Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L.

Glyphosate (Gly) is a widely used herbicide for weed control in agriculture, but it can also adversely affect crops by impairing growth, reducing yield, and disrupting nutrient uptake, while inducing toxicity. Therefore, adopting integrated eco-friendly approaches and understanding the mechanisms of glyphosate tolerance in plants is crucial, as these areas remain underexplored. This study provides proteome insights into Si-mediated improvement of Gly-toxicity tolerance in Brassica napus.

Superconjugated Anthraquinone Carbonyl-Based Covalent Organic Framework as Anode Material for High-Performance Aqueous Ammonium-Ion Batteries.

Covalent organic frameworks (COFs) have recently been emerged as promising anode candidates for aqueous ammonium ion batteries (AAIBs), but suitable COFs need to be developed and the energy storage mechanisms need to be further investigated. Here we report an anthraquinone carbonyl-containing COF as anode for AAIBs, which exhibits a high specific capacity of 141 mAh g at 0.1 A g and good cycling stability with 90 % retention after 8000 cycles at 6 A g.

Taming vision transformers for clinical laryngoscopy assessment.

Objective: Laryngoscopy, essential for diagnosing laryngeal cancer (LCA), faces challenges due to high inter-observer variability and the reliance on endoscopist expertise. Distinguishing precancerous from early-stage cancerous lesions is particularly challenging, even for experienced practitioners, given their similar appearances. This study aims to enhance laryngoscopic image analysis to improve early screening/detection of cancer or precancerous conditions.

Degradation mechanism of PGNa by the immobilized degrading enzymes from magnetic fermentation residue biochar.

The development of a method to efficiently remove high concentrations of penicillin G sodium (PGNa) from the environment is important for human and animal health and safety. In this study, the degradative enzymes were immobilized by adsorption using biochar from penicillin fermentation waste residue, which could efficiently remove PGNa (900 mg/L) from an aqueous solution, with a removal rate of 99.84 % within 20 min.

Waterbird community response to wetland and climate changes in the Liaohe River Estuary wetlands, China.

The Liaohe River Estuary (LRE) wetland is a critical stopover on the East Asian-Australasian Flyway (EAAF), vital to coastal ecological balance and biodiversity. However, the drivers of changes in waterbird diversity remain unclear. This study utilised random forests to produce reliable time-series wetland mapping from 2010 to 2023.

An all-in-one PEGylated NIR-II conjugated polymer for high-resolution blood circulation imaging and photothermal immunotherapy.

Fluorescence imaging in the second near-infrared window (NIR-II) has shown tremendous potential for in vivo monitoring of biological processes, offering high spatial resolution and real-time imaging capabilities. Conjugated polymers, commonly used as photothermal agents (PTAs) in photothermal therapy, have emerged as promising candidates for NIR-II imaging. However, their imaging efficiency is compromised by aggregation, which arises from strong π-π stacking interactions between their extended π-conjugated backbones.

Comparative metagenomics reveals the metabolic flexibility of coastal prokaryotic microbiomes contributing to lignin degradation.

Coastal wetlands are rich in terrestrial organic carbon. Recent studies suggest that microbial consortia play a role in lignin degradation in coastal wetlands, where lignin turnover rates are likely underestimated. However, the metabolic potentials of these consortia remain elusive.

Chinese Registry of Rheumatoid Arthritis (CREDIT) VI: Temporal Trends in Patients With Early Rheumatoid Arthritis and Moderate-To-Severe Disease Activity - A Multicenter Cohort Study of Treatment Strategies and Outcomes.

Aim: The continuous update of international guidelines and enhanced availability of biological disease-modifying antirheumatic drugs (bDMARDs) and targeted synthetic DMARDs (tsDMARDs) have made a significant impact on the diagnosis and treatment of early rheumatoid arthritis (ERA). This study aims to systematically evaluate the current treatment strategies and outcomes within a large-scale cohort of patients with ERA.

Methods: Data from the Chinese Registry of Rheumatoid Arthritis (CREDIT), a large multicenter Chinese registry of RA, were collected to analyze temporal trends in clinical profiles, therapeutic strategies, and treatment outcomes among patients with ERA.

The serum level of sclerostin decreases in radiographic axial spondyloarthritis patients with fatty lesions.

Background: Currently, the pathophysiology of new bone formation in radiographic axial spondyloarthritis (r-axSpA) remains unclear. Cellular elements and their secreted bone turnover markers might be one of the underlying mechanisms that drive the new bone formation. Our study aimed to investigate the role of bone turnover markers in r-axSpA patients with fatty lesions.

