[Cataracts extraction combined with multifocal intraocular lens optic implantation in Berger space in school-age children with cataracts].

To evaluate the safety and efficacy of cataract extraction combined with multifocal intraocular lens (IOL) optic implantation in Berger space in school-age children with cataracts. It was a prospective study. The clinical data of school-age children with cataract who underwent cataract extraction combined with multifocal IOL implantation at Qingdao Eye Hospital of Shandong First Medical University from January 2019 to June 2023 were collected.

Daily activities change is linked to acute angle closure occurrence in COVID-19 co-infected patients.

Objectives: To analyze the influence of daily activity-related factors associated with COVID-19 infection on the occurrence of acute angle closure (AAC).

Methods: A multicenter hospital-based study was conducted at 23 ophthalmic centers in 17 provincial-level regions across China to recruit patients with confirmed AAC during the post-lockdown time of COVID-19 (P-TOC) from Dec 7, 2022, to Jan 17, 2023, and three lockdown time of COVID-19 (TOC) periods, which included the TOC-2022 (Sep 7, 2022 - Dec 6, 2022), TOC-2021(Sep 7, 2021 - Jan 6, 2022) and TOC-2020 (Sep 7, 2020 - Jan 6, 2021). Patient information, including demographic, a questionnaire on daily activity changes during the AAC period, COVID-19 history, and eye examination results, was collected.

Role of mA methyltransferase METTL3 in keratoconus pathogenesis.

Keratoconus (KC) is the most common ectatic corneal disease with unknown pathogenesis. This study aimed to investigate the role of methyltransferase-like enzyme 3 (METTL3) in KC pathogenesis. In the present study, we examined the levels of METTL3 and other N6-methyladenosine (mA) modification-related proteins in KC samples and human stromal keratocyte (HTK) cells stimulated by mechanical stretch (MS) using Western blotting and immunohistochemistry.

Clinical Characteristics and Prognostic Factors of Patients with Intraocular Foreign Bodies from a Tertiary Eye Center in North China.

Background: This study aims to describe the epidemiology and clinical features of patients with intraocular foreign bodies (IOFBs) and analyze the prognostic factors influencing final vision.

Methods: We retrospectively reviewed medical records of patients with IOFBs admitted to Qingdao Eye Hospital of Shandong First Medical University between January 1, 2014, and December 31, 2021. Inclusion criteria involved complete clinical data details of patients diagnosed with intraocular foreign bodies in our hospital who were treated with concurrent surgery and based on IOFB position and characteristics, different surgical methods were employed.

3D printing of stiff, tough, and ROS-scavenging nanocomposite hydrogel scaffold for in situ corneal repair.

Despite significant advancements in hydrogels in recent years, their application in corneal repair remains limited by several challenges, including unfitted curvatures, inferior mechanical properties, and insufficient reactive oxygen species (ROS)-scavenging activities. To address these issues, this study introduces a 3D-printed corneal scaffold with nanocomposite hydrogel consisting of gelatin methacrylate (GelMA), poly (ethylene glycol) diacrylate (PEGDA), Laponite, and dopamine. GelMA and PEGDA act as matrix materials with photo-crosslinking abilities.

Update on central factors in myopia development beyond intraocular mechanisms.

Myopia, a prevalent refractive error, primarily affects children and adolescents, characterized by excessive axial elongation causing distant objects to focus in front of the retina. This review explores the intricate mechanisms beyond intraocular factors, emphasizing the significant role of central factors in myopia development and progression. Intraocular mechanisms involving the retina, RPE/choroid, and sclera are well documented, with these structures playing crucial roles in eye growth regulation.

Analysis of the spatio-temporal evolutionary characteristics of myopia among students aged 7-18 years in China: based on panel data analysis.

Myopia is a major important public health concern and has huge burden on health system across the world. This study aimed to explore the spatial and temporal analysis of the prevalence of myopia among students aged 7-18 years to identify the potential clusters and the trends of myopia in China. Spatial autocorrelation analysis, temporal and spatial scan analysis and beta convergence test were performed using ArcGIS10.

Targeting reactive oxygen species to ameliorate T cell-mediated inflammation in dry eye syndrome: a novel therapeutic approach.

Dry eye syndrome (DES) is a prevalent condition linked to oxidative stress from Orthokeratology (OK) lens use, causing significant discomfort and impacting quality of life. Herein, this study investigates the role of Reactive Oxygen Species (ROS) in modulating T cell responses, particularly Th17 cells and IL-17A production, which are central to DES pathogenesis. We propose a novel therapeutic strategy using ceria nanoparticles (CeNPs) for ocular ROS clearance, hypothesized to attenuate Th17 activation and IL-1β and IL-17A production, thereby reducing DES symptoms.

Automated measurement and correlation analysis of fundus tessellation and optic disc characteristics in myopia.

This study aims to quantify fundus tessellated (FT) density and optic disc (OD) morphology using deep learning (DL) techniques and to investigate the correlations between these fundus characteristics and refractive function in young patients with myopia. We constructed two DL-based segmentation models to delineate the FT, OD, peripapillary atrophy (PPA), and macula at a pixel-level resolution. The study sought to identify differences in fundus characteristics between eyes categorized as having high myopia versus mild or moderate myopia.

