Untargeted metabolomics and PacBio analysis on bioactive components and microbial community in co-fermentation of black soldier fly larva.

Fermentation can enhance nutritional value and safety of insect protein, this study utilized probiotic Bacillus subtilis (B. subtilis) and complex enzyme containing chitinase and protease to ferment the paste of Black Soldier Fly larva (BSFL), decomposing anti-nutritional factor chitin and protein in paste while inhibiting the proliferation of harmful microorganisms. The result indicated a 40 % degradation of chitin after fermentation, accompanied by an increase in the variety and quantity of amino acids and peptides, functional substances such as raffinose and cucurbitacin significantly increased, while the levels of antibiotics such as erythromycin and ofloxacin had decreased; after fermentation, there is a significant difference in the microbial distribution between bacteria, co-fermentation and CK, the indigenous microbiota of BSF and pathogenic bacteria such as Klebsiella pneumoniae and Clostridiaceae bacteria were significantly inhibited, anaerobic bacteria, including Anaerosalibacter, Caldicoprobacter and Tissierella, exhibit a marked increase; significant changes are detected in the carbon sources, amino acids, and key enzymes related to other metabolic pathways of B.

Butyl benzyl phthalate induces neurotoxicity in Eisenia fetida: Mechanisms revealed by biochemical and metabolomic analyses.

Phthalates, particularly butyl benzyl phthalate (BBP), are ubiquitous environmental contaminants with potential neurotoxic effects. However, their impact on soil organisms, especially earthworms (Eisenia fetida), remains poorly understood. The current study investigated the neurotoxic effects of BBP on Eisenia fetida in artificial and red soils using an integrated approach combining biochemical assays, metabolomics, and molecular docking.

Hot spots of resistance: Transit centers as breeding grounds for airborne ARG-carrying bacteriophages.

The presence of pathogenic bacteria and antibiotic resistance genes (ARGs) in urban air poses a significant threat to public health. While prevailing research predominantly focuses on the airborne transmission of ARGs by bacteria, the potential influence of other vectors, such as bacteriophages, is often overlooked. This study aims to investigate the characteristics of phages and ARGs in aerosols originating from hospitals, public transit centers, wastewater treatment plants, and landfill sites.

Spatiotemporal response of the optical characteristics of dissolved organic matter to seasonality and land use in tropical island rivers.

Dissolved organic matter (DOM), a pivotal component in the global carbon cycle, plays a crucial role in maintaining the productivity and functionality of aquatic ecosystems. However, the driving factors of variations in the properties of riverine DOM in tropical islands still remain unclear. In this study, the spatiotemporal response of the optical characteristics of riverine DOM to seasonality and land use on Hainan Island in southern China was investigated.

Differences in the uptake and translocation of differentially charged microplastics by the taproot and lateral root of mangroves.

The interception of microplastics (MPs) by mangrove roots plays an indispensable role in reducing the environmental risks of MPs. However, there remains limited research on the fate of the intercepted MPs. Hereby, the uptake and subsequent translocation of 0.

Soil organic matter priming: The pH effects.

Priming of soil organic matter (SOM) decomposition by microorganisms is a key phenomenon of global carbon (C) cycling. Soil pH is a main factor defining priming effects (PEs) because it (i) controls microbial community composition and activities, including enzyme activities, (ii) defines SOM stabilization and destabilization mechanisms, and (iii) regulates intensities of many biogeochemical processes. In this critical review, we focus on prerequisites and mechanisms of PE depending on pH and assess the global change consequences for PE.

Microbial resistance and resilience to drought and rewetting modulate soil NO emissions with different fertilizers.

Future climate models indicate an enhanced severity of regional drought and frequent rewetting events, which may cause cascading impacts on soil nitrogen cycle and nitrous oxide (NO) emissions, but the underlying microbial mechanism remains largely unknown. Here we report an incubation study that examined the impacts of soil moisture status and nitrification inhibitor (DCD) on the NO-producers and NO-reducers following the application of urea and composted swine manure in an acid soil. The soil moisture treatments included 100 % water-holding capacity (WHC) (wetting, 35.

Residual effects of chlorinated organic pollutants on microbial community and natural redox processes in coastal wetlands.

Chlorinated organic pollutants (COPs) are common in flooded environments. To examine the residual status and effects of COPs on flooded environments, a survey of 7 coastal wetlands in Zhejiang, East China was conducted. Total COP concentrations detected from 95.