The learning curve for laparoscopic-assisted single-port mediastinoscopic esophagectomy: an analysis of proficiency.

Background: Laparoscopic-assisted single-port mediastinoscopic esophagectomy is a safe and effective emerging minimally invasive esophagectomy, but little has been reported about the learning curve for this technology. The goal of the study was to determine the number of procedures to achieve different levels of proficiency on the learning curve.

Methods: This study retrospectively analyzed data from consecutive surgeries performed by the same surgeon at the same center from 2016 to 2021.

Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration.

This study delved into the molecular mechanisms underlying mechanical stress-induced intervertebral disc degeneration (msi-IDD) through single-cell and high-throughput transcriptome sequencing in mouse models and patient samples. Results exhibited an upsurge in macrophage presence in msi-IDD intervertebral disc (IVD) tissues, with secreted phosphoprotein 1 (SPP1) identified as a pivotal driver exacerbating degeneration via the protein kinase RNA-like endoplasmic reticulum kinase/ activating transcription factor 4/ interleukin-10 (PERK/ATF4/IL-10) signaling axis. Inhibition of SPP1 demonstrated promising outcomes in mitigating msi-IDD progression in both in vitro and in vivo models.

Non-metallic iodine single-atom catalysts with optimized electronic structures for efficient Fenton-like reactions.

In this study, we introduce a highly effective non-metallic iodine single-atom catalyst (SAC), referred to as I-NC, which is strategically confined within a nitrogen-doped carbon (NC) scaffold. This configuration features a distinctive C-I coordination that optimizes the electronic structure of the nitrogen-adjacent carbon sites. As a result, this arrangement enhances electron transfer from peroxymonosulfate (PMS) to the active sites, particularly the electron-deficient carbon.

Shellfish and shorebirds from the East-Asian Australian flyway as bioindicators for unknown per- and polyfluoroalkyl substances using the total oxidizable precursor assay.

Per- and polyfluoroalkyl substances (PFAS) have gained significant global attention due to their extensive industrial use and harmful effects on various organisms. Among these, perfluoroalkyl acids (PFAAs) are well-studied, but their diverse precursors remain challenging to monitor. The Total Oxidizable Precursor (TOP) assay offers a powerful approach to converting these precursors into detectable PFAAs.

Molecular mechanisms of cis-oxygen bridge neonicotinoids to Apis mellifera Linnaeus chemosensory protein: Surface plasmon resonance, multiple spectroscopy techniques, and molecular modeling.

Honeybees, essential pollinators for maintaining biodiversity, are experiencing a sharp population decline, which has become a pressing environmental concern. Among the factors implicated in this decline, neonicotinoid pesticides, particularly those belonging to the fourth generation, have been the focus of extensive scrutiny due to their potential risks to honeybees. This study investigates the molecular basis of these risks by examining the binding interactions between Apis mellifera L.

Ultrafast Water Purification by Template-Free Nanoconfined Catalysts Derived from Municipal Sludge.

Nanoconfinement at the interface of heterogeneous Fenton-like catalysts offers promising avenues for advancing oxidation processes in water purification. Herein, we introduce a template-free strategy for synthesizing nanoconfined catalysts from municipal sludge (S-NCCs), specifically engineered to optimize reactive oxygen species (ROS) generation and utilization for rapid pollutant degradation. Using selective hydrofluoric acid corrosion, we create an architecture that confines atomically dispersed Fe centers within a micro-mesoporous carbon matrix in situ.

Identification of the Novel HLA-A*02:1098 Allele by Sanger Dideoxy Nucleotide Sequencing in a Chinese Individual.

The HLA-A*02:1098 allele differs from HLA-A*02:07:01:01 by a single non-synonymous nucleotide change in exon 3.

Hemodynamic Evaluation of Intra-Aortic Dual-Balloon Pump Based on Fluid-Structure Interaction.

The intra-aortic balloon pump (IABP) is a widely-used mechanical circulatory support device that enhances hemodynamics in patients with heart conditions. Although the IABP is a common clinical tool, its effectiveness in enhancing outcomes for patients with acute myocardial infarction and cardiogenic shock remains disputed. This study aimed to assess the effectiveness of intra-aortic dual-balloon pump (IADBP) and its impact on aortic hemodynamics compared with an IABP.

Rapid Generation of Reverse Genetics Systems for Coronavirus Research and High-Throughput Antiviral Screening Using Gibson DNA Assembly.

