A sensitive electrochemical DNA biosensor for detecting the genome of a plant growth-promoting bacteria.

The challenge of increasing food production while maintaining environmental sustainability can be addressed by using biofertilizers such as Azospirillum, which can enhance plant growth and colonize more than 100 plant species. The success of this biotechnology depends on the amount of plant growth-promoting bacteria associated with the plant during crop development. However, monitoring bacterial population dynamics after inoculation requires time-consuming, laborious, and costly procedures.

Vaccination: a look at the social representations of Brazilian children.

Introduction: The pandemic caused by COVID-19 has accentuated the debate on the need for vaccination and called into question the need to increasingly bring this topic, which is widely disseminated in the scientific world, to school classes at all schooling phases. In this scenario, science education plays a key role in disseminating knowledge about the importance of vaccination and the impacting factors of a lack of immunization. In order to better understand this movement, it is necessary to understand the representations of individuals as a way of broadening paths to change this scenario.

Biochemical and histological effects of the subchronic treatment with a beer containing Baccharis dracunculifolia in an experimental model of diabetes.

This article reports the development of a beer made with Baccharis dracunculifolia and its application in an experimental model of diabetes. Initially, the production of the beverage was standardized in order to incorporate the plant extract properly. Next, the beer was analyzed by the UHPLC-MS to identify the substances present.

Hydrolyzed collagen as a partial fat substitute in chicken burgers produced with white striping meat.

White striping (WS) is an anomaly characterized by the appearance of white striations in the Pectoralis major muscles. This study aimed to evaluate the effect of animal fat replacement (chicken skin) with hydrolyzed collagen (HC, 0, 50 and 75%) on the characteristics of burgers with normal chicken breast fillets (FN) and WS. The samples were subjected to physical-chemical characterization, oxidative stability (TBARS), texture, and sensory profile.

Mass Spectrometry-Based Molecular Networks Applied to Study the Molecular Interaction Between Two Endophytic Fungi Isolated From Tibouchina granulosa (Vell.) Cogn (Melastomataceae).

Establishing a microorganism as an endophyte involves complex molecular interactions with its host plant and a broader microbial community. Precise detection methods and comprehensive metabolite annotation are essential to study these interactions. This study focused on characterizing the chemical composition of metabolites produced by two endophytic fungi, Colletotrichum siamense and Xylaria berteroi, isolated from Tibouchina granulosa leaves in axenic conditions and coculture.

Physical Activity and Sedentary Behavior on Bone Health in Adults: A Systematic Review.

The objective was to analyze the longitudinal associations of physical activity (PA) and Sedentary behavior (SB) on bone health indicators (BHI) in healthy young adults. Articles were selected from five databases and 17 longitudinal studies were selected after meeting the inclusion and exclusion criteria. Among these, only one study assessed the association between childhood PA and adult BHI, the results showed no significant association between PA and BHI.

Evaluation of Cluster Algorithms for Radar-Based Object Recognition in Autonomous and Assisted Driving.

Perception systems for assisted driving and autonomy enable the identification and classification of objects through a concentration of sensors installed in vehicles, including Radio Detection and Ranging (RADAR), camera, Light Detection and Ranging (LIDAR), ultrasound, and HD maps. These sensors ensure a reliable and robust navigation system. Radar, in particular, operates with electromagnetic waves and remains effective under a variety of weather conditions.

Modulation of starch-based film properties for encapsulation of microbial inoculant.

Controlled release of beneficial microorganisms in agriculture by encapsulation in biopolymeric matrices can improve biofertilizer efficacy, but it requires the modulation of properties to ensure more efficient and predictable release patterns. This study investigated the effect of a starch-based system to protect and release Priestia megaterium (former Bacillus megaterium) processed as films modified with potential cell-protective additives (maltodextrin, cellulose, and bentonite). The release kinetics, physicochemical and morphological film characteristics, and their protection against UV (Ultraviolet) radiation and temperature were evaluated.

Cascade Cyclization/Annulation of β-Enamino Diketones and -Phenylenediamine: A Strategy to Access Pyrrole-fused 1,5-Benzodiazepines.

Herein, we introduce an unprecedented cascade reaction for the assembly of pyrrole-fused 1,5-benzodiazepine frameworks. These diverse privileged scaffolds were controllably constructed by intramolecular cyclization of β-enamino diketone, followed by annulation with -phenylenediamine. The protocol features efficient one-pot cascade cyclization/annulation, performed under simple and mild reaction conditions.

Development and Characterization of Film-Forming Solution Loaded with (L.) Skeels for Topical Application in Post-Surgical Therapies.

Background/objectives: Considering the antioxidant and antimicrobial properties attributed to compounds in extract, this research aimed to advance postoperative therapeutic innovations. Specifically, the study assessed the physicochemical properties of a film-forming solution (FFS) incorporated with , evaluating its therapeutic potential for postoperative applications.

Methods: The extract was meticulously characterized to determine its chemical composition, with particular emphasis on the concentration of phenolic compounds.

Synthesis of a Bioactive Nitric Oxide-Releasing Polymer from S-Nitrosated Starch.

