Engineering thermostability of industrial enzymes for enhanced application performance.

Thermostability is a key factor for the industrial application of enzymes. This review categorizes enzymes by their applications and discusses the importance of engineering thermostability for practical use. It summarizes fundamental theories and recent advancements in enzyme thermostability modification, including directed evolution, semi-rational design, and rational design.

Magnetic fluorinated mesoporous metal-organic frameworks for rapid derivatization-assisted GC-MS analysis of perfluoroalkyl carboxylic acids in harsh water environment.

A novel magnetic mesoporous fluorinated metal-organic framework material (FeO@MIP-206-F) has been synthesized specifically for application as an adsorbent for perfluoroalkyl carboxylic acids (PFCAs) extraction by magnetic solid-phase extraction (MSPE). The carefully designed FeO@MIP-206-F material features an appropriate porosity, open metal sites of Zr, and functional groups (fluorine and amino) conducive to the adsorption process. The distinctive architecture of the material endows it with exceptional extraction capabilities for PFCAs.

Copper-cobalt diatomic bifunctional oxygen electrocatalysts based on three-dimensional porous nitrogen-doped carbon frameworks for high-performance zinc-air batteries.

Transition-metal-loaded carbon-based electrocatalysts are promising alternatives to conventional precious metal electrocatalysts for the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) in high-performance zinc-air batteries. However, efficiently doping transition-metal single atoms onto carbon-based frameworks is a significant challenge. Herein, an improved template-sacrificing method combining a two-step carbonization process is proposed to fabricate Cu/Co diatomic sites coanchored on a three-dimensional nitrogen-doped carbon-based framework.

An Oral HS Responsive CuO Nanozyme Platform with Strong ROS/HS Scavenging Capacity for the Treatment of Colitis.

Inflammatory bowel disease involves excess reactive oxygen species (ROS) and hydrogen sulfide (HS) at inflammatory sites. Nanozyme-mediated ROS and HS scavenging therapy is promising for colitis treatment. Here, we synthesized a multiple ROS scavenging CuO nanoparticle and first explored its HS scavenging capacity.

COBRA-LIKE 9 modulates cotton cell wall development via regulating cellulose deposition.

Plant cell walls are complex and dynamic cellular structures critical for plant growth, development, physiology, and adaptation. Cellulose is one of the most important components of the cell wall. However, how cellulose microfibrils deposit and assemble into crystalline cellulose remains elusive.

Overproduction of Cucurbitadienol through Modular Metabolic Engineering and Fermentation Optimization in .

Cucurbitadienol is a key intermediate in the biosynthesis of cucurbitane-type compounds and serves as a precursor for mogrosides, cucurbitacins, and other valuable natural products of potential biological and food importance. However, microbial fermentation for cucurbitadienol production remains inefficient, limiting its potential for further industrial application. This study achieved the efficient synthesis of cucurbitadienol through a multimodular strategy.

Diffusion model assisted designing self-assembling collagen mimetic peptides as biocompatible materials.

Collagen self-assembly supports its mechanical function, but controlling collagen mimetic peptides (CMPs) to self-assemble into higher-order oligomers with numerous functions remains challenging due to the vast potential amino acid sequence space. Herein, we developed a diffusion model to learn features from different types of human collagens and generate CMPs; obtaining 66% of synthetic CMPs could self-assemble into triple helices. Triple-helical and untwisting states were probed by melting temperature (Tm); hence, we developed a model to predict collagen Tm, achieving a state-of-art Pearson's correlation (PC) of 0.

Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology.

Metabolic engineering-driven microbial cell factories have made great progress in the efficient bioproduction of biochemical and recombinant proteins. However, the low efficiency and robustness of microbial cell factories limit their industrial applications. Harnessing microbial heterogeneity contributes to solving this.

Crosslink among cyclin-dependent kinase 9, ATP binding cassette transporter G2 and Beclin 1 in colorectal cancer.

Colorectal cancer (CRC) ranks third in the number of cancers mainly because of the inability to diagnose it at an early stage. The pathogenesis of CRC is complicated, which is the result of the complex interaction of multiple genetic and environmental factors. Currently, one of the main treatments for CRC is chemotherapy.

Engineering for Efficient Synthesis of Salidroside.

Salidroside is a high-value plant-derived glycoside with diverse biological activities, but the main industrial salidroside production method, extraction from plants, is insufficient to meet the growing market demand. The biosynthetic route via microbial fermentation is a sustainable and eco-friendly alternative method. synthesis of the precursor tyrosol was established by introducing the and genes.

Targeting p62 by sulforaphane promotes autolysosomal degradation of SLC7A11, inducing ferroptosis for osteosarcoma treatment.

Osteosarcoma (OS) is the most prevalent malignant bone tumor in children and adolescents worldwide. Identification of novel therapeutic targets and development of targeted drugs are one of the most feasible strategies for OS treatment. Ferroptosis, a recently discovered mode of programmed cell death, has been implicated as a potential strategy for cancer therapy.

Construction of a charge-reversal polyelectrolyte nanocarrier for targeted intestinal releasing of kidney tea saponin based on sodium alginate/ε-polylysine/alliin.

Herein, a pH-responsive delivery system based on sodium alginate (ALG), ε-polylysine (PLL) and alliin (ALL) has been designed. The innovative use of the charged nature of alliin to prepare carriers loaded with kidney tea saponins has rarely been reported in the literature before. The size and morphology of the complex was quantified by dynamic light scattering (DLS) analysis and scanning electron microscopy (SEM), exhibiting a size of 141 ± 1 nm.

Research on cross-provincial power trading strategy considering the medium and long-term trading plan.

