Investigation of and in Pakistan, with notes on the detection of tickborne Rickettsiales.

There is limited information on the occurrence of and ticks, as well as associated and species in Pakistan. Addressing this knowledge gap, the current study aimed at morphomolecular confirmation of these ticks and molecular assessment of associated Rickettsiales bacteria (, and spp.) in Balochistan, Pakistan.

The sesquiterpene alcohol farnesol mitigates cadmium hepatotoxicity by attenuating oxidative stress and NF-kappaB/NLRP3 inflammasome axis and upregulating PPARgamma in rats.

Farnesol (FAR) is a sesquiterpene alcohol that exists in many fruits and vegetables and possesses promising anti-inflammatory and antioxidant activities. Cadmium (Cd) is an environmental pollutant known for its serious health effects. Liver injury associated with oxidative stress is a hazardous consequence of exposure to Cd.

Exploring the role of noncoding RNAs in cancer diagnosis, prognosis, and precision medicine.

This review article studies the complex field of noncoding RNAs (ncRNAs) in cancer biology, focusing on their potential use as biomarkers and therapeutic targets. NcRNAs include circular RNAs (circRNAs), long noncoding RNAs (lncRNAs), and microRNAs (miRNAs). We discuss how ncRNAs affect gene expression in cancerous cells, the spread of cancer, and metastasis.

Pediatric Pulmonary Arterial Hypertension; is it Possible to Predict its Outcome?

Objectives: To assess the outcome of pediatric pulmonary arterial hypertension (PAH) and to identify the predictors of morbidity and mortality of this progressive disease.

Patients And Methods: This prospective observational cohort study was conducted on consecutive pediatric patients with PAH. Medical history was taken with a grading of the WHO functional class as well as the serum N-terminal pro-BNP (NT pro-BNP), 6 min' walk test (6MWT), and echocardiography at the initial assessment and at follow-up.

An eco-friendly and cost-effective approach for the synthesis of a novel GA@CuO-ZnO nanocomposite: characterization, antimicrobial and anticancer activities.

In this study, a nanocomposite based on copper oxide-zinc oxide nanoparticles and Gum Arabic (GA@CuO-ZnO nanocomposite) was successfully synthesized using green method. Characterization results revealed that the prepared nanocomposite appeared at the nanoscale level, showed excellent dispersion, and formed stable colloidal nano-solutions. The bimetallic GA@CuO-ZnO nanocomposite was evaluated for its anticancer, antibacterial, and antifungal properties.

Potent EGFR/PARP-1 inhibition by spirooxindole-triazole hybrids for targeted liver cancer therapy.

The search for effective anti-cancer therapies has led to the exploration of dual inhibition strategies targeting multiple key molecular pathways. In this study, we aimed to design a novel candidate capable of dual inhibition targeting both EGFR (Epidermal Growth Factor Receptor) and PARP-1 (poly(ADP-ribose)polymerase-1), two crucial proteins implicated in cancer progression and resistance mechanisms. Through molecular hybridization and structure-based drug design approaches, we synthesized a series of compounds based on spirooxindole with triazole scaffolds with the potential for dual EGFR and PARP-1 inhibition.

Syphilis infection prevalence in the Middle East and North Africa: a systematic review and meta-analysis.

Background: Syphilis is a sexually transmitted infection (STI) that can be prevented and effectively treated; yet it continues to be a cause of morbidity and mortality worldwide. There is a limited understanding of the epidemiology of syphilis in the Middle East and North Africa (MENA) region.

Methods: A systematic review conducted up to April 30, 2024 assessed the prevalence of syphilis and followed PRISMA guidelines, without language and date restrictions.

Comparison of 1-Year Clinical Outcomes Between Ticagrelor Versus Clopidogrel in Type 2 Diabetes Patients After Implantation of Small Diameter Stents.

Background: Type 2 diabetes mellitus (T2DM) patients with small-diameter stents (SDS), that are equal to or less than 2.5 mm in diameter, face increased risks of restenosis and complications. This study aimed to evaluate the 1-year follow-up to assess the rate of major adverse cardiac events (MACE) and bleeding risk between ticagrelor and clopidogrel in T2DM patients after SDS implantation.

Development of a Novel Synchronous Spectrofluorimetric Method Coupled With Chemometric Modelling for Ultrasensitive Analysis of Tenofovir and Dolutegravir With Implication for Pharmacokinetic Studies.

