Unraveling the genetic basis of azoospermia: transcriptome profiling analyses in a Greek population.

Objective: To investigate whether idiopathic nonobstructive azoospermia (iNOA) has its own transcriptomic signature.

Design: Testicular tissue biopsies were retrieved, processed, and prepared for ribonucleic acid (RNA) extraction from 26 consented patients diagnosed with iNOA. Samples were grouped into four pools based on the presence of testicular spermatozoa: two replicate pools for "No presence" (Null-spz-1 and Null-spz-2 pools), one for "High presence" (High-spz pool), and one for "Rare presence" (Rare-spz pool).

Embryos from vitrified vs. fresh oocytes in an oocyte donation program: a comparative morphokinetic analysis.

Objective: To compare the morphokinetic patterns of human embryos originating from vitrified oocytes (VITRI group) with those derived from freshly collected oocytes (CONTROL group) in oocyte donation cycles.

Design: This is a retrospective observational study.

Setting: Embryolab Fertility Clinic, Embryology Lab, Thessaloniki, Greece.

Double vitrification of embryos adversely affects clinical outcomes.

Objective: To evaluate the impact of double embryo vitrification on clinical outcomes.

Methods: This retrospective cohort study included data from January 2013 to March 2021. The study group included women aged 33.

The impact of fresh and frozen testicular tissue quality on embryological and clinical outcomes.

Our ability to predict the potential of testicular spermatozoa to support embryonic development is still limited. Although motility of testicular spermatozoa is associated with embryo development, the impact of morphology and the presence of spermatozoa in the testicular sample has not been previously researched. Moreover, while the majority of data indicate no effect of cryopreservation, there are studies reporting impaired clinical outcomes due to testicular cryopreservation.