Environmental damages, cumulative exergy demand, and economic assessment of Panus giganteus farming with the application of solar technology.

The use of photovoltaic (PV) technology in agricultural production can mitigate the environmental impacts of mushroom farming. However, changes in the environmental impacts and economic benefits of the application of PV technology are still unclear. Thus, we evaluated the environmental impacts, energy flow, and economic aspects of mushroom (Panus giganteus) farming systems without solar PV (WS) technology and with PV technology from the generation of substrate materials through harvesting.

The role of pores in micro-zone distribution of Cd in a tropical paddy soil: Results from X-ray computed tomography combined energy dispersive spectroscopy analysis.

Accurate description of Cd micro-zone distribution and accumulation is the prerequisite for revealing Cd transfer and transformation processes. However, to date, the role of soil pores in the Cd micro-zone distribution characteristics in undisturbed soil is still unclear. In this study, the obvious heterogeneous distribution of Cd in and around the soil pores at the cross-sectional surface of the tropical undisturbed topsoil was visualized by the combination of X-ray micro-computed tomography and scanning electron microscope-energy dispersive spectroscopy.

An unrecognized entry pathway of submicrometre plastics into crop root: The split of hole in protective layer.

Threats to food safety caused by the continuous accumulation of plastic particles in the terrestrial environment is currently a worldwide concern. To date, descriptions of how plastic particles pass the external biological barrier of crop root have been vague. Here, we demonstrated that submicrometre polystyrene particles passed unimpededly the external biological barrier of maize through the split of holes in the protective layer.

Implications of vermicompost on antibiotic resistance in tropical agricultural soils - A study in Hainan Island, China.

The contamination of antibiotic resistance genes (ARGs) associated with animal manure fertilization have attracted a global concern. Vermicompost has been widely popularized as an eco-friendly alternative to recycle animal manure on Hainan Island, China. However, the effects of vermicompost application on ARG spread and environmental fate in tropical agricultural soils remains undefined.

Role of mangrove forest in interception of microplastics (MPs): Challenges, progress, and prospects.

Mangroves receive microplastics (MPs) from terrestrial, marine and atmospheric sources, acting as a huge filter for environmental MPs between land and sea. Due to the high primary production and complex hydrodynamic conditions in mangroves, MPs are extensively intercepted in various ways while flowing through mangroves, leading to a long-standing but fiercely increasing MPs accumulation. However, current researches mainly focused on the occurrence, source and fate of MPs pollution in mangroves, ignoring the role of mangrove forests in the interception of MPs.

Comparing the variations and influencing factors of CH emissions from paddies and wetlands under CO enrichment: A data synthesis in the last three decades.

Understanding and quantifying the impact of elevated tropospheric carbon dioxide concentration (e [CO]) on methane (CH) globally is important for effectively assessing and mitigating climate warming. Paddies and wetlands are the two important sources of CH emissions. Yet, a quantitative synthetic investigation of the effects of e [CO] on CH emissions from paddies and wetlands on a global scale has not been conducted.

Distinct distribution patterns and functional potentials of rare and abundant microorganisms between plastisphere and soils.

The increasing application of plastic film has caused the "white pollution" of farmlands in greenhouses. To date, most studies on the ecology of the plastisphere have focused on the whole microbial community, with few on the rare and abundant taxa, especially in the terrestrial ecosystems. To understand the plastisphere rare and abundant taxa of bacterial and fungal communities, we collected residues of plastic film from plastic-covered soils in the greenhouse.

Fertilizer-induced NO and NO emissions in tea gardens and the main controlling factors: A recent three-decade data synthesis.

Tea gardens have been widely documented to be hotspots for nitrogen (N) oxide emissions (i.e., nitrous oxide (NO) and nitric oxide (NO)).

Transfer and distribution of antibiotic resistance genes in the soil-peanut system receiving manure for years.

Antibiotic resistance gene (ARG)-contaminated food from manure application is gaining widespread interest, but little is known about the distribution and uptake of ARGs in peanuts that are subjected to manure routinely. In this study, the ARG profile and bacterial community in soil and peanut plants from a 7-year manure-fertilized field were investigated using high-throughput qPCR and 16S rRNA gene sequencing. Manure application increased the abundance of ARGs in soil and peanuts by 59-72 and 4-10 fold, respectively.

