[Design and evaluation of a liquid chromatographic column oven with adaptive and precise temperature control].

High performance liquid chromatography (HPLC) is a key analytical technique that is used in a number of fields. Improving the separation efficiency, stability, and universality of HPLC has been a continuing analytical-chemistry focus. In chromatographic separation, factors such as the composition and ratio of the mobile phase, the type of stationary phase, and the dimensions of the chromatographic column significantly affect the separation efficiency.

Template-free synthesis of single-crystal SrTiO nanocages for photocatalytic overall water splitting.

In this study, we present a novel approach to achieve the template-free fabrication of nanocage-shaped SrTiO (N-STO) single crystals molten salt flux treatment. Systematic characterizations demonstrate the high crystallinity and low defect density of N-STO. The N-STO single crystals enable overall water splitting (OWS) with hydrogen and oxygen evolution rates of 100.

W-mediated electron accumulation in Ru-O-W motifs enables ultra-stable oxygen evolution reaction in acid.

The development of efficient and durable oxygen evolution reaction (OER) catalysts is crucial for advancing proton exchange membrane water electrolysis (PEMWE) technology, especially in the pursuit of non-iridium alternatives. Herein, we report a Zn, W co-doping Ru3Zn0.85W0.

Long-Term Care Insurance and Health Inequality: Evidence From China.

This study examined the relationship between the Chinese Long-Term Care Insurance (LTCI) programme and health inequality among older adults in China and explored potential explanatory factors. Overall, the LTCI was found to improve the health of Chinese older adults. However, it was also associated with widening health inequality among older residents across income classes and between urban and rural areas.

Discovery of Novel Neonicotinoids with Conjugated Diene Enabled by Cascade Allylation/Isomerization Synthetic Methodology.

As one of the most significant insecticides, neonicotinoids have played a pivotal role in crop protection and public sanitation. However, the high resistance and bee toxicity of neonicotinoid insecticides have attracted considerable attention. Herein, a series of neonicotinoid compounds with conjugated diene moieties were synthesized through the cascade allylation/isomerization reaction.

A method for monitoring three dimensional surface deformation in mining areas combining SBAS-InSAR, GNSS and probability integral method.

In the process of mineral resource extraction, monitoring surface deformation is crucial for ensuring the safety of engineering and ground infrastructure. Monitoring complete three-dimensional surface deformation is particularly significant. Traditional synthetic aperture radar (InSAR) technology provides deformation components only along the line of sight (LOS) and often lacks sufficient effective data in vegetation-covered mining areas and mining subsidence centers.

Chromosome-level genome assembly and annotation of Japanese anchovy (Engraulis japonicus).

The Japanese anchovy (Engraulis japonicus), a finfish with the largest biomass of a single species in the Yellow and East China Seas, plays an important pivotal role in converting zooplanktons into high trophic fish in the food web. As a result, the fish is regard as a key species in its habiting ecosystem. However, the lack of genomic resources hampers our understanding of its genetic diversity and differentiation, as well as the evolutionary dynamics.

Dapagliflozin plus calorie restriction for remission of type 2 diabetes: multicentre, double blind, randomised, placebo controlled trial.

Objective: To assess the effect of dapagliflozin plus calorie restriction on remission of type 2 diabetes.

Design: Multicentre, double blind, randomised, placebo controlled trial.

Setting: 16 centres in mainland China from 12 June 2020 to 31 January 2023.

Use of corn carbon as an additive to enhance magnesium metal self-corrosion and recover phosphorus from swine wastewater in the form of struvite.

Recovery of phosphate from swine wastewater is significant for alleviating eutrophication in aquatic ecosystems and addressing the increasing scarcity of phosphorus resources. In this study, a method for phosphate recovery from swine wastewater using corn carbon as an additive and non-dynamic magnesium metal self-corrosion was studied. The effects of reaction time, C:Mg mass ratio, stirring rate, and aeration rate on phosphate recovery were discussed, and eight experimental models were explored.

Mechanistic insights and optimization of lignin depolymerization into aromatic monomers using vanadium-modified Dawson-type polyoxometalates.

Lignin, as the largest renewable aromatic resource, has significant opportunities for producing high-value products via catalytic depolymerization. However, its complex structure and stable chemical bonds present challenges to its transformation. This study explores the catalytic depolymerization of lignin to aromatic monomers by means of Dawson-type phosphomolybdovanadate polyoxometalates (POMs), understanding the underlying mechanisms.

Protective effect of ulinastatin against negative inflammatory response and organ dysfunction in acute aortic dissection surgery: The PANDA trial.

Ulinastatin is a protease-inhibiting drug with anti-inflammatory and other pharmacological properties. Little is known regarding its role following acute type A aortic dissection (ATAAD) surgery. We perform a randomized controlled trial to investigate the protective effect of ulinastatin against negative inflammatory response and organ dysfunction in ATAAD surgery (PANDA).

Transport and action of sesame protein-derived ACE inhibitory peptides ITAPHW and IRPNGL.

Vascular endothelial dysfunction is an important pathogenic factor in hypertension, in which angiotensin-converting enzyme (ACE) plays an important role. Peptides that bind to ACE may attenuate vascular endothelial dysfunction by altering the structure of ACE. This study demonstrated that ITAPHW and IRPNGL were resistant to simulated gastrointestinal fluid and were transported across the Caco-2 monolayer via the intercellular space, with ITAPHW showing a high apparent permeability coefficient of (1.

A conformational switch-controlled RNA sensor based on orthogonal dCas12a for RNA imaging in live cells.

