Bioactive potency of extracts from and with silver nanoparticles against cancer cell lines and pathogenic bacteria.

Silver nanoparticles (AgNPs) are spherical particles with a number of specific and unique physical (such as surface plasmon resonance, high electrical conductivity and thermal stability) as well as chemical (including antimicrobial activity, catalytic efficiency and the ability to form conjugates with biomolecules) properties. These properties allow AgNPs to exhibit desired interactions with the biological system and make them prospective candidates for use in antibacterial and anticancer activities. AgNPs have a quenching capacity, which produces reactive oxygen species and disrupts cellular processes (such as reducing the function of the mitochondria, damaging the cell membrane, inhibiting DNA replication and altering protein synthesis).

Phenotypic identification of Metallo-ß- lactamase resistance Gram negative bacteria from a clinical specimen in Sidama, Ethiopia.

Background: Metallo-beta lactamase resistance is one of the carbapenem resistances that worsen the world nowadays. A new variant of carbapenem-resistant has only limited reports from Africa including Ethiopia. This study aimed to determine Metallo -ß- lactamase resistance Gram-negative bacteria in Hawassa University Comprehensive Specialized Hospital January-June 2023.

Characterization of Metallo β-Lactamase Producing Isolates with Susceptibility to the Aztreonam/Avibactam Combination.

Metallo-β-lactamases (MBLs) in and other Gram-negative organisms pose significant public health threats due to their association with multidrug resistance (MDR). Although aztreonam (AZT) can target MBL-producing organisms, its efficacy is compromised in organisms expressing additional β-lactamases that inactivate it. Combining AZT with the β-lactamase inhibitor avibactam (AVI) may restore its activity against MBL-producing isolates.

National Cohort of Compassionate Use of Meropenem-Vaborbactam: No Benefit over Meropenem for .

Background: Meropenem-vaborbactam (MEM-VAB) is a novel carbapenem-beta-lactamase-inhibitor combination that demonstrates activity against carbapenem-resistant (CR) Gram-negative bacteria, and more specifically KPC-producers, since vaborbactam is an effective inhibitor of KPC enzymes in vitro. This study aimed to describe the initial uses and efficacy of MEM-VAB for compassionate treatment during the first 21 months following its early access in France.

Method: A national multicenter retrospective study was conducted, including all patients who received at least one dose of MEM-VAB between 20 July 2020, and 5 April 2022.

Enhanced Effect of Patient Room Disinfection Against Carbapenem-Resistant and Methicillin-Resistant Using UV-C Irradiation in Conjunction with UV-C Containment Unit.

: In environments with high-frequency contact surfaces, drug-resistant bacteria, such as carbapenem-resistant and methicillin-resistant (MRSA), can survive for extended periods, contributing to healthcare-associated infections. Ultraviolet (UV)-C irradiation often fails to adequately disinfect shadowed areas, leading to a persistent contamination risk. We evaluated the effectiveness of using a UV-C containment unit (UVCCU) in conjunction with UV-C irradiation to improve the sterilization effects on both direct and indirect surfaces, including shadowed areas, and to assess the leakage of UV radiation to the surroundings.

Presence of multidrug-resistant bacteria on ready-to-use laryngoscope blades and handles: a cross-sectional study.

Background: The presence of microorganisms in laryngoscopes emphasizes the risk to patient safety during orotracheal intubations.

Methods: Cross-sectional study was carried out in university hospital in the inpatient, emergency, intensive care and surgical center sectors. Microorganisms were recovered from the blades using a filter membrane and from the handles using swab.

Prevalence and Antibiotic Susceptibility of Pathogenic Enterobacteria Strains from Three Biotopes in the City of Ouagadougou (Burkina Faso).

Purpose: The emergence of antibiotic resistance in pathogenic is a public health problem in tropical countries such as Burkina Faso. Antibiotic resistance could be identified using a variety of approaches. This study aimed to estimate the prevalence of pathogenic enterobacteria strains from three sources, as well as their antibiotic resistance profile to biotope and climatic season.

