Development of Etf-3-specific nanobodies to prevent Ehrlichia infection and LNP-mRNA delivery in cellular and murine models.

Ehrlichia chaffeensis is an obligatory intracellular bacterium that infects monocytes and macrophages and causes human monocytic ehrlichiosis. Ehrlichia translocated factor-3 (Etf-3) is a type IV secretion system effector that binds host-cell ferritin light chain and induces ferritinophagy, thus increasing cellular labile iron pool for Ehrlichia proliferation. To further characterize roles of Etf-3 in Ehrlichia infection, we produced immune libraries of Etf-3-specific nanobodies (Nbs).

Ticked Off: A Case of Disappearing Cell Lines.

Human granulocytic anaplasmosis (HGA) is transmitted by the black-legged tick and presents with fever, thrombocytopenia, leukocytopenia, and elevated transaminases. If left untreated, HGA can progress to hemophagocytic lymphohistiocytosis (HLH), which can be fatal. Here, we discuss a case of a woman diagnosed with anaplasmosis who was treated promptly.

Global prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum coinfection in wild and domesticated animals: A systematic review and meta-analysis.

Background: Both Borrelia burgdorferi (Bb) and Anaplasma phagocytophilum (Ap) can infect humans and animals through tick-borne transmission, resulting in zoonosis. Under certain conditions, human infection can lead to Lyme disease (LD) and human granulocytosis (HGA), whereas infection in animals can cause various acute and non-specific symptoms. The combination of Bb and Ap has been reported to increase the disease severity in infected animals.

Epidemiology of canine ehrlichiosis and molecular characterization of Erhlichia canis in Bangladeshi pet dogs.

Background: Ehrlichia canis, a rickettsial organism, is responsible for causing ehrlichiosis, a tick-borne disease affecting dogs.

Objectives: This study aimed to estimate ehrlichiosis prevalence and identify associated risk factors in pet dogs.

Methods: A total of 246 peripheral blood samples were purposively collected from pet dogs in Dhaka, Mymensingh, and Rajshahi districts between December 2018 and December 2020.

[Equine granulocytic anaplasmosis (EGA): Case description and overview of the epidemiological situation with focus on Germany].

Equine granulocytic anaplasmosis (EGA) is a tick-borne disease caused by (.) . In Central, Northern, Western, and Eastern Europe, ticks of the (.

Transmission risk of vector-borne bacterial diseases (Anaplasma spp. and Ehrlichia canis) in Spain and Portugal.

Background: Ehrlichiosis and anaplasmosis are vector-borne bacterial diseases produced by intracellular rickettsial species of the genus Ehrlichia and Anaplasma. Ehrlichia canis and Anaplasma spp. (A.

Ehrlichia canis in Human and Tick, Italy, 2023.

In August 2023, ehrlichiosis was confirmed in a patient in Italy with a Haemaphysalis punctata tick attached to his neck. Gene sequences of Ehrlichia canis from the tick and the patient were identical, indicating a potential risk for this uncommon infection for persons participating in outdoor activities.

Natural Panola Mountain Ehrlichia infections in cattle in a longitudinal study of Angus beef calves.

Panola Mountain Ehrlichia (PME) is an emerging zoonotic pathogen, transmitted by Amblyomma americanum ticks in the southeastern United States. It is closely related to Ehrlichia ruminantium, the causative agent of heartwater disease. Heartwater disease is an often-fatal illness of ruminant livestock present in Africa and the Caribbean.

Scientometric mapping of the trends, impact, and thematic evolution of scientific production on ehrlichiosis in veterinary medicine.

Background And Aim: This study focuses on the scientific output of ehrlichiosis, a tick-borne disease that affects a variety of animal species, including dogs, cats, and livestock. Therefore, this study aimed to perform a scientometric mapping of the trends, impact, and thematic evolution of scientific production on ehrlichiosis in veterinary medicine.

Materials And Methods: The study design was descriptive and observational, with a quantitative scientometric approach.

Heartland Virus Infection in Elderly Patient Initially Suspected of Having Ehrlichiosis, North Carolina, USA.

