Polymorphic Loci of Xenobiotic Biotransformation Genes in Accumulated Mercury Pollution Liquidators and in Workers with Chronic Mercury Vapors Exposure.

The allele and genotype frequencies of the polymorphic loci CYP1A1 (rs1048943), GSTP1 (rs1695 and rs1138272), GSTM1, and GSTT1 genes were studied in 517 men: in 389 accumulated mercury pollution liquidators (207 firefighters of the Ministry of the Russian Federation for Civil Defence, Emergencies and Elimination of Consequences of Natural Disasters and 182 employees of the Federal Environmental Operator) and 128 former workers (82 patients in the delayed period of chronic mercury intoxication and 46 individuals contacted with mercury and had no chronic mercury intoxication). We found differences in the frequencies of AA and AG genotypes in groups of former workers (χ=6.96, p=0.

Altered serum levels of neuronal proteins and antibodies to them in occupational diseases of the nervous system.

Aclinical and immunological examination of men with occupational pathology, including vibration disease (VD), occupational sensorineural hearing loss (SHL), and chronic mercury intoxication (CMI), was carried out. The comparison group consisted of men comparable in age and total work experience. Serum concentrations of neurotrophins (S100β, MBP, BDNF) and antibodies (ABs) to S100β and MBP proteins were determined by enzyme-linked immunosorbent assay.

Tissue Reaction to Low-Density Polyacrylamide Gel as a Carrier for Microimplants in the Adipose Fin of Rainbow Trout.

The implantation of optical sensors is a promising method for monitoring physiological parameters of organisms in vivo. For this, suitable hydrogels are required that can provide a biocompatible interface with the organism's tissues. Amorphous hydrogel is advantageous for administration in animal organs due to its ease of injection compared to resilient analogs.

Effects of Daily Peat Smoke Exposure on Present and Next Generations.

This study aimed to follow the neurotoxic effect of peat smoke on adult outbred rats and its influence on central nervous system (CNS) parameters in first-generation offspring. Under experimental conditions, exposure to peat smoke was carried out on adult male Wistar rats for 24 h. After the end of the exposure, an open field test (OFT), electroencephalography (EEG), and histological analysis of the testes and brains of smoke-exposed males were performed, after which they were mated with intact females to obtain F1 offspring.

Durability of Implanted Low-Density Polyacrylamide Hydrogel Used as a Scaffold for Microencapsulated Molecular Probes inside Small Fish.

Implantable sensors based on shaped biocompatible hydrogels are now being extensively developed for various physiological tasks, but they are usually difficult to implant into small animals. In this study, we tested the long-term in vivo functionality of pH-sensitive implants based on amorphous 2.7% polyacrylamide hydrogel with the microencapsulated fluorescent probe SNARF-1.

The Analysis of Acute and Subacute Toxicity of Silver Selenide Nanoparticles Encapsulated in Arabinogalactan Polymer Matrix.

The acute and subacute toxicity of a newly synthesized silver selenide nanoparticles encapsulated in a natural polymeric matrix of arabinogalactan study has been studied. The nanocomposite is a promising material for the design of diagnostic and therapeutic drugs. It can also be used for the preparation of fluorescent labels and in thermal oncotherapy.

Comparative Assessment of Silver Nanocomposites' Biological Effects on the Natural and Synthetic Matrix.

The aim of our investigation was to make a comparative assessment of the biological effects of silver nanoparticles encapsulated in a natural and synthetic polymer matrix. We carried out a comparative assessment of the biological effect of silver nanocomposites on natural (arabinogalactan) and synthetic (poly-1-vinyl-1,2,4-triazole) matrices. We used 144 three-month-old white outbred male rats, which were divided into six groups.

Alterations in CNS Functions and DNA Methylation in Rats after 24 h Exposure to Peat Smoke.

The use of a developed experimental model of a natural fire made it possible to assess the consequences of 24 h exposure to peat combustion products in albino rats. Peat smoke exposure leads to behavioral disturbances in rats, characterized by an increase in locomotor activity and an increased level of anxiety. Indicators of brain bioelectrical activity of the exposed animals supported the state of anxiety and psychoemotional stress.

[Features of the nutritional status and nutrition of children with autistic disorders].

In recent years, there has been a steady increase in the number of children with autism spectrum disorders (ASD). According to the Ministry of Health of Russia, the incidence of ASD in children under 2 years of age is 5:10 000, at the age of 4 years - 18:10 000. ASD is a complex multisystem disease that affects metabolic and neurobiological processes.

