Knee phenotypes distribution according to CPAK classification in Turkish population.

Objective: This study aimed to investigate the distribution of knee phenotypes based on the CPAK classification in healthy nonarthritic subjects and osteoarthritic patients in Türkiye.

Methods: Radiological EOS analysis of nonarthritic 1172 knees and osteoarthritic 571 knees was evaluated to clarify the distribution of CPAK classification. The knees were categorized into 9 subgroups according to the arithmetic hip-knee-ankle (aHKA) angle and joint-line obliquity (JLO).

In-vitro susceptibility of pathogenic and intermediate Leptospira species towards antibiotics and herb extracts.

Leptospirosis is a severe and potentially fatal re-emerging zoonotic and waterborne disease caused by pathogenic and intermediate species of Leptospira. Given the high global rates of morbidity and mortality associated with this disease, there is an urgent need to explore alternative therapeutic agents to enhance treatment options. This study investigates the anti-leptospiral efficacy of several common antibiotics-penicillin G, doxycycline, ampicillin, amoxicillin, cefotaxime, chloramphenicol, and erythromycin, as well as extracts from local herbs, Hydnophytum formicarum Jack and Boesenbergia stenophylla, against pathogenic and intermediate Leptospira strains.

Metarhizium anisopliae: current status and future in hard ticks control in Asia.

Ticks exert a significant economic impact on the livestock industry, particularly in Asian regions. Presently, chemical acaricides constitute the primary method employed to combat tick infestations in livestock, but their use carries adverse environmental consequences. Overreliance on acaricides has contaminated milk and meat products with chemical residues while fostering tick resistance to these agents due to improper and intensive application.

Blood meal analysis: unveiling the feeding preferences of Aedes aegypti and Aedes albopictus in a dengue-endemic area.

Studies have suggested animals as possible reservoir hosts for flaviviruses transmitted by Aedes mosquitoes; however, there is limited evidence for the dengue virus in Malaysia. One of the possible ways to determine the zoonotic potential for any pathogen transmission is through blood meal analysis which can provide valuable insights into the feeding preferences of the mosquitoes. Unfortunately, limited information is available on the feeding preferences of Aedes mosquitoes in Malaysia.

Potential induction of apoptosis and acetylcholinesterase inhibition in Aedes mosquitoes by Streptomyces-derived ethyl acetate extract.

The use of Streptomyces secondary metabolites for mosquito control has recently received positive attention. Accordingly, this study was performed to elucidate the cellular, genomic and biochemical responses of Aedes mosquitoes to Streptomyces sp. KSF103 ethyl acetate (EA) extract, a mixture previously characterized for its potential bioactivity.

Association of total and dengue-specific IgE levels in the sera with dengue virus inhibition and antibody-dependent enhancement.

Dengue, caused by the dengue virus (DENV), poses a significant global health challenge. Effective vaccines and treatments for dengue are lacking due to gaps in understanding its pathogenesis and mechanisms in severe cases. This study investigates the role of immunoglobulin E (IgE) in dengue, focusing on its potential association with virus neutralization and antibody-dependent enhancement (ADE) in DENV replication.

Natural intestinal parasite infection in synanthropic migratory Barn Swallow, Hirundo rustica, in central Peninsular Malaysia.

Migratory birds are potential vectors transmitting zoonotic parasites, bacteria, and viruses that can significantly affect human health. Over 60 diseases have been identified to be associated with these birds. Given their close proximity to human habitation, the potential transmission of zoonotic diseases is a major concern for the public.

Anthelmintic activity and pathophysiological effect of Allium sativum crude extract against carcinogenic liver fluke, Opisthorchis viverrini.

Opisthorchis viverrini poses a substantial risk for cholangiocarcinoma (CCA) in Thailand. Despite praziquantel (PZQ) remains the primary treatment for opisthorchiasis, its association with adverse effects and potential CCA development during prolonged treatment, particularly in cases of reinfection and chronic infection, underscores the imperative for alternative herbal interventions with anthelmintic potential. In this context, a prior study suggested the inhibitory effects of Allium sativum L.

Case reports of Strongyloides stercoralis infection in three patients with haematological malignancies.

Strongyloidiasis is a parasitic nematode infection mainly caused by Strongyloides stercoralis. Immunocompromised conditions, particularly cancer patients treated with chemotherapy and corticosteroids, have a significant risk of developing Strongyloides hyperinfection. The lack of a gold standard laboratory method to rule out this infection and the insensitivity of microscopic stool examination due to low and intermittent larvae output in stool contribute to the low detection rate of this infection.