The ROS/AKT/S6K axis induces corneal epithelial dysfunctions under LED blue light exposure.

In recent years, concerns have escalated regarding eye health problems arising from Light-emitting diode (LED), which emits high-energy blue light (BL), potentially causing corneal epithelial dysfunctions (CEpD). Nevertheless, the mechanisms underlying this damage remain poorly comprehended. This study endeavors to explore the specific mechanisms through which BL exposure induces CEpD.

Comprehensive landscape of RNA N6-methyladenosine modification in lens epithelial cells from normal and diabetic cataract.

To gain more insight into the mechanism of cataract formation from the perspective of epigenetics in the diabetic population, lens epithelium from diabetic cataract patients and health individuals were collected separately and analyzed for N6-methyladenosine (mA)-modified RNA using methylated RNA immunoprecipitation sequencing (MeRIP-Seq). Subsequently, differential expression analysis was performed on mA-regulated messenger RNA (mRNA), circular RNA (circRNA), and long non-coding RNA (lncRNA), followed by functional annotation using the Gene Ontology (GO) database. Furthermore, analysis of single-cell data of lens complemented the intrinsic association and cellular heterogeneity of cataract and mA regulators.

Long-term Nerve Regeneration in Diabetic Keratopathy Mediated by a Novel NGF Delivery System.

Diabetic keratopathy (DK) is a common chronic metabolic disorder that causes ocular surface complications. Among various therapeutic approaches, local delivery of nerve growth factor (NGF) remains the most effective treatment of DK. However, achieving a sustained therapeutic effect with NGF and the frequent drug delivery burden remain challenging during clinical practice.

Visual rehabilitation using rigid gas permeable contact lenses after femtosecond laser-assisted minimally invasive lamellar keratoplasty in patients with keratoconus.

This study aims to observe the clinical efficacy and safety of rigid gas permeable corneal contact lenses (RGP-CLs) wearing after femtosecond laser-assisted minimally invasive lamellar keratoplasty (FL-MILK) in progressive and advanced keratoconus eyes. Twenty-five patients (27 eyes) fitted with RGP-CLs after FL-MILK were enrolled, and 22 grading-matched keratoconus patients (23 eyes) as a control group. Corneal morphological data, diopter, best corrected vision, corneal endothelium, non-invasive tear film rupture time (NIBUT), corneal perception and comfort questionnaire were analysed before and after wearing RGP-CLs.

[A single-center study on the distribution and shifting trend of infectious keratitis pathogens from 2018 to 2022].

To investigate the pathogen species, composition, and distribution characteristics of infectious keratitis pathogens in Shandong Province and its surrounding areas. In this cross-sectional study, patients with keratitis who underwent corneal sampling and microbiological culture at the Shandong Eye Hospital from January 1, 2018 to December 31, 2022 were included. Under topical anesthesia, the edge of the lesion was scraped by an experienced physician.

Preventing and treating neurotrophic keratopathy by a single intrastromal injection of AAV-mediated gene therapy.

Purpose: Neurotrophic keratopathy (NK) is a degenerative corneal condition resulting from corneal nerve injury. Current therapies, including the recombinant human nerve growth factor (rhNGF) therapy, requires continuous administration. This study aims to develop a novel and highly effective gene therapy strategy for the prevention and treatment of NK.

Recent advances in NLRP3 inflammasome in corneal diseases: Preclinical insights and therapeutic implications.

NLRP3 inflammasome is a cytosolic multiprotein complex formed in response to exogenous environmental stress and cellular damage. The three major components of the NLRP3 inflammasome are the innate immunoreceptor protein NLRP3, the adapter protein apoptosis-associated speck-like protein containing a C-terminal caspase activation and recruitment domain, and the inflammatory protease enzyme caspase-1. The integrated NLRP3 inflammasome triggers the activation of caspase-1, leading to GSDMD-dependent pyroptosis and facilitating the maturation and release of inflammatory cytokines, namely interleukin (IL)-18 and IL-1β.

Using first-eye early vault to determine implantable collamer lens size for the second eye: retrospective analysis of bilateral phakic intraocular lens implantation.

Purpose: To evaluate the role of first-eye vault after the implantation of implantable collamer lens (ICL) V4c in selecting ICL size for the second eye and analyze the changing characteristics of the vault.

Setting: Qingdao Eye Hospital of Shandong First Medical University, Qingdao, China.

Design: Retrospective case series.

RTP4 Enhances Corneal HSV-1 Infection in Mice With Type 2 Diabetes Mellitus.

Purpose: The purpose of this study was to investigate whether corneal lesions in mice with type 2 diabetes mellitus (T2D) infected with herpes simplex virus (HSV)-1 are more severe, and to elucidate the specific underlying mechanism.

Methods: The corneas of control mice and T2D mice induced by a high-fat diet combined with streptozotocin were infected with the HSV-1 Mckrae strain to assess corneal infection, opacity, and HSV-1 replication. RNA sequencing of the corneal epithelium from wild-type and db/db mice (a genetic T2D mouse model) was conducted to identify the key gene affecting T2D infection.