One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination.

In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-HRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with the HRP2 antibody (Ab-HRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-HRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy.