[EXPERIENCE IN ORGANIZING THE WORK OF THE CENTER FOR PERSONALIZED TREATMENT OF MALIGNANT NEOPLASMS (NETHERLANDS)].

Introduction: In the last decade, there has been a «breakthrough» development of scientific approaches to the assay of genomic and immune factors underlying anti-cancer treatment efficiency. The choice of antitumor therapy in practice is increasingly determined by molecular signatures, and not only by the specificity of organ tumor originated from (or by the histological properties of the tumor). An urgent and important scientific and practical task is to study the successful experience of organizing Centers for Personalized Cancer Treatment (CPCT).

The Molecular and Cellular Strategies of Glioblastoma and Non-Small-Cell Lung Cancer Cells Conferring Radioresistance.

Ionizing radiation (IR) has been shown to play a crucial role in the treatment of glioblastoma (GBM; grade IV) and non-small-cell lung cancer (NSCLC). Nevertheless, recent studies have indicated that radiotherapy can offer only palliation owing to the radioresistance of GBM and NSCLC. Therefore, delineating the major radioresistance mechanisms may provide novel therapeutic approaches to sensitize these diseases to IR and improve patient outcomes.

The impact of zirconium-89 solution formulation on the efficiency of [ Zr]Zr-deferoxamine synthesis.

In recent years, clinical imaging with Zr-based radiopharmaceuticals has been gaining significant importance in nuclear medicine. This article provides the results of a comparative study of methods for obtaining Zr solutions using ZR, Chelex-100, and TBP resins in the form of [ Zr]Zr-oxalate, [ Zr]Zr-chloride, and [ Zr]Zr-citrate in terms of purification and labeling efficiency. All evaluated methods allowed us to obtain Zr with high yield (>90% in 1 ml).

Evaluation of the Discrimination Ability of the Decision Rule Allowing to Predict the Probability of Death in Oncosurgical Patients with Comorbid Pathology.

At the moment, there are many decision rules and mathematical models that reduce the risk of postoperative mortality and complications. A small part of such medical mathematical models (scales) is successfully used in practice, but there is also a part that eventually remains on the shelves and becomes morally obsolete. The purpose of this work is to evaluate the discrimination ability of the prognostic model underlying the decision rule that allows ranking patients into groups with favorable and unfavorable outcomes and into a group of patients subject to preoperative preparation to maintain the performance of the mathematical model Oncoprognosis 1.

Pt(IV) Prodrugs with Non-Steroidal Anti-inflammatory Drugs in the Axial Position.

We report herein the design, synthesis, and biological investigation of a series of novel Pt(IV) prodrugs with non-steroidal anti-inflammatory drugs naproxen, diclofenac, and flurbiprofen, as well as these with stearic acid in the axial position. Six Pt(IV) prodrugs were designed, which showed superior antiproliferative activity compared to cisplatin as well as an ability to overcome tumor cell line resistance to cisplatin. By tuning the drug lipophilicity via variation of the axial ligands, the most potent Pt(IV) prodrug was obtained, with an enhanced cellular accumulation of up to 153-fold that of cisplatin and nanomolar cytotoxicity both in 2D and 3D cell cultures.

[Indications for lymph node dissection in hepatocellular carcinoma].

Objective: To determine the indications for lymph node dissection in patients with hepatocellular carcinoma.

Material And Methods: The study included 105 patients with hepatocellular carcinoma who underwent dissection of 8, 9, 12, 13 and 16a2 groups of lymph nodes. Concomitant liver diseases were not a contraindication for lymph node dissection.

Hepatoprotective effect of the radiation countermeasure flagellin in the long term after irradiation of mice.

Purpose: Purpose is to study the hepatoprotective effect of a new promising radiation countermeasure flagellin, in the long term after irradiation. The results of the study can be useful for mitigating the consequences of man-made radiation accidents, protecting professional contingents, reducing the toxic effect of radiation therapy, and expanding the range of drug use.

Materials And Methods: Effect of flagellin was investigated 10 months after its administration of irradiated male of mice F1 (CBAхC57Bl/6).

Reliability of Questionnaire-Based Dose Reconstruction: Human Factor Uncertainties in the Radiation Dosimetry of Chernobyl Cleanup Workers.