First molecular detection of hemotropic Mycoplasma spp. and molecular screening of other vector-borne pathogens in camels from the greater Cairo metropolitan area, Egypt.

In Egypt, knowledge about vector-borne bacterial pathogens in camels remains limited. To address this gap, 181 blood samples from adult one-humped camels (Camelus dromedarius) in the greater Cairo metropolitan area were collected from October 2021 to March 2022. Through PCR assays, four pathogens were detected, where Anaplasmataceae being the most common (54.

First report of Anaplasma marginale and Anaplasma ovis in goats in Kelantan, Malaysia.

Anaplasma species are obligate rickettsial intraerythrocytic pathogens that cause an important tick-borne disease of economic importance in livestock production in many countries. Anaplasma species have been detected from farm animals worldwide, there is a paucity of information on Anaplasma infections in goats from Malaysia. Thus, this study aimed to assess the infection rate and identify Anaplasma species and some selected risk factors in goats across selected districts in Kelantan, Malaysia.

Detection of Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in shelter and pet dogs in Malaysia.

Canine haemotrophic mycoplasmosis is caused by mycoplasma haemopathogens, which includes Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp). The Mhc and CMhp pose a health risk to dogs, particularly in immunocompromised and splenectomised dogs, as they lead to haemolytic anaemia. There is scarce information on the detection of Mycoplasma in dogs in Malaysia.

Molecular screening of Babesia, Hepatozoon, and Theileria (Apicomplexa: Piroplasmida) in ticks (Acari: Ixodidae) infesting farm ruminants in Peninsular Malaysia.

Ticks are obligate hematophagous arachnids that feed on both humans and animals. Despite the extensive research on detection of bacteria in ixodid ticks in Malaysia, there remains limited knowledge about the detection of protozoa in these ectoparasites, especially in those that feed on farm ruminants. In this study, 1,241 ticks belonging to four species (Rhipicephalus microplus, R.

Molecular identification and phylogenetic analysis of Anaplasma marginale and Anaplasma centrale isolated from commercial Mafriwal cattle in Johor, Malaysia.

Bovine anaplasmosis is a tick-borne disease in cattle which is mainly caused by Anaplasma marginale and Anaplasma centrale. It poses significant economic burdens and threat on livestock industries worldwide. This study aimed to identify Anaplasma spp.

Simultaneous total hip and knee arthroplasties in hemophilic arthropathy: series of 5 cases.

Objective: This study aimed to compare preoperative and postoperative measures in haemophiliacs who had simultaneous total hip and knee arthroplasties.

Methods: A retrospective database search identified five patients with severe factor 8 deficiencies who underwent simultaneous hip and knee joint replacement surgery between 2002-2018. Preoperative and postoperative evaluations included Harris Hip Score (HHS), Knee Society Score (KSS), Knee Injury and Osteoarthritis Outcome Score (KOOS), range of motion, flexion contracture (FC), Visual Analog Scale (VAS), hip-knee angle, and leg length discrepancy.

Friction of Laser-Textured Zirconia Coated with a Platelet-Rich Fibrin.

In the present in vitro study, we evaluated the adhesion of an injectable platelet-rich fibrin (i-PRF) to laser-textured zirconia surfaces and their resultant friction behavior against bone tissue. Three types of zirconia surfaces were compared regarding the i-PRF coating effects: 1) grit blasted with 250-μm spherical alumina particles and acid etched with 20% hydrofluoric acid (ZLA), 2) laser textured with a random (RD) surface pattern, or 3) laser textured with a designed pattern based on 16 lines and 8 passages (L16N8). The coefficient of friction (COF) of the specimens was assessed on a reciprocating sliding pin-on-plate tribometer at 1-N normal load, 1 Hz, and a 2-mm stroke length.

Molecular and functional profiling unravels targetable vulnerabilities in colorectal cancer.

Colorectal cancer (CRC) patients with microsatellite-stable (MSS) tumors are mostly treated with chemotherapy. Clinical benefits of targeted therapies depend on mutational states and tumor location. Many tumors carry mutations in KRAS proto-oncogene, GTPase (KRAS) or B-Raf proto-oncogene, serine/threonine kinase (BRAF), rendering them more resistant to therapies.