RNA imaging technology is essential for understanding the complex RNA regulatory mechanisms and serves as a powerful tool for disease diagnosis. However, conventional RNA imaging methods often require multiple fluorescent tags for the specific labeling of individual targets, complicating both the imaging process and subsequent analysis. Herein, we develop an RNA sensor that integrates a blocked CRISPR RNA (crRNA)-based conformational switch with a controllable CRISPR activation (CRISPRa) system and apply for RNA imaging.

Triple knockdown of , , and in T cells reduces CAR-T cell toxicity but preserves activity against solid tumors in mice.

Chimeric antigen receptor (CAR)-T cell therapies have revolutionized the landscape of cancer treatment, in particular in the context of hematologic malignancies. However, for solid tumors that lack tumor-specific antigens, CAR-T cells can infiltrate and attack nonmalignant tissues expressing the CAR target antigen, leading to on-target, off-tumor toxicity. Severe on-target, off-tumor toxicities have been observed in clinical trials of CAR-T therapy for solid tumors, highlighting the need to address this issue.

De novo biosynthesis of quercetin in Yarrowia Lipolytica through systematic metabolic engineering for enhanced yield.

Kaempferol and quercetin possess various biological activities, making them valuable in food and medicine. However, their production via traditional methods is often inefficient. This study aims to address this gap by engineering the yeast Yarrowia lipolytica to achieve high yields of these flavonoids.

Comprehensive Chlorine Suppression: Advances in Materials and System Technologies for Direct Seawater Electrolysis.

Seawater electrolysis offers a promising pathway to generate green hydrogen, which is crucial for the net-zero emission targets. Indirect seawater electrolysis is severely limited by high energy demands and system complexity, while the direct seawater electrolysis bypasses pre-treatment, offering a simpler and more cost-effective solution. However, the chlorine evolution reaction and impurities in the seawater lead to severe corrosion and hinder electrolysis's efficiency.

LINC02418 suppresses endometrial cancer progression via regulating miR-494-3p/RASGRF1 axis.

Long non-coding RNAs (lncRNAs) have emerged as pivotal regulatory molecules in cancer biology. Among these, long intergenic non-protein coding RNA 02418 (LINC02418), a recently identified lncRNA, has been linked to endometrial cancer (EC), although its function and operational mechanisms are largely unclear. The present investigation aims to elucidate the molecular mechanism through which LINC02418 influences EC pathogenesis.

Conjugation of PDLA onto MgO microspheres: comparison between solution grafting and melt grafting methods.

Magnesium oxide (MgO) is known for its bioactivity and osteoconductivity when incorporated into biodegradable poly(lactic acid) (PLA), whereas the weak interfacial bonding between MgO microspheres (mMPs) and PLA often leads to suboptimal composite properties with uncontrollable functionality. Conjugation of mMPs with PLA may offer a good way to enhance their compatibility. In this study, we systematically investigated two grafting techniques, solution grafting (Sol) and melt grafting (Mel), to decorate poly (D-lactic acid) (PDLA) on mMPs pre-treated by prioritized hydration to obtain Sol MPs and Mel MPs, in order to optimize the grafting efficiency and improve their controllability in the properties including the crystal structure and surface morphology.

The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew.

Powdery mildew poses a significant threat to global wheat production and most cloned and deployed resistance genes for wheat breeding encode nucleotide-binding and leucine-rich repeat (NLR) immune receptors. Although two genetically linked NLRs function together as an NLR pair have been reported in other species, this phenomenon has been relatively less studied in wheat. Here, we demonstrate that two tightly linked NLR genes, RXL and Pm5e, arranged in a head-to-head orientation, function together as an NLR pair to mediate powdery mildew resistance in wheat.

Efficient Carbon-Based Optoelectronic Synapses for Dynamic Visual Recognition.

The human visual nervous system excels at recognizing and processing external stimuli, essential for various physiological functions. Biomimetic visual systems leverage biological synapse properties to improve memory encoding and perception. Optoelectronic devices mimicking these synapses can enhance wearable electronics, with layered heterojunction materials being ideal materials for optoelectronic synapses due to their tunable properties and biocompatibility.

A two-dimensional fluorescence and chemiluminescence orthogonal probe for discriminating and quantifying similar proteins.

Given that proteins with minor variations in amino acid sequences cause distinct functional outcomes, identifying and quantifying similar proteins is crucial, but remains a long-standing challenge. Herein, we present a two-dimensional orthogonal fluorescence and chemiluminescence design strategy for the probe DCM-SA, which is sequentially activated by albumin-mediated hydrolysis, exhibiting light-up fluorescence and photo-induced cycloaddition generating chemiluminescence, enabling orthogonal signal amplification for discrimination of subtle differences between similar proteins. By orthogonalizing these dual-mode signals, a two-dimensional work curve of fluorescence and chemiluminescence is established to distinguish and quantify similar proteins HSA and BSA.

Environmental Impact of Meat Protein Substitutes: A Mini-Review.

The expansion of alternative food industries, including cultured meat, is often promoted as a strategy to reduce environmental pollution, particularly greenhouse gas emissions. However, comprehensive data on the environmental impacts of these industries remains limited. This study examines the environmental impacts of traditional meat and meat substitute production, highlighting their respective advantages and disadvantages.

Cell-Based Meat Safety and Regulatory Approaches: A Comprehensive Review.

Cell-based meat (CBM) technology is a highly promising alternative to traditional animal agriculture, with considerable advantages in terms of sustainability, animal welfare, and food security. Nonetheless, CBM's successful commercialization is dependent on efficiently dealing with several critical concerns, including ensuring biological, chemical, and nutritional safety as well as navigating the global regulatory framework. To ensure CBM's biological safety, detecting and mitigating any potential hazards introduced during the manufacturing process is crucial.