Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap.

Background/objectives: Considering the large number of candidates in vaccine-testing studies against different pathogens and the amount of time spent in the preclinical and clinical trials, there is a pressing need to develop an improved in vivo system to quickly screen vaccine candidates. The model of a polyester-polyurethane sponge implant provides a rapid analysis of the specific stimulus-response, allowing the study of a compartmentalized microenvironment. The sponge implant's defined measurements were standardized as a compartment to assess the immune response triggered by the vaccinal antigen.

Susceptibility of Tambaqui () to Nile Tilapia-Derived and .

Nile tilapia () and tambaqui () are the two most produced freshwater fishes in Brazil. This study investigated the potential pathogenicity of and , previously isolated from diseased Nile tilapia, to tambaqui. Experimental infection trials were conducted in juvenile tambaqui at a dose of approximately 10 CFU fish, assessing clinical signs, mortality, bacterial recovery, and histopathological changes.

Infection in Wild Trahira () and Farmed Arapaima () in Brazil: An Interspecies Transmission in Aquatic Environments Shared with Nile Tilapia ().

is an important pathogen responsible for cases of high mortality in farmed and wild fish worldwide. In Brazil, this bacterium has been commonly associated with outbreaks in Nile tilapia farms, but other native fish species are also susceptible. Since floating cages are one of the most common culture systems used in the country, the close contact between farmed tilapia and native fish species presents a risk concerning the transmission of this pathogen.

Mitochondrial Genomes of Mammals from the Brazilian Cerrado and Phylogenetic Considerations for the Orders Artiodactyla, Carnivora, and Chiroptera (Chordata: Mammalia).

We assembled and annotated the complete mitochondrial genomes of (hoary fox), (bush dog), (white-lipped peccary), and (Brazilian free-tailed bat). The mitogenomes exhibited typical vertebrate structures, containing 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes, and a D-loop region. Phylogenetic reconstruction using the 13 protein-coding genes revealed robust relationships among species within Carnivora, Chiroptera, and Artiodactyla, corroborating previous studies.

Innovative Approaches in Knee Osteoarthritis Treatment: A Comprehensive Review of Bone Marrow-Derived Products.

Knee osteoarthritis (OA) is a chronic articular disease characterized by the progressive degeneration of cartilage and bone tissue, leading to the appearance of subchondral cysts, osteophyte formation, and synovial inflammation. Conventional treatments consist of non-steroidal anti-inflammatory drugs (NSAIDs), analgesics, and glucocorticoids. However, the prolonged use of these drugs causes adverse effects.

Humic Acid Derived from Vermicompost Inhibits Alveolar Bone Degradation and Protects Against Renal Injury in an Experimental Model of Periodontitis.

Periodontal disease (PD) leads to the destruction of supportive tissues through an inflammatory response induced by biofilm accumulation. This low-grade systemic inflammation from PD increases the risk of comorbidities. Among potential therapeutic agents for PD, humic acids (HAs) are notable for their anti-inflammatory and immunomodulatory properties.

COVID-19 Pandemic Impact on the Birth Weight of Children Born in a Brazilian Metropolis.

Objective: To assess the birth weight of newborns whose mothers gave birth during the COVID-19 pandemic.

Methods: A cross-sectional study based on data collected from medical records and through postnatal interviews to assess maternal and neonatal health outcomes (n = 470) during the pandemic. All participants were assisted in three Brazilian public hospitals in 2020.

Obesogenic Environment in a Minas Gerais State Metropolis, Brazil: Analysis of Crime Rates, Food Shops and Physical Activity Venues.

The aim of the present study is to identify obesogenic environment profiles to find the obesogenic environment pattern for Belo Horizonte City. The current research followed the ecological approach and was substantiated by data from food shops, public sports venues, crime rates (homicides and robberies) and the rate of accidents with pedestrians. Descriptive analyses and principal component analysis (PCA) were conducted in Stata software, version 14.

Leishmania sp. can affect the cardiovascular system of dogs - A systematic review of over 80 years.

Cardiovascular involvement in Leishmania sp. infections still requires further elucidation, with cutaneous and organic changes being more frequently observed. The aim of this study was to conduct a systematic review of cardiovascular involvement in dogs with canine visceral leishmaniasis, considering a publication span of over 80 years.

Mucosal-adapted bacteriophages as a preventive strategy for a lethal Pseudomonas aeruginosa challenge in mice.

Pseudomonas aeruginosa is an emergent threat due to the antimicrobial resistance crisis. Bacteriophages (phages) are promising agents for phage therapy approaches against P. aeruginosa.

Bioengineering chitosan-antibody/fluorescent quantum dot nanoconjugates for targeted immunotheranostics of non-hodgkin B-cell lymphomas.

B-cell non-Hodgkin lymphoma (NHL) is the most common hematologic malignancy, capable of invading the brain, meninges, and nerve roots of the brain and spine, leading to high lethality. Herein, we designed and developed novel nanostructures for the first time by biofunctionalizing chitosan with two specific antibodies (i.e.

Investigations into the growth and formation of biofilm by at temperatures encountered during infection.

The genus comprises unique atypical spirochete bacteria that includes the etiological agent of leptospirosis, a globally important zoonosis. Biofilms are microecosystems composed of microorganisms embedded in a self-produced matrix that offers protection against hostile factors. Leptospires form biofilms in rice fields and unsanitary urban areas, and while colonizing rodent kidneys.

Inter-island variability in trace elements and trophic ecology of Brown Booby (Sula leucogaster) in the South Atlantic.