We report a patient in North Carolina, USA, with Heartland virus infection whose diagnosis was complicated by previous Ehrlichia chaffeensis infection. We identified E. ewingii-infected and Bourbon virus-infected tick pools at the patient's residence.

Immuno- and expression analysis of immunoreactive proteins.

is the primary etiologic agent of canine monocytic ehrlichiosis, a serious and sometimes fatal hemorrhagic disease of dogs. Diagnosis of infection is often retrospectively confirmed by serologic detection of antibodies by immunofluorescent microscopy. Our laboratory previously identified numerous major immunoreactive proteins with species-specific linear antibody epitopes that are useful for immunodiagnosis of CME.

Pulmonary hypertension with severe sepsis in ehrlichiosis: what you should know before you hike.

Ehrlichiosis is a tickborne illness that can lead to an increased risk of death without appropriate treatment. According to Centers for Disease Control and Prevention, there has been a rise in incidence. We present a case of an immunocompetent patient who reported tick removal after hiking.

Molecular Detection of Ehrlichia ruminantium in Cattle From Different Agro-Ecological Zones of Cameroon: Implication for the Understanding of the Heartwater Epidemiology.

Although Amblyomma variegatum and Ehrlichia ruminantium infections have been reported in cattle from some agro-ecological zones (AEZs) of Cameroon, the transmission patterns of this bacterium seem to vary according to endemic areas and its prevalence as well as that of Heartwater remains not well understood in most sub-Saharan African countries. This study was designed to detect E. ruminantium infections in cattle of four AEZs of Cameroon and to identify areas presenting enzootic stability and those with potentially high risk for Heartwater.

RipE expression correlates with high ATP levels in , which confers resistance during the extracellular stage to facilitate a new cycle of infection.

Ehrlichiosis is a potentially life-threatening disease caused by infection with the obligatory intracellular bacteria species. infection of mice provides an animal model of ehrlichiosis as it recapitulates full-spectrum and lethal ehrlichiosis in humans. The transposon mutant of , which encodes a previously uncharacterized hypothetical protein, is attenuated in both infection and virulence in mice.

invasin AipA interacts with CD13 to elicit Src kinase signaling that promotes infection.

Unlabelled: Host-microbe interactions that facilitate entry into mammalian cells are essential for obligate intracellular bacterial survival and pathogenesis. is an obligate intracellular bacterium that invades neutrophils to cause granulocytic anaplasmosis. The invasin-receptor pairs and signaling events that induce uptake are inadequately defined.

Molecular, epidemiological, and hematological evaluation in infected dogs from an endemic region in Egypt.

Background: Canine monocytic ehrlichiosis (CME) is considered a multisystemic, life-threatening, rickettsial, and tick-borne disease that affects canine species and is caused by . Clinical signs of CME vary from asymptomatic to severe illness with three clinical phases. has the potential to infect humans.

Significance of Anaplasma phagocytophilum, Babesia caballi, and Theileria equi as etiologic agents in horses with clinical manifestations from the metropolitan area of Rio De Janeiro, Brazil.

Equine Piroplasmosis (EP) and Equine Granulocytic Anaplasmosis (EGA) are diseases that affect horses, transmitted by ixodid ticks, causing a nonspecific febrile syndrome. Equine Piroplasmosis is endemic in Brazil, and most horses are in enzootic stability. Serological and molecular studies carried out on horses in Brazil have shown the presence of Anaplasma phagocytophilum, however, the clinical relevance of this infection has not yet been established.

Guillain-Barré syndrome in a 63-year-old patient possibly triggered by ehrlichiosis. Case report.

Guillain-Barré syndrome is an immune-mediated acute demyelinating polyradiculoneuropathy, characterized by progressive flaccid weakness, triggered mainly by respiratory and gastrointestinal infections. We present the case of a 63-year-old male patient with a history of infection, who consulted the internal medicine emergency department for lower back pain and progressive lower limb paresthesia, accompanied by decreased lower limb strength and nerve conduction velocity test, with results compatible with acute demyelinating sensorimotor polyradiculoneuropathy. To the best of our knowledge, this is the first documented case in Honduran medical literature; in our research, no other cases were found in Latin America or Europe.