Multiplexing biochemical signals.

In this Letter we show that living cells can multiplex biochemical signals, i.e., transmit multiple signals through the same signaling pathway simultaneously, and yet respond to them very specifically.

Local investigation of the optical properties of subwavelength rectangular holes with a focused beam of electrons.

The optical properties of rectangular subwavelength holes in a gold film are investigated using the light generated when a focused beam of electrons impinges on the sample close to the hole. Using this technique, multi-spectral maps of the holes are obtained with a resolution beyond the optical diffraction limit. The results show the influence of hole shape on the spectrum of locally scattered light.

Force generation by dynamic microtubules in vitro.

Biopolymers are essential for cellular organization. They bridge the cell interior, forming a framework that is used as a reference for different cellular organelles. This framework, called the cytoskeleton, is not static but constantly reorganizes.

Wavelength-selective addressing of visible and near-infrared plasmon resonances for SU8 nanolithography.

We imprint plasmonic near field enhancements as nanoscale topography in SU8 photoresist using two-photon absorption from a spectrally filtered broadband supercontinuum light source. Imprinted patterns smaller than 50 nm across are obtained localized at positions of high local field enhancements in gold bow tie antennas, and gold split rings resonant in the visible and near-infrared. Enhanced exposure only occurs at wavelengths and polarizations that exactly match the plasmonic resonances.

Ultrafast reciprocal space investigation of cavity-waveguide coupling.

Local information on the coupling mechanism between the photonic crystal nanocavity and the feeding waveguide is crucial to enable further improvements of the performance of these systems. Although several investigations on such a coupling have already been performed, information on the local dynamic properties remains hidden. Here, we present a reciprocal space investigation of the dynamics of light side-coupled to a photonic crystal nanocavity.

Quantitative coherent anti-Stokes Raman scattering (CARS) microscopy.

The ability to observe samples qualitatively at the microscopic scale has greatly enhanced our understanding of the physical and biological world throughout the 400 year history of microscopic imaging, but there are relatively few techniques that can truly claim the ability to quantify the local concentration and composition of a sample. We review coherent anti-Stokes Raman scattering (CARS) as a quantitative, chemically specific, and label-free microscopy. We discuss the complicating influence of the nonresonant response on the CARS signal and the various experimental and mathematical approaches that can be adopted to extract quantitative information from CARS.

A semi-classical model of attosecond electron localization in dissociative ionization of hydrogen.

In the development of attosecond molecular science, a series of experiments have recently been performed where ionic fragment asymmetries in the dissociative ionization of H(2) into H(+) + H and that of D(2) into D(+) + D were used to uncover electron localization processes that occur on the attosecond and few-femtosecond timescale. Electron localization was observed both in strong-field dissociative ionization using carrier envelope phase-stable few-cycle laser pulses [Kling et al., Science, 2006, 312, 246] and in a two-color extreme ultra-violet + infrared attosecond pump-probe experiment [Sansone et al.

Experimental observation of evanescent modes at the interface to slow-light photonic crystal waveguides.

We experimentally study the fields close to an interface between two photonic crystal waveguides that have different dispersion properties. After the transition from a waveguide in which the group velocity of light is v(g) ~ c/10 to a waveguide in which it is v(g) ~ c/100, we observe a gradual increase in the field intensity and the lateral spreading of the mode. We attribute this evolution to the existence of a weakly evanescent mode that exponentially decays away from the interface.

Control of light transmission through opaque scattering media in space and time.

We report the first experimental demonstration of combined spatial and temporal control of light transmission through opaque media. This control is achieved by solely manipulating spatial degrees of freedom of the incident wave front. As an application, we demonstrate that the present approach is capable of forming bandwidth-limited ultrashort pulses from the otherwise randomly transmitted light with a controllable interaction time of the pulses with the medium.

Uptake of gold nanoparticles in healthy and tumor cells visualized by nonlinear optical microscopy.

Understanding the mechanism underlying the interactions between inorganic nanostructures and biological systems is crucial for several rapidly growing fields that rely on nano-bio interactions. In particular, the further development of cell-targeted drug delivery using metallic nanoparticles (NP) requires new tools for understanding the mechanisms triggered by the contact of NPs with membranes in different cells at the subcellular level. Here we present a novel concept of multimodal microscopy, enabling three-dimensional imaging of the distribution of gold NPs in living, unlabeled cells.

Direct observation of size fractionation during colloidal crystallization.

We present a confocal microscopy study of the quasi-two-dimensional crystallization of a binary mixture of spherical colloids coated with long DNA strands. Our experiments show that in the crystalline phase the two colloidal species are completely demixed. Analysis of the lattice spacings in the two types of colloidal crystal shows that the diameters of the two species of colloids differ by 10%.

Three-dimensional negative index of refraction at optical frequencies by coupling plasmonic waveguides.