Coronaviruses (CoVs) pose a significant threat to human health, as demonstrated by the COVID-19 pandemic. The large size of the CoV genome (around 30 kb) represents a major obstacle to the development of reverse genetics systems, which are invaluable for basic research and antiviral drug screening. In this study, we established a rapid and convenient method for generating reverse genetic systems for various CoVs using a bacterial artificial chromosome (BAC) vector and Gibson DNA assembly.

A Paradigm of Computer Vision and Deep Learning Empowers the Strain Screening and Bioprocess Detection.

High-performance strain and corresponding fermentation process are essential for achieving efficient biomanufacturing. However, conventional offline detection methods for products are cumbersome and less stable, hindering the "Test" module in the operation of "Design-Build-Test-Learn" cycle for strain screening and fermentation process optimization. This study proposed and validated an innovative research paradigm combining computer vision with deep learning to facilitate efficient strain selection and effective fermentation process optimization.

Efficacy of preoperative pulmonary rehabilitation in lung cancer patients: a systematic review and meta-analysis of randomized controlled trials.

Although previous studies have shown that preoperative pulmonary rehabilitation training may improve postoperative prognosis in patients with lung cancer, the literature included in the existing meta-analysis is highly heterogeneous and lacks effective subgroup analysis. Therefore, an updated meta-analysis is needed to integrate the latest published randomized controlled clinical trials (RCT). This updated analysis was performed to identify the clinical effects of preoperative pulmonary rehabilitation on physical rehabilitation (lung function, activity endurance, and dyspnea), psychological rehabilitation, quality of life, length of hospital stay, and postoperative pulmonary complications in patients with lung cancer.

Exploring early postoperative core symptoms in Chinese patients with primary liver cancer: a cross-sectional and longitudinal network analysis.

Background: Patients with primary liver cancer (PLC) experience a range of symptoms in the early postoperative period. Symptoms include cancer-related symptoms and adverse effects of treatment. Exploring the core symptoms and their dynamics in the early post-hepatectomy patients may help provide better symptom management programs.

Exploring the coupling coordination relationship and obstacle factors of rural revitalization, new-type urbanization, and digital economy in China.

The digital economy injects new vitality into rural revitalization and new-type urbanization to achieve rural industrial transformation, while the countryside and the city provide the soil for the development of the digital economy. This research establishes the rural revitalization (RR), new-type urbanization (NU), and digital economy (DE) system and uses the coupled coordination degree (CCD) model and obstacle degree (OD) model to study the spatiotemporal evolution characteristics and obstacle factors of the composite system in China from 2011 to 2021. The result showed that: (1) the comprehensive development level of the composite systems is on an upward trend year by year, but still shows a low-quality state; (2) the CCD of China's provinces shows a spatial evolution pattern of high in the east and low in the west; (3) The obstacle factors of the RR, NU, DE subsystem are mainly involved the number of rural doctors and health workers, local financial income per capita and science and technology expenditure, and the digital finance coverage breadth index.

Studies on the treatment of anaerobically digested sludge by white-rot fungi: evaluation of the effect of Phanerochaete chrysosporium and Trametes versicolor.

Background: The composition of anaerobically digested sludge is inherently complex, enriched with structurally complex organic compounds and nitrogenous constituents, which are refractory to biodegradation. These characteristics limit the subsequent rational utilization of resources from anaerobically digested sludge. White-rot fungi (WRF) have garnered significant research interest due to their exceptional capacity to degrade complex and recalcitrant organic pollutants.

Physical activity, Vitamin D, and all-cause/cardiovascular mortality: a prospective study in older Chinese adults.

Background: Physical activity was associated with the risk of all-cause and cardiovascular mortality. However, little is known about older adults, especially those aged over 80 years.

Methods: 2863 older adults in the Chinese Longitudinal Healthy Longevity Survey (CLHLS) were enrolled.

Taming Highly Enolizable Aldehydes via Enzyme Catalysis for Enantiocomplementary Construction of β-Hydroxyphosphonates.

Taming highly enolizable aldehydes for catalytic asymmetric C-C coupling with nucleophiles remains an elusive challenge compared to widely explored simple alkyl or aryl aldehydes. Herein, we use ThDP-dependent enzymes to realize the direct C-C coupling of highly enolizable 2-phosphonate aldehydes with in situ-generated dynamically reversible nucleophiles (acyl anions). Unlike NHC-mediated reactions that yield complex mixtures of multiple adducts, our enzymatic process selectively produces biologically active β-hydroxy phosphonates with high yields (up to 95%) and excellent enantioselectivities (up to 99% ee).