO3) in mice with type 1 diabetes mellitus (T1DM) induced by streptozotocin (STZ).

Experimental Approach: T1DM was induced in male C57Bl6 mice with STZ ip and confirmed by fasting glucose.

Temporal Analysis and Spatial Distribution of Mortality Related to Head and Neck Cancer in the State of Santa Catarina, Brazil.

To analyze mortality from head and neck cancer (HNC) in the State of Santa Catarina from 1979 to 2023. Ecological study of data available in the Mortality Atlas of the National Cancer Institute and in the Mortality Information System. Approximately 13,309 deaths due to HNC were recorded in the Mortality Atlas, considering the period from 1979 to 2021 and 11,027 deaths recorded in SIM in the period from 1996 to 2023.