We identify a route towards achieving a negative index of refraction at optical frequencies based on coupling between plasmonic waveguides that support backwards waves. We show how modal symmetry can be exploited in metal-dielectric waveguide pairs to achieve negative refraction of both phase and energy. Control of waveguide coupling yields a metamaterial consisting of a one-dimensional multilayer stack that exhibits an isotropic index of -1 at a free-space wavelength of 400 nm.

On the use of Purcell factors for plasmon antennas.

The Purcell factor is the standard figure of merit for spontaneous emission enhancement in microcavities and has also been proposed to describe emission enhancements for plasmonic resonances. A comparison of quality factor, mode volume, and Purcell factor for single and coupled plasmon spheres to exact calculations of emission rates shows that a Purcell factor derived from quality factor and mode volume does not describe emission changes due to plasmon antennas.

A new imaging method for understanding chemical dynamics: efficient slice imaging using an in-vacuum pixel detector.

The implementation of the Timepix complementary metal oxide semiconductor pixel detector in velocity map slice imaging is presented. This new detector approach eliminates the need for gating the imaging detector. In time-of-flight mode, the detector returns the impact position and the time-of-flight of charged particles with 12.

Fast, high resolution mass spectrometry imaging using a Medipix pixelated detector.

In mass spectrometry imaging, spatial resolution is pushed to its limits with the use of ion microscope mass spectrometric imaging systems. An ion microscope magnifies and then projects the original spatial distribution of ions from a sample surface onto a position-sensitive detector, while retaining time-of-flight mass separation capabilities. Here, a new type of position-sensitive detector based on a chevron microchannel plate stack in combination with a 512 × 512 complementary metal-oxide-semiconductor based pixel detector is coupled to an ion microscope.

Magnetic light-matter interactions in a photonic crystal nanocavity.

We study the magnetic coupling between a metal-coated near-field probe and a photonic crystal nanocavity. The resonance of the nanocavity shifts to shorter wavelengths when the ringlike apex of the probe is above an antinode of the magnetic field of the cavity. We show that this can be attributed to a magnetic light-matter interaction and is in fact a manifestation of Lenz's law at optical frequencies.

Spatial threshold in amplifying random media.

We study experimentally as well as numerically the transport and generation of light in multiple scattering media with optical gain. By imaging the spatial distribution of light escaping from the side of the sample, the propagation depth is analyzed. Far below and far above random laser threshold, the spatial profile of emission light is independent of pump intensity, while around threshold, the spatial distribution of emission light changes profoundly.

Characterization of bending losses for curved plasmonic nanowire waveguides.

We characterize bending losses of curved plasmonic nanowire waveguides for radii of curvature ranging from 1 to 12 microm and widths down to 40 nm. We use near-field measurements to separate bending losses from propagation losses. The attenuation due to bending loss is found to be as low as 0.

Negative-index metamaterials: looking into the unit cell.

With their potential for spectacular applications, like superlensing and cloaking, metamaterials are a powerful class of nanostructured materials. All these applications rely on the metamaterials acting as a homogeneous material. We investigate a negative index metamaterial with a phase-sensitive near-field microscope and measure the optical phase as a function of distance.

Label-free imaging of lipophilic bioactive molecules during lipid digestion by multiplex coherent anti-Stokes Raman scattering microspectroscopy.

The digestion and absorption of lipophilic, bioactive molecules such as lipids, physiologically active nutrients (nutraceuticals), and drugs play a crucial role in human development and health. These molecules are often delivered in lipid droplets. Currently, the kinetics of digestion of these lipid droplets is followed by in vitro models that simulate gastrointestinal conditions, while phase changes within the lipid droplets are observed by light or electron microscopy.

Cooperativity in ion hydration.

Despite prolonged scientific efforts to unravel the effects of ions on the structure and dynamics of water, many open questions remain, in particular concerning the spatial extent of this effect (i.e., the number of water molecules affected) and the origin of ion-specific effects.

Molecular restructuring of water and lipids upon the interaction of DNA with lipid monolayers.

Understanding the molecular mechanism of DNA/lipid interaction is critical in optimizing the use of lipid cofactors in gene therapy. Here, we address this question by employing label-free vibrational sum frequency (VSF) spectroscopy to study the interaction of DNA with lipid monolayers of the cationic lipids DPTAP(1,2-dipalmitoyl-3-trimethylammonium-propane) and diC14-amidine as well as the zwitterionic lipid DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) in the presence and absence of calcium. Our approach has the advantage both of allowing us to explicitly probe intermolecular interactions and of providing insight into the structure of water and lipids around DNA at the lipid interface.

Electric and magnetic dipole coupling in near-infrared split-ring metamaterial arrays.

We present experimental observations of strong electric and magnetic interactions between split ring resonators (SRRs) in metamaterials. We fabricated near-infrared planar metamaterials with different inter-SRR spacings along different directions. Our transmission measurements show blueshifts and redshifts of the magnetic resonance, depending on SRR orientation relative to the lattice.