Analysis of HSPA1B (+1267A>G) Genetic Polymorphism in Patients with Vibration Disease in Combination with Metabolic Syndrome.

In 167 men with vibration syndrome (VS), allele and genotype frequencies of HSPA1B (+1267A>G) genetic polymorphism (rs1061581) were studied depending on the type of vibration exposure and on the presence or absence of metabolic syndrome (MetS). The examined subjects were divided into two groups: VS patients (n=80) and VS+MetS patients (n=87). The differences in the lipid profiles between groups were revealed against the background of the lack of distinctions in the age of patients for A/G and G/G genotypes carriers.

[Risk assessment for public health associated with nitrate content in crop products].

The increasing need of the population for crop products (СP) makes continuous monitoring of nitrate content an increasingly urgent task. The accumulation of multidirectional data on the effect of nitrates on the state of the body requires further studies to assess the alimentary risk caused by their admission to the population of various regions. of the work was to study the levels of nitrate contamination of CP and assess the associated risk to the health of children and adults in the Baikal Region.

[Assessment of the population and subpopulation composition of lymphocytes and their relationship in patients with sensorineural hearing loss].

Unlabelled: Study of the role of the phenotypic composition of lymphocytes, shifts in which can cause the development of various pathological processes, including sensorineural hearing loss, is an important area of modern research. Purpose of the study. To study the population and subpopulation composition of lymphocytes and their intercellular cooperation in persons with occupational sensorineural hearing loss.

Paternal Biomass Smoke Exposure in Rats Produces Behavioral and Cognitive Alterations in the Offspring.

Particular concern at the present stage is the health effects of wildfires' smoke. The aim of the study was to determine the impact of paternal biomass-smoke exposure on offspring's behavior and cognitive abilities. Male rats were exposed to biomass smoke for four hours/day, five days/week, for four weeks.

[Functional activity of the brain depending on the degree of severity occupational noise-induced hearing loss].

Unlabelled: Research objective identification of the peculiarities of indicators of audiological examination, registration of somatosensory induced potentials (SSSP), level of constant potential (LCP) and neuropsychological testing in persons of flight composition (LLS) of civil aviation exposed to intra-cabin noise, depending on the degree of expression of professional neurosensory noise (ONIHL).

Patients And Methods: Thresholds of acoustical sensitivity, levels of perception of shepotny, informal conversation, UPP, characteristic of SSVP, neuropsychological features at 45 patients with easy degree of PNST (LONIHL) and at 50 - with moderate degree of ONIHL (UONIHL) are studied.

Results: LCP in the left frontal (Fs), central dark (Pz) leads increased in persons with ONIHL, and the inter-peak interval of the N13-N20 characterizing the central conduct time increased.

Synthesis and comparative assessment of antiradical activity, toxicity, and biodistribution of κ-carrageenan-capped selenium nanoparticles of different size: in vivo and in vitro study.

In the present study, water-soluble hybrid selenium-containing nanocomposites have been synthesised via soft oxidation of selenide-anions, preliminarily generated from elemental bulk-selenium in the base-reduction system 'NH-NaOH'. The nanocomposites obtained consist of SeNPs (4.6-24.

[Comparative characteristic of cognitive impairment at alcoholic and discirculatory encephalopathy].

Objective: Identification of features of cognitive impairment at patients with alcoholic and discirculatory encephalopathy.

Material And Methods: 30 patients with the alcoholic encephalopathy (AE) and 32 - with discirculatory encephalopathy (DE) participated in research. To patients conducted clinical examination, a computer electroencephalography with determination of cognitive evoked potentials, neuropsychological testing.

CYP3A Polymorphism and Chronic Mercury Intoxication.

We analyzed the relationship between polymorphic loci of CYP3A genes (CYP3A4 (rs2740574), CYP3A5 (rs776746) and CYP3A7 (rs2257401)) with the development of chronic mercury intoxication. Of 170 men examined, 120 were workers chronically exposed to mercury vapors and 50 were carriers of GG-HSPA1B (+1267A/G) genotype associated with chronic mercury intoxication. Urinary content of 4-hydroxyantipyrine (4-HAP) generated in the reaction predominantly catalyzed by CYP3A4/CYP3A5 was studied in workers without chronic mercury intoxication (group 1, N=46) and patients in the delayed period of chronic mercury intoxication (group 2, N=74) depending on the genotypes of CYP3A4 and CYP3A5.

Impact of AhRR (565C > G) polymorphism on dioxin dependent CYP1A2 induction.