This study investigates essential (Mg, Ca, Fe, Mn, Cu, Zn, Se, Ni) and non-essential (Li, Be, Cr, Rb, Sr, Cs, Cd, Sn, Ba, and Pb) element concentrations and stable isotope (δC, δN, δS) compositions in feathers of Brown Boobies (Sula leucogaster) from three distinct Atlantic islands: the Archipelagos of Saint Peter and Saint Paul (SPSP), Abrolhos, and Cagarras. We aimed to investigate the ecological and environmental factors influencing these seabird populations and assess potential variations in contaminant exposure and dietary habits based on location, sex, and maturity stages. Our finding revealed significant geographical differences in trace element concentrations.

Effectiveness of Enhanced Recovery After Surgery (ERAS) Protocol in Pancreatic Surgery - A Systematic review and Meta-Analysis of Randomized Controlled Trials.

Introduction: The Enhanced Recovery After Surgery (ERAS) protocol represents an advancement in perioperative care to reduce surgical stress and accelerate recovery. This meta-analysis evaluates the effectiveness of ERAS in pancreatic surgery.

Objective: To assess the impact of the ERAS protocol compared with conventional hospital care on postoperative outcomes, including length of hospital stay (LOS), hospital costs, readmission rates, and infection rates in patients undergoing pancreatic surgery.

Case Report: osteonecrosis of the maxilla and mandible related to trastuzumab, docetaxel and carboplatin.

We present a case of a patient with HER-2 positive breast cancer who developed extensive areas of osteonecrosis in the mandible and maxilla due to the combination of trastuzumab, carboplatin, and docetaxel. The pathogenesis is discussed based on the literature.

High-Energy Nutrition in Hospitalised Paediatric Patients With Congenital Heart Disease: A Systematic Review and Meta-Analysis.

Aims: Congenital heart disease (CHD) is a significant cause of growth failure and neonatal mortality worldwide, and requirement-focused nutritional management can deeply impact the prognosis. Despite multiple trials, there is no published meta-analysis on the impact of high-energy nutrition in this population.

Methods: We searched PubMed, Embase and Cochrane Central for RCTs comparing high-energy to standard formulas in hospitalised patients.

Equine endometrial bacteria inhibition by metabolite and extracellular vesicles of Lactobacillus acidophilus and lactiplantibacillus plantarum.

Endometritis is one of the main reproductive disorders in mares and due to the increasing prevalence of antibiotic resistance, the use of probiotics in the prevention and treatment of endometritis in mares has gained interest, given their potential to restore and maintain a healthy uterine microbiota. Therefore, the aim of this study was to evaluate the antimicrobial properties of total metabolites of Lactobacillus acidophilus (LA) and Lactiplantibacillus plantarum (LP) against common equine endometrial pathogenic bacteria in vitro (Acinetobacter baumannii, Escherichia coli (1), Escherichia coli (2), Escherichia coli (3), Escherichia coli (4), Enterobacter cloacae, Streptococcus equi, Staphylococcus warneri, Actinobacillus equi and Klebesiella pneumoniae), as well as to assess their low molecular weight metabolites (loM) and extracellular vesicle (EVs) inhibition capacity over a multidrug-resistant E. coli isolated from mares with clinical endometritis.

Inflammatory and Oxidative Patterns Regulated by Theracurmin Intake in an Experimental Model of Hypertrophic Training and Detraining.

Dietary supplements have improved performance and muscle hypertrophy in athletes and nonathletes in the past few decades. Theracurmin, a nutraceutical supplement based on curcumin, has been highlighted by its anti-inflammatory and antioxidant properties in physiological and pathological conditions. This study aimed to investigate the effects of theracurmin intake (300 mg/kg), containing 30 mg/kg of curcumin, in male Swiss mice (n = 66) under distinct protocols of climbing stairs (strength exercise) and their respective detraining period.

Expression of a periplasmic β-glucosidase from Yarrowia lipolytica allows efficient cellobiose-xylose co-fermentation by industrial xylose-fermenting Saccharomyces cerevisiae strains.

This study aimed to compare the effects of cellobiose hydrolysis, whether occurring inside or outside the cell, on the ability of Saccharomyces cerevisiae strains to ferment this sugar and then apply the most effective strategy to industrial S. cerevisiae strains. Firstly, two recombinant laboratory S.

The use of the FRAX® tool and its adjustments in women living with diabetes: a cohort study in primary care in Brazil.

Unlabelled: The FRAX® algorithm showed good accuracy in women living with DM followed in primary care. There were no differences between the ROC curve with and without adjustments for major and hip fractures. The FRAX® 10-year and FRAX® AR were better calibrated in this population.

Production of benzyl-based esters from used soybean cooking oil as renewable plasticizers for flexible PVC films: Exploring new applications for lipases in emerging technologies.

The objective of this study was to produce new and renewable bio-based plasticizers from used soybean cooking oil (USCO). First, USCO was completely converted into free fatty acids (FFAs) using lipase from Candida rugosa. Next, these FFAs were enzymatically esterified with benzyl alcohol in solvent-free systems.

Co-occurrence of arsenic and sewage pollutants in surface and groundwater and its implications for water treatment using membrane technology.

Arsenic (As) enrichment in groundwater stems from natural and hydrogeochemical factors, leading to geological contamination. Groundwater and surface water are interconnected, allowing As migration and surface water contamination. The As contamination poses health risks through contaminated water consumption.

Religion, Religiosity, and Smoking Among Older Adults: Results from the Brazilian Longitudinal Study of Aging (ELSI-Brazil), 2019-2021.

Religion and religiosity have long been associated with various health outcomes and behaviors. This study explored the relationship between religion/religiosity and smoking among 8,703 participants enrolled in The Brazilian Longitudinal Study of Aging (ELSI-Brazil). The logistic regression model was used to test this association, which was then adjusted for sociodemographic factors, health indicators, and alcohol consumption.