The incorporation of nitric oxide (NO) into polymeric matrices minimizes degradation and facilitates controlled release. This optimization increases the field of application of NO, in dressings, food protective films, and implant devices, among others. This work presents an economical and easy way to manufacture bioactive nitric oxide-releasing polymer (BioNOR-P) and evaluates its bactericidal and antioxidant activity (AA), mechanical behavior, cytotoxicity, and genotoxicity, seeking future use in different applications.

Sentiment analysis in medication adherence: using ruled-based and artificial intelligence-driven algorithms to understand patient medication experiences.

Background: Studies are exploring ways to improve medication adherence, with sentiment analysis (SA) being an underutilized innovation in pharmacy. This technique uses artificial intelligence (AI) and natural language processing to assess text for underlying feelings and emotions.

Aim: This study aimed to evaluate the use of two SA models, Valence Aware Dictionary for Sentiment Reasoning (VADER) and Emotion English DistilRoBERTa-base (DistilRoBERTa), for the identification of patients' sentiments and emotions towards their pharmacotherapy.

Inhibition of sulfur assimilation by S-benzyl-L-cysteine: Impacts on growth, photosynthesis, and leaf proteome of maize plants.

Sulfur is an essential nutrient for various physiological processes, including protein synthesis and enzyme activation. We aimed to evaluate how S-benzyl-L-cysteine (SBC), an inhibitor of the sulfur assimilation pathway, affects maize plants' growth, photosynthesis, and leaf proteomic profile. Thus, maize plants were grown for 14 days in vermiculite supplemented with SBC.

Reliability of urban microclimate simulations: spatio-temporal validation through intra-urban canyon transects for outdoor thermal comfort analysis.

Mitigating Urban Heat Island (UHI) intensity in cities through adaptative strategies has become an urgent need, as UHI are also exacerbated by climate change impacts imputable to anthropogenic actions. This study addresses the need for reliable simulation models to analyze outdoor thermal comfort (OTC) in future or alternative scenarios. The aim of the present study is to contribute to the validation of CFD urban microclimate simulations by employing intra-urban canyon transects as an alternative or a complementary approach to fixed stations.

Laboratory and physiological aspects of substitute metazoan models for in vivo pharmacotoxicological analysis.

New methods are essential to characterize the performance of substitute procedures for detecting therapeutic action(s) of a chemical or key signal of toxicological events. Herein, it was discussed the applications and advantages of using arthropods, worms, and fishes in pharmacological and/or toxicology assessments. First of all, the illusion of similarity covers many differences between humans and mice, remarkably about liver injury and metabolism of xenobiotics.

Strategies to Increase the Phosphorus Content in the Soil Profile of Vineyards Grown in Subtropical Climates.

Phosphate fertilizers are applied to the soil surface, especially in vineyards in production in subtropical regions. Nowadays, phosphorus (P) is not incorporated into the soil to avoid mechanical damage to the root system in orchards. However, over the years, successive surface P applications can increase the P content only in the topsoil, maintaining low P levels in the subsurface, which can reduce its use by grapevines.

Selective Extraction Process and Characterization of Antioxidant Phenolic Compounds from Leaves Using UPLC-ESI-Q-TOF-MS/MS.

This study innovates in comparing biological activities and chemical composition obtained from extracts and fractions from leaves. Seven extracts and five fractions were produced by conventional successive solid-liquid extraction coupled with simultaneous bioguided purification using solvents of distinct polarities. A comparative analysis was conducted between these purified fractions and the original extracts to elucidate potential improvements in the bioactivity.

Physical fitness components are bone mineral density predictors in adulthood: cross-sectional study.

Background: Health-related physical fitness (HRPF) attributes are considered important markers beneficial to various health outcomes. However, the literature is divergent regarding HRPF and bone health in adulthood, especially due to the end of the second and beginning of the third decades of life when the peak bone mass period occurs.

Objective: To analyze which HRPF variables are areal bone mineral density (aBMD) predictors in adult males and females.

Fractional utilization of the 10-minute treadmill test velocity in running performance.

This study aimed to evaluate the applicability of the 10-minute submaximal treadmill test (T10 test), a self-paced test, in determining critical speed (CS) and predicting running performance. Specifically, we sought to identify the percentage of T10 velocity (vT10) that runners performed in official distance races, and to compare physiological and performance indicators between sexes. 60 recreational runners (n=34 males and n=26 females) underwent a maximum incremental test, the novel T10 test, and ran 1200-m and 2400-m on the track.

Neurodevelopmental changes in Drosophila melanogaster are restored by treatment with lutein-loaded nanoparticles: Positive modulation of neurochemical and behavioral parameters.

Neurodevelopmental disorders, such as autism spectrum disorder (ASD) and attention deficit hyperactivity disorder (ADHD), are characterized by persistent changes in communication and social interaction, as well as restricted and stereotyped patterns of behavior. The complex etiology of these disorders possibly combines the effects of multiple genes and environmental factors. Hence, exposure to insecticides such as imidacloprid (IMI) has been used to replicate the changes observed in these disorders.