This original study aims to quantify the human factor uncertainties in radiation doses for Chernobyl cleanup workers that are associated with errors in direct or proxy personal interviews due to poor memory recall a long time after exposure. Two types of doses due to external irradiation during cleanup mission were calculated independently. First, a "reference" dose, that was calculated using the historical description of cleanup activities reported by 47 cleanup workers shortly after the completion of the cleanup mission.

CD44+ and CD133+ Non-Small Cell Lung Cancer Cells Exhibit DNA Damage Response Pathways and Dormant Polyploid Giant Cancer Cell Enrichment Relating to Their p53 Status.

Cancer stem cells (CSCs) play a critical role in the initiation, progression and therapy relapse of many cancers including non-small cell lung cancer (NSCLC). Here, we aimed to address the question of whether the FACS-sorted CSC-like (CD44 + &CD133 +) vs. non-CSC (CD44-/CD133- isogenic subpopulations of p53wt A549 and p53null H1299 cells differ in terms of DNA-damage signaling and the appearance of "dormant" features, including polyploidy, which are early markers (predictors) of their sensitivity to genotoxic stress.

[Algorithms for orthodontic treatment of patients with maxillary constriction based on the stages of formation of the palatal suture].

The Aim Of The Study: Improving the effectiveness of complex orthodontic treatment of patients with constriction and deformation of the upper jaw during the period of replacement and permanent bite based on the analysis of cone-beam computed tomography (CBCT) data.

Materials And Methods: The results of complex treatment of 70 patients with constriction and deformation of the upper jaw during the period of temporary and permanent bite are presented. The patients were divided into groups according to the stage of formation of the midpalatal suture: 1st group - 40 patients with stage «A» and «B» (average age 11.

Reduced Immunosenescence of Peripheral Blood T Cells in Parkinson's Disease with CMV Infection Background.

Immunosenescence is a process of remodeling the immune system under the influence of chronic inflammation during aging. Parkinson's disease (PD) is a common age-associated neurodegenerative disorder and is frequently accompanied by neuroinflammation. On the other hand, cytomegalovirus (CMV), one of the most spread infections in humans, may induce chronic inflammation which contributes to immunosenescence, differentiation and the inflation of T cells and NK cells.

Separation of Sc from Ti in the Context of A Generator System for Radiopharmaceutical Purposes with the Example of [Sc]Sc-PSMA-617 and [Sc]Sc-PSMA-I&T Synthesis.

Today, Sc is an attractive radionuclide for molecular imaging with PET. In this work, we evaluated a Ti/Sc radionuclide generator based on TEVA resin as a source of Sc. The generator prototype (5 MBq) exhibits high Ti retention and stable yield of Sc (91 ± 6 %) in 1 mL of eluate (20 bed volumes, eluent-0.

Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation-Induced Immune Response in Rats.

The development of new laser-driven electron linear accelerators, providing unique ultrashort pulsed electron beams (UPEBs) with low repetition rates, opens new opportunities for radiotherapy and new fronts for radiobiological research in general. Considering the growing interest in the application of UPEBs in radiation biology and medicine, the aim of this study was to reveal the changes in immune system in response to low-energy laser-driven UPEB whole-body irradiation in rodents. Forty male albino rats were exposed to laser-driven UPEB irradiation, after which different immunological parameters were studied on the 1st, 3rd, 7th, 14th, and 28th day after irradiation.

Cytogenetic analysis (buccal micronucleus cytome assay) of radioactive waste management workers.

The Russian Federation is implementing a program for dismantling nuclear-powered submarines and nuclear service vessels decommissioned from the Navy. Program personnel may be exposed to mutagenic and carcinogenic factors, including ionizing radiation. Improved preclinical health diagnostics are desirable, for timely identification of health effects, optimization of preventive measures, and medical interventions.

[Prospects for using low-dose radiation in the complex therapy for COVID-19].