Metagenomic binning analyses of pig manure composting reveal potential antibiotic-degrading bacteria and their risk of antibiotic resistance genes.

Antibiotic-degrading bacteria are commonly used to treat antibiotic contamination, but the antibiotic resistance genes (ARGs) they carry are often overlooked. This study used metagenomic assembly and binning analyses to explore potential antibiotic-degrading bacteria and their ARGs during pig manure composting. The result showed that 35 metagenome-assembled genomes (MAGs) mainly containing alkyl-aryl transferase and decarboxylase genes involved in the removal of antibiotics.

Phosphorus fractions and iron oxides responsible for the variations in the Cd retention capacity in tropical soil aggregates under different cropping patterns.

The environmental behavior of Cd in soil has been widely studied because of its close relationship with food security and soil environmental pollution. In this study, the roles of P fractions and Fe oxides in the retention of Cd in typical tropical soil from five cropping patterns were investigated. Although there was no evident relationship between the Cd adsorption capacity and soil aggregate particle sizes, strong spatial associations of P, Fe, and Cd at the soil aggregates were observed via energy dispersive spectroscopy analysis.

Application of in Practical Biotechnology of Industrial Recovery of Potato Starch By-Products and Its Flocculation Characteristics.

This study developed a practical recovery for potato starch by-products by and applied it on a plant scale to completely solve the pollution problems. Soughing to evaluate the effect of applied towards the production of by-products recycling and analyze the composition and characteristics of flocculating substances (FS) by and advance a possible flocculation mechanism for by-product conversion. After fermentation, the chemical oxygen demand (COD) removal rate, and the conversion rates of cellulose, hemicellulose, pectin, and proteins were 58.

Priming of soil organic carbon mineralization and its temperature sensitivity in response to vegetation restoration in a karst area of Southwest China.

Plant residue input alters native soil organic carbon (SOC) mineralization through the priming effect, which strongly controls C sequestration during vegetation restoration. However, the effects of different vegetation types on SOC priming and the underlying microbial mechanisms due to global warming are poorly understood. To elucidate these unknowns, the current study quantified soil priming effects using C-labeled maize residue amendments and analyzed the community structure and abundances in the soils of a vegetation succession gradient (maize field (MF), grassland (GL), and secondary forest (SF)) from a karst region under two incubation temperatures (15 °C and 25 °C).

Antagonistic effect of polystyrene nanoplastics on cadmium toxicity to maize (Zea mays L.).

Nanoplastics (NPs) (<1 μm) have gradually attracted worldwide attention owing to their widespread occurrence, distribution, and ecosystem risks. Few studies have explored the interaction between NPs and heavy metals in crops. In this study, we investigated the influence of polystyrene nanoplastics (PSNPs; 10 mg/L and 100 mg/L) and cadmium (2 mg/L and 10 mg/L) on the physiological and biochemical indices of maize plants, grown in Hoagland solution with contaminants, for 14 days.

Co-composting with cow dung and subsequent vermicomposting improve compost quality of spent mushroom.

In order to determine a feasible degrading process for spent mushroom (SMS) with high lignin content, the present work used cow dung (CD), SMS, and a mixture of CD and SMS as substrates and evaluated the effects of vermicomposting on the microflora and the quality of composting products. Bacterial (R = 0.548, P = 0.

A 14-amino acids deletion in BmShadow results to non-moult on the 2nd instar in the bivoltine silkworm, Bombyx mori.

The Bombyx mori Shadow gene (BmShadow) belongs to the superfamily of cytochrome P450 genes. To elucidate the function of the BmShadow gene and its association with diapause, we employed the CRISPR/Cas9 system to knock out the BmShadow gene in the bivoltine strain Qiufeng. The mutant (BmShadow) was obtained in G, exhibiting a 42-base deletion corresponded exactly to the amino acids regions from positions 155 to 168.

Sorption of norfloxacin, sulfamerazine and oxytetracycline by KOH-modified biochar under single and ternary systems.

Pollution of water by single antibiotics has been investigated in depth. However, in reality, a wide range of different contaminants is often mixed in the aquatic environment (contaminant cocktail). Here, single and competitive sorption dynamics of ionizable norfloxacin (NOR), sulfamerazine (SMR) and oxytetracycline (OTC) by both pristine and modified biochars were investigated.