Body mass index is similar to alternative anthropometric indices in evaluating plasma lipids as proxy for cardiovascular disease in women with previous hypertensive disorders of pregnancy: A cross-sectional study.

Background: Women with previous hypertensive disorders of pregnancy (HDP) have increased risk of cardiovascular disease (CVD). Overweight is a modifiable risk factor for both conditions. Anthropometric indices such as waist circumference, hip circumference, waist-to-hip ratio, estimated total body fat, a body shape index, waist-to-hip-to-height ratio, and index of central obesity improve estimation of cardiovascular death risk in the general population as compared to body mass index (BMI).

Epoetin alfa has a potent anxiolytic effect on naive female rats.

Background: Epoetin alfa is a derivative of the erythropoietin hormone. This study aims to investigate the epoetin alfa effect on anxiety-like behaviors.

Methods: Adult female Wistar Albino rats were divided into Control (n = 8), 1000 U Epoetien alfa, and 2000 U Epoetien alpha.

Cytisine compared to combination nicotine replacement therapy to reduce cigarette consumption in relapsed smokers: protocol for a pilot randomized controlled trial.

Background: Cigarette smoking is a leading cause of death and disease, including those related to the cardiovascular system. Cytisine is a plant-based medication, which works in a similar mechanism to varenicline. It is safe, efficacious, and cost-effective for smoking cessation.

Ester derivatives of Dictyostelium differentiation-inducing factors exhibit antibacterial activity, possibly via a prodrug-like function.

Objective: Dictyostelium differentiation-inducing factors 1 and 3 [DIF-1 (1) and DIF-3 (2), respectively], along with their derivatives, such as Ph-DIF-1 (3) and Bu-DIF-3 (4), demonstrate antibacterial activity in vitro against Gram-positive bacteria, including methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant S. aureus (MRSA), vancomycin-sensitive Enterococcus faecalis (VSE), and vancomycin-resistant Enterococcus faecium [VRE (VanA)]. This study investigates the therapeutic potential of DIF compounds against these Gram-positive bacteria.

Long COVID and its risk factors in migrants: a nationwide register study from Sweden.

Background: Many studies have found more severe COVID-19 outcomes in migrants and ethnic minorities throughout the COVID-19 pandemic, while recent evidence also suggests higher risk of longer-term consequences. We studied the risk of a long COVID diagnosis among adult residents in Sweden, dependent on country of birth and accounting for known risk factors for long COVID.

Methods: We used linked Swedish administrative registers between March 1, 2020 and April 1, 2023, to estimate the risk of a long COVID diagnosis in the adult population that had a confirmed COVID-19 infection.

Assessing safety culture and second victim experience following adverse events among Romanian nurses: a cross-sectional study.

Background: Healthcare adverse events (AEs) significantly impact professionals, often leading to emotional distress and lasting effects. This study investigates the impact of AEs on healthcare professionals in Romania, focusing on nurses to examine their experiences within the patient safety culture and the psychological consequences of AEs. With a limited body of research on patient safety, adverse events, and second victims (SVs) in Romania, this study addresses a crucial gap, highlighting the need for enhanced safety culture and support mechanisms for SVs.

Steatohepatitis-induced vascular niche alterations promote melanoma metastasis.

Background: In malignant melanoma, liver metastases significantly reduce survival, even despite highly effective new therapies. Given the increase in metabolic liver diseases such as metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic dysfunction-associated steatohepatitis (MASH), this study investigated the impact of liver sinusoidal endothelial cell (LSEC)-specific alterations in MASLD/MASH on hepatic melanoma metastasis.

Methods: Mice were fed a choline-deficient L-amino acid-defined (CDAA) diet for ten weeks to induce MASH-associated liver fibrosis, or a CDAA diet or a high fat diet (HFD) for shorter periods of time to induce early steatosis-associated alterations.

To what extent are digital health interventions targeting HIV care cascade among mobile populations feasible, acceptable, and effective? A mixed methods systematic review protocol.

Introduction: Human mobility is associated with an increased risk of HIV acquisition and disengagement from HIV care, leading to poorer health outcomes among highly mobile individuals compared to less mobile individuals. Mobile individuals, broadly defined as those who temporally, seasonally, or permanently move from one place to another for voluntary or involuntary reasons, face many challenges in accessing HIV care services. These challenges include logistical difficulties, interruptions in HIV care continuity, and limited access to services across different locations, which together hinder timely testing, treatment initiation, and viral suppression.