Application of the AgNOR method to cell imprints of lymphoid tissue.

The argyrophil (AgNOR) staining technique for nucleolar organizer regions was applied to both cell imprint preparations and 3 microns sections of 40 specimens of lymphoid tissue (10 normal tonsil, 10 reactive follicular hyperplasia, and 10 low-grade and 10 high-grade non-Hodgkin's lymphomas). The mean AgNOR count per nucleus was higher for imprint preparations than for 3 microns sections for each group of specimens (P less than 0.01).

Silent ischaemia following myocardial infarction: frequency, characteristics and prognosis.

Pre-discharge exercise tests were performed in 359 survivors of an acute myocardial infarction to determine the frequency, characteristics and prognostic implications of silent ischaemia. Tests were negative in 152 patients (42%), silent ischaemia was observed in 103 (29%) and painful ischaemia in 82 (23%). Heart rates at the development of ischaemia and the final double products were similar in both ischaemic groups but patients with silent ischaemia were able to exercise for longer (13.

Susceptibility to tuberculosis in patients with coeliac disease.

An increased prevalence of past tuberculosis is reported in an adult coeliac population. Of 76 adult coeliac disease patients, 6 had had a history of tuberculosis. This compared with the finding of no cases in a population of 81 patients with non-inflammatory bowel diseases, (p = 0.

Does beta adrenergic blockade influence the prognostic implications of post-myocardial infarction exercise testing?

The influence of beta blockade on the ability of ST depression, during pre-discharge exercise testing, to predict coronary anatomy and subsequent complications was studied in 300 consecutive post-infarct patients, 125 of whom underwent cardiac catheterisation. At the time of exercise 62 patients were taking a beta blocker. The exercise test had a higher sensitivity in predicting multivessel disease in patients who were not taking beta blockers than in patients who were (95% v 76%).

Correlation between NOR sizes and numbers in non-Hodgkin's lymphomas.

A series of 36 cases of non-Hodgkin's lymphomas (NHL) has been studied by means of the argyrophil (AgNOR) method for nuclear organizer regions (NORs). Morphometric analysis of highly magnified photographic images of light microscope preparations of the AgNORs was performed by means of an interactive image analysis system. It was observed that in the low-grade specimens (where NORs are less frequent than in those of high-grade histology), the AgNOR sites were highly significantly (P less than 0.

Salivary gland tumours studied by means of the AgNOR technique.

Difficulty is sometimes encountered in distinguishing between pleomorphic adenoma, adenoid cystic carcinoma and adenocarcinoma, especially in small biopsies from salivary glands. The argyrophil (AgNOR) staining technique for nucleolar organizer regions (NORs) has been applied to a series of benign and malignant salivary gland tumours. We studied 35 salivary gland tumours, 13 benign and 22 malignant.

Nucleolar organising regions in cervical intraepithelial neoplasia.

The variations in the numbers of nucleolar organising regions (NORs) among different grades of cervical intraepithelial neoplasia (CIN) were investigated using a silver staining technique. Twenty four biopsy specimens were studied (six normal and six of each of the three grades of CIN) by staining paraffin wax sections using a silver (AgNOR) method that stains the NORS as multiple black dots within nuclei (AgNORs). The number of AgNORs in the nuclei of cells in the basal half of the squamous epithelium was counted, and the average number of AgNORs in each cell calculated for each specimen (the AgNOR count).

Gastric adenocarcinoma associated with adenomyoma of the stomach.

We report a case of gastric adenomyoma contiguous with adenocarcinoma of the stomach. Although a similar association is well recognized in the duodenum, we believe this to be the first documented case in the stomach. We review the literature on adenomyomata and discuss the implications of this finding.

Effect of gliclazide on thromboxane B2, parameters of haemostasis, fluorescent IgG and lipid peroxides in non-insulin dependent diabetes mellitus.

Abnormalities of platelet aggregation, and increased levels of lipid peroxides and denatured proteins have been implicated in the pathogenesis of diabetic microangiopathy. Seventeen non-insulin dependent diabetics (mean age 65.7) on established sulphonylurea therapy were converted to an equivalent dose of gliclazide.