Prevalence of difficult-to-treat resistance in ESKAPE pathogens in a third level hospital in Mexico.

Background: Antimicrobial resistance and difficult-to-treat resistance (DTR) in ESKAPE pathogens ( and species) is a threat to human health. The aim of this study was to determine the prevalence of antimicrobial resistance and DTR rates in ESKAPE pathogens over six years in a third-level hospital from Monterrey, Mexico.

Methods: Antimicrobial susceptibility testing was determined by either disk diffusion or broth microdilution in strains from 2018 to 2023.

Anti-biofilm effect of ferulic acid against and : and investigation.

and , key members of the ESKAPE group of hospital-acquired pathogens, are driving forces behind numerous infections due to their potent biofilm formation and the growing threat of antimicrobial resistance. Ferulic acid (FA) is known for its strong antioxidant properties and is recognized for its numerous physiological benefits, including anti-inflammatory, antimicrobial, anticancer, and antidiabetic effects. The current investigation delves into the antimicrobial and antibiofilm ability of FA against and .

Effect of Tanreqing injection on multidrug resistance organisms: A test-negative case-control study and network pharmacology analysis.

Background: Multidrug resistance organisms (MDROs) pose a major threat in intensive care units (ICUs). Although in vitro studies suggested that Tanreqing (TRQ) was effective against MDROs, evidence about TRQ injection usage and its real-world effectiveness is lacking.

Purpose: This study aimed to investigate treatment pattern and real-world effectiveness of TRQ against MDRO infections among ICU patients being treated with antibiotics.

Neglected class A carbapenemases: Systematic review of IMI/NmcA and FRI from a One Health perspective.

Carbapenemase-producing Enterobacterales are pathogens classified as a critical priority by the World Health Organization and a burden on human health worldwide. IMI, NmcA, and FRI are under-detected class A carbapenemases that have been reported in the human, animal and environmental compartments, particularly these last 5 years. Bacteria producing these carbapenemases have been mostly identified in digestive carriage screenings, but they are also involved in severe infections, such as bacteremia.

Short-term clinical outcomes of subway-related amputations.

Introduction: In city hospitals, subway-related traumatic amputations are a frequent pattern of injury, however there is a paucity of literature on this specific injury pattern. The purpose of this study was to describe the epidemiology of subway-related traumatic amputations, as well as compare them to non-subway traumatic amputations.

Patients And Methods: Retrospective review was performed at a single Level-1 trauma center in a metropolitan area.

Apical periodontitis microbiome association with salivary and serum inflammatory burden.

Aims: Apical Periodontitis (AP) involves complex interactions between the root canal microbiome and the host immune response, with potential risk of local and systemic inflammatory burden, however there is no evidence available regarding correlation between microbiome and inflammatory marker levels. This study aims to identify the microbiome of saliva, intracanal and blood samples in AP subjects and investigate the correlation between intracanal and blood microbiomes with serum inflammatory biomarker levels, and salivary microbiomes with salivary inflammatory biomarker levels.

Methodology: Saliva, Intracanal and blood samples were collected from AP patients undergoing root canal retreatment.

First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh.

Enterobacter asburiae (E. asburiae) is a gram-negative rod-shaped bacterium which has emerging significance as an opportunistic pathogen having high virulence pattern and drug resistant properties. In this study, we present the detailed analysis of the whole genome sequence of a multidrug-resistant (MDR) E.

Clinical and microbiological analyses of colistin-resistant strains among carbapenem-resistant complex clinical isolates.

Carbapenem-resistant complex (CR-ECC), which is rapidly increasing as the cause of nosocomial infections, has limited treatment options. The aim of this study is to investigate the microbiological and clinical traits and molecular epidemiology of isolates of CR-ECC and provide guidance for antibiotic selection in clinical practice. Clinical CR-ECC isolates (ertapenem MIC ≥ 2 mg/L) were collected from 2021 to 2022.

A fucose-binding superlectin from Enterobacter cloacae with high Lewis and ABO blood group antigen specificity.

