Role of the commissural inhibitory system in vestibular compensation in the rat.

We investigated the role of the vestibular commissural inhibitory system in vestibular compensation (VC, the behavioural recovery that follows unilateral vestibular loss), using in vivo microdialysis to measure GABA levels in the bilateral medial vestibular nucleus (MVN) at various times after unilateral labyrinthectomy (UL). Immediately after UL, in close correlation with the appearance of the characteristic oculomotor and postural symptoms, there is a marked increase in GABA release in the ipsi-lesional MVN. This is not prevented by bilateral flocculectomy, indicating that it is due to hyperactivity of vestibular commissural inhibitory neurones.

The lectin Dolichos biflorus agglutinin is a sensitive indicator of branching morphogenetic activity in the developing mouse metanephric collecting duct system.

The urine collecting duct system of the metanephric kidney develops by growth and branching morphogenesis of an unbranched progenitor tubule, the ureteric bud. Bud branching is mainly dichotomous and new branches form from existing branch tips, which are also the main sites of cell proliferation in the system. This behaviour, and the fact that some genes (e.

A role for microfilament-based contraction in branching morphogenesis of the ureteric bud.

Background: Branching morphogenesis of the ureteric bud/collecting duct epithelium is an important feature of kidney development. Recent work has identified many transcription factors and paracrine signaling molecules that regulate branching, but the physical mechanisms by which these signals act remain largely unknown. The actin cytoskeleton is a common component of mechanisms of morphogenesis.

Watching tubules glow and branch.

Branching morphogenesis is an important mechanism of animal development yet, until recently, most details about this highly dynamic process have had to be inferred from fixed tissues. Several groups have now developed transgenic animals in which branching tubules express fluorescent proteins, enabling their morphogenesis to be studied dynamically using time-lapse microscopy. The results have shown that branch emergence is highly variable, that sprouting tracheae and blood vessels guide themselves by filopodial projections, that branching morphogenesis can involve highly ordered cell rearrangements, and that branches are subject to intense remodelling.

Structural determinants of heparan sulphate modulation of GDNF signalling.

Glial cell line-derived neurotrophic factor (GDNF) has many functions including regulation of kidney morphogenesis and of neuron growth and survival in the enteric, sensory and central nervous systems. Reports of GDNF being used against Parkinson's disease in human patients have sparked intense clinical interest in GDNF signalling. We recently showed that GDNF signalling requires cell surface heparan sulphate glycosaminoglycans (Barnett et al.