This study introduces a novel synchronous spectrofluorimetry coupled with chemometric tools for the determination of tenofovir and dolutegravir antiretroviral drugs. Utilizing partial least squares regression (PLS) fine-tuned by genetic algorithm as variable selection tool, the developed models demonstrate greater sensitivity, cost-effectiveness, and reduced environmental impact compared to traditional HPLC methods. The model's validation was further confirmed using external validation in addition to QC samples as per ICH M10 guidelines, which yielded high accuracy ranged between 94.

Lethal Phenotype and Expansion of the Clinical Spectrum of Biallelic Loss of Function Variant in SENP7 Gene Unveiled by Whole Exome Sequencing.

SUMOylation involves covalent attachment of small ubiquitin-like modifier (SUMO) proteins to specific lysine residues on target proteins and regulates various aspects of their function. Sentrin-specific proteases (SENPs) are key players in both the conjugation reaction of SUMO proteins to their targets and the subsequent deconjugation of SUMO-conjugated substrates. Here, we provide the first comprehensive prenatal description of a lethal syndrome linked to a novel homozygous stop-gain variant in SENP7 c.

Using Microalgae as a Good Motivator of Ossimi Lamb's Health and Growth.

In tropical and subtropical countries like Egypt, sheep breeding faces environmental thermal stress, especially during the summer when air temperature and humidity are very high. Using Microalgae as an alternative feedstuff can significantly improve sheep growth, health and oxidative status. Therefore, this study aimed to evaluate the effect of consuming two different species of microalgae: Spirulina platensis (SP) and Chlorella vulgaris (C.

Fabrication of promising competitive graphene nanocomposite transducer to determine Prucalopride succinate in pharmaceutical formulation and in spiked human biological fluids.

The development of a newly fabricated ion-selective electrode (ISE) solid-contacted type for the determination of prucalopride succinate represents a significant advancement in analytical chemistry, particularly in the context of green chemistry principles. The optimization process involved numerous trials to ensure the selection of a cation exchanger and ionophore that offer high sensitivity and selectivity for prucalopride succinate. Through these optimization trials, sodium tetrakis was identified as the most suitable cation exchanger, while calix [8] arene demonstrated the highest affinity towards prucalopride succinate as the ionophore.

Therapeutic extraction of second molars in orthodontics: a scoping review.

Objective: To review the literature to identify the present evidence on the extraction of second molars in orthodontics.

Materials & Method: A search of the MEDLINE/PubMed, Scopus, Web of Science™, and ProQuest databases for full-text articles was done on March 5, 2024. The search went back till the 1st of January 1991 and was limited to articles in English.

Effect of negotiation skills training program on head nurses' knowledge and behavior.

Background: Negotiating is a common occurrence and a significant part of everyday tasks for head nurses. The ability of the head nurse to effectively negotiate is a crucial management tool for work management in healthcare facilities.

Aim: The present study aimed to assess the effect of negotiation skills training program on head nurses' knowledge and behavior.

Chitosan nano-formulation enhances stability and bactericidal activity of the lytic phage HK6.

Background: Successful treatment of pathogenic bacteria like Enterobacter Cloacae with bacteriophage (phage) counteract some hindrance such as phage stability and immunological clearance. Our research is focused on the encapsulation of phage HK6 within chitosan nanoparticles.

Result: Encapsulation significantly improves stability, efficacy, and delivery of phages.

Trueness of tooth modified scan bodies as a novel technique for edentulous full arch implant supported dental prosthesis: an in vivo prospective comparative study.

Objective: This study aimed to evaluate the clinical performance (degree of trueness) of a novel scan body "tooth-modified Scan body" (TMSB)& conventional scan body (CSB) in implant-supported full arch screw retained cases.

Methods: Seven edentulous arches (two maxillae, five mandibles) in 6 patients were rehabilitated with monolithic zirconia screw-retained implant prostheses supported by 4 (n = 1) and 5 implants (n = 6) for a total amount of 34 implants. Implant locations were scanned by intra-oral scanner (IOS) using two types of scan bodies, conventional scan bodies (CSB) in group (1) and tooth-modified scan bodies (TMSB) in group (2).

Effect of dietary supplementation of Lawsonia inermis and Acacia nilotica extract on growth performance, intestinal histopathology, and antioxidant status of broiler chickens challenged with coccidiosis.

Avian coccidiosis is one of the many disorders that seriously harm birds' digestive systems. Nowadays the light is shed on using Phytochemical/herbal medicines as alternative natural anti-coccidial chemical-free standards. Consequently, this study aimed to investigate the impact of lawsonia inermis powder (LIP), and Acacia nilotica aqueous extract (ANAE), on growth performance, serum biochemical, antioxidant status, cytokine biomarkers, total oocyst count and intestinal histopathology of broiler chickens challenged with coccidiosis.