To accommodate China's electricity market reforms integrating medium and long-term (MLT) transactions and spot transactions, and to boost renewable energy consumption through the spot market, this paper proposes an optimized cross-provincial electricity trading strategy model based on a two-layer game framework. The proposed model incorporates an MLT green certificate contract decomposition method, enabling nested optimization of green certificate contracts and scheduling plans for cross-provincial power transactions. To encourage broader participation, a bilateral green certificate trading framework is established, which globally optimizes green certificate allocation to increase benefits for market participants.

Engineering enzyme conformation within liquid-solid hybrid microreactors for enhanced continuous-flow biocatalysis.

The artificial engineering of an enzyme's structural conformation and dynamic properties to promote its catalytic activity and stability outside cellular environments is highly pursued in industrial biotechnology. Here, we describe an elegant strategy of combining the rationally designed liquid-solid hybrid microreactor with a tailor-made polyethylene glycol functional ionic liquid (PEG-IL) microenvironment to exercise a high level of control over the configuration of enzymes for practical continuous-flow biocatalysis. As exemplified by a lipase driven kinetic resolution reaction, the obtained system exhibits a 2.

High-level production of patatin in Pichia pastoris and characterization of N-glycosylation modification in food processing properties.

Patatin is an acidic protein found in potatoes that is commonly used in food and pharmaceutical industries due to its excellent emulsifying and gelation abilities. Pichia pastoris is widely used as a host for recombinant protein production because it can incorporate post-translational modifications. In this study, a patatin titre of 2189.

The transcription factor GhMYB4 represses lipid transfer and sucrose transporter genes and inhibits fiber cell elongation in cotton.

Cotton (Gossypium hirsutum) fiber is a highly elongated single cell with a thickened cell wall. MYB transcription factors are important regulators of plant cell elongation; however, the molecular mechanism involved in regulating fiber elongation remains to be explored. Here, we present evidence that the R2R3-MYB transcription factor GhMYB4 negatively regulates cotton fiber cell elongation by suppressing the expression of 2 crucial genes previously reported to affect fiber development: lipid transfer protein 4 (GhLTP4) and sucrose transporter 12 (GhSWEET12).

A Golgi vesicle-membrane-localized cytochrome B561 regulates ascorbic acid regeneration and confers Verticillium wilt resistance in cotton.

Ascorbic acid (AsA) serves as a key antioxidant involved in the various physiological processes and against diverse stresses in plants. Due to the insufficiency of AsA de novo biosynthesis, the AsA regeneration is essential to supplement low AsA synthesis rates. Redox reactions play a crucial role in response to biotic stress in plants; however, how AsA regeneration participates in hydrogen peroxide (HO) homeostasis and plant defense remains largely unknown.

Bryophytes adapt to open-pit coal mine environments by changing their functional traits in response to heavy metal-induced soil environmental changes.

Plants have unique adaptability to heavy metal pollution. However, the adaptation strategies of bryophytes are still unclear. In order to better understand the response of bryophytes to different heavy metal and the adaptation mechanisms of different species to heavy metal pollution, we studied soil physicochemical properties, distribution of heavy metal elements, ecological risk assessment and the community structure, functional characteristics of bryophytes in large open-pit coal mines in Inner Mongolia.

Re-localization of a repeat-containing fungal effector by apoplastic protein Chitinase-like 1 blocks its toxicity.

A fungal effector that is toxic to plant cells was identified in Verticillium dahliae. The effector contains a non-canonical Common in several Fungal Extracellular Membrane proteins (CFEM) domain, a tandem repeat region consisting of four 14-amino acid repeats rich in proline, and a C-terminal region, thus is designated V. dahliae tetrapeptide repeat protein (VdTRP).

A FeCo-Se@NiCo-PO Electrode Designed by Hierarchical Strategy for Supercapacitors and NiCo//Bi Batteries.

In this work, FeCo-Se and NiCo-PO were electrodeposited on nickel foam (NF) successively to prepare a cathode material for asymmetric supercapacitors (ASCs) and NiCo//Bi batteries. FeCo-Se@NiCo-PO combines the advantages of transition metal selenides (TMSs) and transition metal phosphates (TMPs). FeCo-Se electrodeposited in the underlying layer can facilitate electron transfer for higher conductivity.

Design of super stretchability, rapid self-healing, and self-adhesion hydrogel based on starch for wearable strain sensors.

Since hydrogels are conductive, easily engineered, and sufficiently flexible to imitate the mechanical properties of human skin, they are seen as potential options for wearable strain sensors. However, it is still a great challenge to prepare a hydrogel through simple and straightforward methods that integrate excellent stretchability, ionic conductivity, toughness, self-adhesion, and self-healing. Herein, an acrylamide/3-acrylamide phenylboronic acid cross-linked network is modified to produce a semi-interpenetrating cross-linked hydrogel in just one easy step by adding starch.

Chiral Inorganic Polar BaTiO/BaCO Nanohybrids with Spin Selection for Asymmetric Photocatalysis.

Chirality-dependent photocatalysis is an emerging domain that leverages unique chiral light-matter interactions for enabling asymmetric catalysis driven by spin polarization induced by circularly polarized light selection. Herein, we synthesize chiral inorganic polar BaTiO/BaCO nanohybrids for asymmetric photocatalysis via a hydrothermal method employing chiral glucose as a structural inducer. When excited by opposite circularly polarized light, the same material exhibits significant asymmetric catalysis, while enantiomers present an opposite polarization preference.

Rational design of lanosterol 14α-demethylase for ergosterol biosynthesis in .

Unlabelled: Ergosterol is widely used in skin care products and drug preparation. Lanosterol 14α-demethylase (Erg11p, 14DM, CYP51) is the rate-limiting enzyme for the biosynthesis of various steroid compounds in . Herein, Erg11p was engineered to extend the in vivo catalytic half-life and increase the turnover rate.