Bacteria frequently employ carbohydrate-binding proteins, so-called lectins, to colonize and persist in a host. Thus, bacterial lectins are attractive targets for the development of new antiinfectives. To find new potential targets for antiinfectives against pathogenic bacteria, we searched for homologs of Pseudomonas aeruginosa lectins and identified homologs of LecA in Enterobacter species.

Characterization of Enterobacter phage vB_EcRAM-01, a new Pseudotevenvirus against Enterobacter cloacae, isolated in an urban river in Panama.

The Enterobacter cloacae complex, a prominent bacterium responsible worldwide for most bloodstream infections in the hospital environment, has shown broad-spectrum antibiotic resistance, including carbapenems. Therefore, bacteriophages have again attracted the attention of the science and medical community as an alternative to control Multidrug resistant bacteria. In this study, water samples from Río Abajo River, in Panama City, Panama, were collected, for phage isolation, purification, characterization and propagation against the E.

Emergence of an unconventional Enterobacter cloacae-derived Iturin A C-15 as a potential therapeutic agent against methicillin-resistant Staphylococcus aureus.

Antimicrobial resistance poses a significant global health threat by reducing the effectiveness of conventional antibiotics, particularly against pathogens like Methicillin-resistant Staphylococcus aureus (MRSA). This study investigates the antimicrobial potential of rhizospheric soil bacteria from Prosopis cineraria (Sangri) in the Thar Desert. Bacterial strains isolated from these samples were observed to produce secondary metabolites, notably, Iturin A C-15 cyclic lipopeptide (SS1-3-P) which was extracted from strain Enterobacter cloacae SS1-3 and was purified and characterized using reverse-phase HPLC, ESI-LC/MS, Nile-Red Assay, and FT-IR analysis.

Bed occupancy and nosocomial infections in the intensive care unit: A retrospective observational study in a tertiary hospital.

Background: Healthcare-associated infections (HAI) are a major problem globally, contributing to prolonged hospital admissions and poor outcomes.

Objectives: To examine HAI incidence and risk factors in an intensive care unit (ICU) during high v. low occupancy periods.

Intraoperative Predictors of Salvage in Infected Breast Implants: A Retrospective Study.

Background: Infectious complications associated with implant-based breast reconstruction (IBBR) can be devastating and may lead to reconstructive failure. While there are known demographic risk factors for reconstructive failure, few studies have identified intraoperative findings that predict failure after attempted salvage.

Objectives: The objective of this study was to identify intraoperative findings in infected breasts that may be predictive of implant failure.

Prevalence, Distribution and Antimicrobial Susceptibility of Enterobacteriaceae and Non-Fermenting Gram-Negative Bacilli Isolated From Environmental Samples in a Veterinary Clinical Hospital in Madrid, Spain.

Managing infections caused by multidrug-resistant Gram-negative bacilli is a major public health concern, particularly in hospitals where surfaces can act as reservoirs for resistant microorganisms. Identifying these bacteria in hospital environments is crucial for improving healthcare safety. This study aimed to analyse environmental samples from a veterinary hospital to identify prevalent microorganisms and detect antimicrobial resistance patterns.

Multiple regional outbreaks caused by global and local VIM-producing Klebsiella pneumoniae clones in Poland, 2006-2019.

Purpose: This study was aimed at comprehensive genomic analysis of VIM-type carbapenemase-producing Klebsiella pneumoniae species complex (KpSC) in Poland.

Methods: All non-duplicate 214 VIM-producing KpSC isolates reported in Poland in 2006-2019 were short-read sequenced and re-identified by the average nucleotide identity scoring. Their clonality/phylogeny was assessed by cgMLST and SNP in comparison with genomes from international databases.

Molecular characterization of virulence genes and influence of Xanthium strumarium extract against two Enterobacter species isolated from some soil invertebrates.

The development of bacterial antibiotic resistance poses a danger to healthcare systems worldwide. To reduce the spread of disease, researchers are looking for novel measures to control bacterial infections to reduce the spread of disease. The antibacterial properties of Xanthium strumarium methanolic and ethanolic extracts were evaluated against Enterobacter cloacae and E.