Cessation of thumb/finger sucking habit in children using electronic habit reminder versus palatal crib: a randomized clinical pilot study.

Background: There are different intraoral appliances for cessation of thumb/finger sucking habit, but they have many disadvantages and to overcome it, extra oral appliances with colourful and attractive shape were developed. Electronic habit reminder in the form of wristwatch with alarming sound was assessed in cessation of thumb/finger sucking habit in children versus palatal crib after 6 and 9 months.

Methods: This study is a randomized clinical pilot study, with allocation ratio 1:1 parallel group.

Automatic image generation and stage prediction of breast cancer immunobiological through a proposed IHC-GAN model.

Invasive breast cancer diagnosis and treatment planning require an accurate assessment of human epidermal growth factor receptor 2 (HER2) expression levels. While immunohistochemical techniques (IHC) are the gold standard for HER2 evaluation, their implementation can be resource-intensive and costly. To reduce these obstacles and expedite the procedure, we present an efficient deep-learning model that generates high-quality IHC-stained images directly from Hematoxylin and Eosin (H&E) stained images.

Pentraxin 3: a novel biomarker in pediatric central nervous system infections.

Background: Pediatric CNS infections have been identified as a global health problem, associated with an increased death rate and fatal consequences. Pentraxin 3 (PTX3) is an acute-phase mediator that increases in body fluids and plasma throughout inflammation. Our study was designed to assess the diagnostic and prognostic value of cerebrospinal fluid (CSF) PTX3 levels in pediatric patients with different central nervous system (CNS) infections.

Floral diversity and conservation status of vascular plants in arid mountainous areas.

Background: The destructive human activities, encroachment of natural habitats, and hyperarid climate threaten the wild flora of the unprotected mountainous areas facing the Gulf of Suez, Egypt. So, this study aims to revise and give an updated systematic status of the flowering plants growing there to conserve and utilize valuable biodiversity.

Results: This study showed the presence of 136 species, including 7 sub-species of vascular plants, 12 species of monocots, and 124 species dicots belonged to 98 genera and 37 families.

Potential mutagenicity of aflatoxin B1 in Egyptian spices.

The current study aimed to detect the mutagenic impacts of aflatoxin B1 (AFB1), which is produced by Aspergillus group fungi, via a high-plant genotoxicity test. Different durations of treatment (3 h, 6 h, and 12 h) were used to treat the Vicia faba root tips with varying concentrations of Aflatoxin B1 (AFB1) following the approved protocol for plant assays published by the International Program on Chemical Safety (IPCS) and the World Health Organization (WHO). The data obtained indicated that AFB1 not only has the ability to induce various alterations in the process of mitosis, ranging from increasing to decreasing mitotic and phase indices but also leads to many mitotic aberrations.

Fusarium oxysporum assisted green synthesis of small-sized silver nanoparticles for high antibacterial, and photocatalytic decolorization performances.

Background: Novel platforms using nanotechnology-based medicines have exponentially increased in our daily lives. The unique characteristics of metal oxide and noble metals nanoparticles make them suitable for different fields including antimicrobial agents, cosmetics, textiles, wound dressings, and anticancer drug carriers.

Methods: This study focuses on the biosynthesis of small-sized SNPs using exo-metabolites of Fusarium oxysporum via bioprocess optimization using Plackett-Burman (PBD) and central composite designs (CCD) while evaluating their multifaceted bioactivities.

Eversion cruroplasty and collar overwrap: a novel hybrid approach for refractory gastroesophageal reflux disease in children, with assessment of mid-term outcomes.

Background: Surgical fundoplication remains integral in managing gastroesophageal reflux disease (GERD) by addressing gastroesophageal valve incompetence. This study introduces a novel hybrid approach, the Eversion Cruroplasty and Collar Overwrap (ECCO) procedure, aiming to combine benefits of conventional partial wrapping and posteromedial cardiopexy, considering gastric fundus anatomical peculiarities as an anti-reflux barrier.

Methods: A retrospective analysis of pediatric patients presenting with refractory GERD from 2021 to 2023 was conducted.

Biosorbent silver nanoparticles decorated coffee-ground waste composite for cleaning water and antimicrobial applications.

A sustainable biosorbent, silver nanoparticles-decorated coffee-ground waste (CWAg), was synthesized through a simple in-situ reduction method. CWAg is extensively characterized via SEM-EDX, PZC, FTIR, XRD, HR-TEM, and XPS analyses. The biosorbent was tested to remove chromium (Cr(VI)) and methylene blue (MB) from wastewater, and its antibacterial properties was evaluated.