S-100 protein localization in minor salivary gland tumours: an aid to diagnosis.

The clinician is almost entirely dependent on the histopathologist to accurately diagnose minor salivary gland tumours, but in some cases the histological interpretation of the specimen is very difficult. Recently it has been demonstrated using immunohistochemical techniques that S-100 protein is present in some salivary gland tissues and its localization has been used as an aid in the differentiation of major salivary gland tumours. To assess its value in the diagnosis of minor salivary gland tumours it was localized in sections from 15 such tumours using both a standard peroxidase-antiperoxidase (PAP) and a newly developed immunogold-silver staining sequence (IGSS) technique.

Nucleolar organiser regions in normal, cirrhotic, and carcinomatous livers.

A series of 54 liver biopsy specimens was studied by means of the argyrophil (AgNOR) technique for nucleolar organiser region (NOR)-associated proteins. These included normal livers and livers affected by chronic active hepatitis, cirrhosis, hepatocellular carcinoma and adenoma. Four of the cases of cirrhosis showed liver cell dysplasia.

[Molecular epidemiology of rotaviruses].

Group A rotaviruses have been recognized worldwide as the main viral cause of acute infantile gastroenteritis and of diarrhoea of the young of a wide variety of animal species. The molecular epidemiology of rotaviruses has revealed an extensive genomic and antigenic variability of successively occurring or cocirculating human group A rotaviruses. Group B rotaviruses have been found to cause outbreaks of acute gastroenteritis among adults in China.

Tuberculous stenosis of the trachea.

A patient is described who presented with symptoms suggestive of asthma but was subsequently found to have a stenotic lesion of the lower trachea and main bronchi. Bronchoscopic biopsy of the lesion revealed tuberculosis. There was a good response to anti-tuberculosis chemotherapy, with no residual stenosis.

Cervical Crohn's disease with oesophago-pulmonary fistula.

We report what we believe to be the first case of Crohn's disease involving the cervical oesophagus with an oesophago-pulmonary fistula to the apex of the lung. The proximal location of the disease prevented radical therapy and the patient eventually died from chronic sepsis.

Transfer factor for carbon monoxide in patients with diabetes with and without microangiopathy.

The transfer coefficient (KCO) was significantly lower in diabetic patients with microangiopathy than in a matched group without this complication. This may reflect microangiopathy in the pulmonary circulation.

Which exercise test variables are of prognostic importance post-myocardial infarction?

The prognostic value of parameters noted on pre-discharge exercise testing was assessed in 300 survivors of acute myocardial infarction. Exercise testing was performed at a mean of 9 days post-infarction. Each patient's data were studied for the presence of ST-segment depression or elevation greater than or equal to 0.

Does acute-phase beta-blockade reduce mortality in acute myocardial infarction by limiting infarct size?

The mechanism by which early intervention with beta-blockers reduces mortality in acute myocardial infarction is unclear. Therefore the effects of intravenous, followed by oral, metoprolol on indices of infarct size were studied in a double-blind fashion with a median delay of 6.75 hours from onset of symptoms.

Amoebic liver abscess in a bisexual man.

A bisexual man who had not travelled outside the U.K. had an amoebic liver abscess.

Efficacy of oral acyclovir treatment of acute herpes zoster.

Oral acyclovir, 800 mg five times per day for seven days, was compared with placebo in a randomized, double-blind trial conducted at three centers in the United Kingdom. The study group consisted of 364 elderly immunocompetent patients with herpes zoster who were entered within 72 hours of the onset of rash. Acyclovir significantly reduced the times to last new lesion formation (p less than 0.

Scanning electron microscopy of the upper urinary tract in transitional cell carcinoma of the renal pelvis.

Three nephrectomy specimens with transitional cell carcinoma (TCC) of the renal pelvis were thoroughly examined by both light and scanning electron microscopy. The tumours as well as the urothelium of the upper urinary tract were studied. In all three cases, extensive areas of the urothelium, even in places remote from the tumours, were found by scanning electron microscopy (SEM) to be covered by pleomorphic microvilli.