The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization.

Background: To evaluate the impact of follicle-flushing during oocyte collection on embryo development potential retrospectively.

Methods: A total of 1714 cases, including 133 who experienced retrieval difficulty (repeated follicle-flushing) on the day of oocyte retrieval (difficulty group) and the control 1581 cases (control group), were assessed in this retrospective study. The number of oocytes recovered, two pro-nuclei fertilization (2PN-fertilization), day 3 good-quality embryo and day 5/6 blastocyst utilization rates were compared between the difficulty group and control group correspondingly.

Effect of embryo and blastocyst transfer on the birthweight of live-born singletons from FET cycles.

Purpose: To evaluate the effect of culture duration (embryo (day 3) transfer vs. blastocyst (day 5-6) transfer) on the birthweight of singletons from frozen embryo transfer (FET) cycles.

Methods: A total of 1092 singletons were analyzed in this retrospective study.

The clinical application of single-sperm-based SNP haplotyping for PGD of osteogenesis imperfecta.

Osteogenesis imperfecta (OI) is a genetically heterogeneous disorder, presenting either autosomal dominant, autosomal recessive or X-linked inheritance patterns. The majority of OI cases are autosomal dominant and are caused by heterozygous mutations in either the COL1A1 or COL1A2 gene. In these dominant disorders, allele dropout (ADO) can lead to misdiagnosis in preimplantation genetic diagnosis (PGD).