This review presents the literature data of new approaches for the treatment of COVID-19 with low doses of radiation (LDR). In addition, data on the use of LDR for the treatment of various disorders, in particular pneumonia, a number of inflammatory processes of various etiology, as well as Alzheimer's disease are discussed. The mechanisms of LDR action are briefly described, associated with the activation of the immune system and antiinflammatory response due to the effect on the processes of oxidative stress, which is reflected in an increase in the activity of cytokines (interleukin- (IL-) 6), changes in the expression of a number of genes (such as P53 and NF-κB (p65)) and long non-coding RNAs (ncRNAs) (the authors' own data are presented).

Radiation survey and environmental impact assessment at the site of temporary storage at Andreeva Bay (16 years of studies).

The site of temporary storage for spent fuel and radioactive waste at Andreeva Bay is a largest nuclear legacy site in the Russian Northwest. The radiation situation within the industrial site and in the surrounding health protection zone (HPZ) is characterised by significant local contamination of the topsoil, which is a source of environmental contamination and potential spread of radioactivity within and outside the HPZ, including the adjacent marine environment. The highest levels of radioactive contamination of soil due to manmade radionuclides have been registered around the spent nuclear fuel storage facilities, where the specific activity of Cs-137 reaches 5 × 10Bq kg, and that of Sr-90, 5.

Comprehensive environmental monitoring during remediation of a nuclear legacy site in the Far East of Russia.

In compliance with the fundamentals of the government's policy on nuclear and radiation safety, Russia is continuing to implement its national program for decommissioning its nuclear legacy. Under this program, the State Atomic Energy Corporation, 'Rosatom', is carrying out decommissioning and remediation of the Navy's former shore technical bases (STBs) in the Russian Far East. Along with the 'old problems' accumulated during the operation of STBs in the past, new challenges arise because of the planned intensification of radioactive waste (RW) management on the site in the nearest future.

Effects of combined exposure to modeled radiation and gravitation factors of the interplanetary flight: Monkeys' cognitive functions and the content of monoamines and their metabolites; cytogenetic changes in peripheral blood lymphocytes.

In a study on primates (Macaca mulatta), neurobiological and radiobiological effects have been studied of the synchronous combined action of 7-day antiorthostatic hypokinesia and exposure of the monkeys' head first to γ-rays during 24 h and then to accelerated C ions. The neurobiological effects were evaluated by the cognitive functions which model the basic elements of operator activity and the concentration of monoamines and their metabolites in peripheral blood. The radiobiological effects were evaluated by the chromosomal aberration and DNA double-strand break (DSB) yield in peripheral blood lymphocytes.

Immunocytochemical Localization of XRCC1 and γH2AX Foci Induced by Tightly Focused Femtosecond Laser Radiation in Cultured Human Cells.

To assess the prospects for using intense femtosecond laser radiation in biomedicine, it is necessary to understand the mechanisms of its action on biological macromolecules, especially on the informational macromolecule-DNA. The aim of this work was to study the immunocytochemical localization of DNA repair protein foci (XRCC1 and γH2AX) induced by tightly focused femtosecond laser radiation in human cancer A549 cells. The results showed that no XRCC1 or γH2AX foci tracks were observed 30 min after cell irradiation with femtosecond pulses of 10 W∙cm peak power density.

Impact of the Spectral Composition of Kilovoltage X-rays on High-Z Nanoparticle-Assisted Dose Enhancement.

Nanoparticles (NPs) with a high atomic number () are promising radiosensitizers for cancer therapy. However, the dependence of their efficacy on irradiation conditions is still unclear. In the present work, 11 different metal and metal oxide NPs (from Cu ( = 29) to BiO ( = 83)) were studied in terms of their ability to enhance the absorbed dose in combination with 237 X-ray spectra generated at a 30-300 kVp voltage using various filtration systems and anode materials.

Russian Consensus on Exo- and Endocrine Pancreatic Insufficiency After Surgical Treatment.

The Russian consensus on exo- and endocrine pancreatic insufficiency after surgical treatment was prepared on the initiative of the Russian Pancreatic Club using the Delphi method. Its goal was to consolidate the opinions of national experts on the most relevant issues of diagnosis and treatment of exo- and endocrine insufficiency after surgical interventions on the pancreas. An interdisciplinary approach is ensured by the participation of leading gastroenterologists and surgeons.