The current study uses the metabolic probe, antipyrine, and AhRR transcript expression (qRT-PCR) to examine the impact of the AhRR (565C > G or Pro185Ala, rs2292596) genetic polymorphism upon CYP1A2 inducibility in an established cohort of male firefighters with exposure to dioxin-like chemicals. The lipid adjusted concentrations of 29 dioxin and dioxin-like congeners were measured in serum. Possession of the G allele (CG and GG genotypes) was correlated with high expression AhRR transcript and lower CYP1A2 induction than found in individuals homozygous for CC.

Dynamics of Excretion of Thiodiacetic Acid into Urine in Polyvinyl Chloride Production Workers.

Background: Thiodiacetic acid (TDAA) is the main metabolite of vinyl chloride (VC) and 1,2-dichloroethane (EDC) and its urinary level is correlated with the level of exposure to these chemicals.

Objective: To study dynamics of the excretion of TDAA into urine of polyvinyl chloride (PVC) production workers.

Methods: The study sample consisted of 65 workers of VC and PVC divisions with various time intervals following exposure to the chemicals, 10 shift workers from PVC division, and 34 workers not exposed to the chemicals (control group).

Contribution of Fetal Programming in the Formation of Cognitive Impairments Induced by Lead Poisoning in White Rats.

The contribution of prenatal hypoxic damage to the CNS to the formation of high sensitivity of the body to lead acetate was studied. Prenatal fetal hypoxia was modeled by the administration of sodium nitrite in doses of 5, 25, and 50 mg/kg to pregnant female rats. Cognitive capacities of mature offspring were evaluated in the radial maze test and Morris water maze test.

[Features of actual nutrition of the adult population in Republic of Buryatia in modern conditions].

Evaluation of the actual nutrition of the population of individual regions allows the development of targeted practical measures to improve nutrition, the structure of food consumption and appropriate recommendations to ensure optimal development and health status of a person. In order to study the nutrition of the adult population of the Republic of Buryatia, a retrospective analysis of the feeding structure data was conducted according to the data of the territorial department of the Federal State Statistics Service for the Republic of Buryatia, and the results obtained in the course of the questionnaire survey of 426 people (chosen by selective method with observance of representativeness) were analyzed. The data are presented obtained on the results of comparison with physiological needs in energy and nutrients for men and women in the most employable age of 30-39 years, having the third group of labor intensity.

[The case of the fulminant course of amyotrophic lateral sclerosis].

The authors report the case of a lightning-fast course of amyotrophic lateral sclerosis (ALS) in a 49-year female patient. The diagnosis was based on complaints, clinical picture, electroneuromyography and tomography of the cervical spine. The nature of the course of the disease was fulminant, since 6 months passed from the onset of the first symptoms to the pronounced process, and then to the fatal outcome.

[Interrelation of rates of a biological aging with neurophysiological features at patients with professional neurointoxications].

In the hospital conducted studying of functional activity of a brain of patients with professional neurointoxications for the purpose of detection of neurophysiological features of formation of the accelerated rates of biological aging at the working, being affected neurotoxicant is carried out. During the research it is shown that rates of aging at patients with professional neurointoxications on average in 85% of cases have premature character. The general signs of defeat of structures of a brain at professional intoxication are defined by mercury irrespective of a disease stage at the accelerated biological aging.

HSP70 (HSPA1) polymorphisms in former workers with chronic mercury vapor exposure.

Objectives: To investigate 4 loci of 3 HSP70 genes in caustic soda production plant former workers, who have been exposed to metallic mercury vapors for a long time, and including numerous cases of chronic mercury intoxication (CMI).

Material And Methods: Polymorphisms in HSP70 gene family members (HSP1A1 (+190G/C, rs1043618), HSPA1B (+1267A/G and +2074G/C, rs1061581) and HSP1AL (+2437T/C, rs2227956)) genes were studied among 120 male workers involved in caustic soda production by mercury electrolysis at 2 plants in Eastern Siberia. These subjects had been chronically exposed to metallic mercury vapors for > 5 years and divided into 3 groups based on the occurrence and time of the CMI diagnosis, or absence of this disease.

Impact of dioxins on antipyrine metabolism in firefighters.

Antipyrine (AP) metabolism was used to assess factors associated with the activity of hepatic oxidative enzymes in firefighters. Emphasis was placed on 3-hydroxymethylantipyrine (3HMAP), the metabolite with the greatest dependence on dioxin-inducible cytochrome P4501A2 (CYP1A2) activity. AP urinary metabolites were measured by HPLC in 38 male subjects from Eastern Siberia.