Adriamycin dose and time effects on cell cycle, cell death, and reactive oxygen species generation in leukaemia cells.

We investigate the relative importance of the different mechanisms of Adriamycin, an anthracycline, and their interrelations, in particular the link between cell cycle arrest, cell death, and generation of reactive oxygen species (ROS) that is suspected to be the origin of cardiotoxic side-effects. We introduced a lifetime fluorescence based technology and used videomicrofluorometry, two efficient analytical methods. We show that depending on the doses and time after incubation, ADR will not reach the same compartments (nucleus, mitochondria, cytosol) in the cells, having consequences on the production of ROS, growth arrest pathways and cell death pathways.

Stimulated Raman scattering microscopy by spectral focusing and fiber-generated soliton as Stokes pulse.

We demonstrate stimulated Raman microscopy with broadband pump and Stokes pulses, using spectral focusing to attain spectral resolution and to rapidly acquire spectra within a spectral window determined by the bandwidth of the pulses. As the Stokes pulse, we use the redshifted soliton generated in a photonic crystal fiber, which allows for simple shifting of the accessible spectral window.

Optical properties of dielectric thin films including quantum dots.

Depending on the minimum size of their micro/nanostructure, thin films can exhibit very different behaviors and optical properties. From optical waveguides down to artificial anisotropy, through diffractive optics and photonic crystals, the application changes when decreasing the minimum feature size. Rigorous electromagnetic theory can be used to model most of the components, but, when the size is a few nanometers, quantum theory also has to be used.

Transform-limited spectral compression by self-phase modulation of amplitude-shaped pulses with negative chirp.

Spectral compression by self-phase modulation of amplitude- and phase-shaped pulses is demonstrated as superior compared to pulses that have only been phase shaped. We synthesize linearly negatively chirped parabolic pulses, which we send through a nonlinear photonic crystal fiber, in which self-phase modulation compresses the spectrum of the pulses to within 20% of the Fourier transform limit.

Membrane characterization by microscopic and scattering methods: multiscale structure.

Several microscopic and scattering techniques at different observation scales (from atomic to macroscopic) were used to characterize both surface and bulk properties of four new flat-sheet polyethersulfone (PES) membranes (10, 30, 100 and 300 kDa) and new 100 kDa hollow fibers (PVDF). Scanning Electron Microscopy (SEM) with "in lens" detection was used to obtain information on the pore sizes of the skin layers at the atomic scale. White Light Interferometry (WLI) and Atomic Force Microscopy (AFM) using different scales (for WLI: windows: 900 × 900 µm2 and 360 × 360 µm2; number of points: 1024; for AFM: windows: 50 × 50 µm2 and 5 × 5 µm2; number of points: 512) showed that the membrane roughness increases markedly with the observation scale and that there is a continuity between the different scan sizes for the determination of the RMS roughness.

Whispering gallery modes and other cavity modes for perfect backscattering and blazing.

We demonstrate the possibility to obtain perfect blazing both in Littrow and off-Littrow mountings using diffractive systems consisting of a plane metallic substrate and dielectric structures that can support cavity modes. The resonances are located at a relatively large distance between the metal and the dielectric structure, a condition that prevents the resonance increase of absorption. The high efficiency can be obtained in transverse electric or transverse magnetic polarization and at high incident angles.

Linearly damped modes at gap edges of photonic crystals.

It is shown that for one-dimensional dielectric photonic crystals, the Bloch modes, a vital tool in the analysis of these structures, cannot provide a complete representation of the electromagnetic field at the edges of bandgaps. On these points, the couple of Bloch modes representing the propagation on both sides of the crystal reduces to a single one, with a stationary field, and a complete representation of the field inside the crystal illuminated by a plane wave must include a linearly damped mode (LDM), the amplitude of which behaves linearly in space. The theory of transfer matrices and the use of basic properties of the field allow a precise description of the LDM from a few parameters.

An enhanced contrast to detect bulk objects under arbitrary rough surfaces.

We study a selective light scattering elimination procedure in the case of highly scattering rough surfaces. Contrary to the case of low scattering levels, the elimination parameters are shown to depend on the sample microstructure and to present rapid variations with the scattering angle. On the other hand, when the slope of the surface is moderated, we show that this parameters present smoother variations and little dependence to the microstructure, even when the roughness is high.

Nature and anisotropy of cortical forces orienting Drosophila tissue morphogenesis.

The morphogenesis of developing embryos and organs relies on the ability of cells to remodel their contacts with neighbouring cells. Using quantitative modelling and laser nano-dissection, we probed the mechanics of a morphogenetic process, the elongation of Drosophila melanogaster embryos, which results from polarized cell neighbour exchanges. We show that anisotropy of cortical tension at apical cell junctions is sufficient to drive tissue elongation.

Multiscale analysis of the laser-induced damage threshold in optical coatings.

We have investigated the influence of laser beam size on laser-induced damage threshold (LIDT) in the case of single- and multiple-shot irradiation. The study was performed on hafnia thin films deposited with various technologies (evaporation, sputtering, with or without ion assistance). LIDT measurements were carried out at 1064 nm and 12 ns with a spot size ranging from a few tens to a few hundreds of micrometers, in 1-on-1 and R-on-1 modes.

Spectral analysis of three-dimensional photonic jets.

The spatial and spectral properties of three-dimensional photonic jets are studied in a framework employing rigorous Lorentz-Mie theory. The contributions to the field from each spectral component are studied quantitatively and highlight the distinctive features of photonic jets. In particular, the role of evanescent field in photonic jets generated by small spheres is investigated.

Why a harmonic solution for lossless, perfectly homogeneous, left-handed material cannot exist.

In a preceding paper [J. Opt. Soc.

Mean-square coherent light.

Mean-square coherent light is defined as light that is able to interfere with fringes of unit visibility when its electromagnetic field is multiplied by appropriate nonsingular deterministic Jones matrices. It includes light that satisfies the factorization condition at order one and partially polarized light that leads to interference fringes of unit visibility. A necessary and sufficient experimentally measurable condition to determine if two electromagnetic fields are mean-square coherent is established.

Multiple holographic optical tweezers parallel calibration with optical potential well characterization.

We report the extension to a multi-axes exploration of the potential well reconstruction method against drag force to simultaneously characterize the potential wells of several trapping sites generated with holographic optical tweezers. The final result is a robust, fast and automatic procedure we use to characterize holographic tweezers. We mainly focus on the reliability of the method and its application to address the dependence of the diffraction efficiency with the trap position in a given holographic traps pattern.

Direct imaging of photonic nanojets.

We report the direct experimental observation of photonic nanojets created by single latex microspheres illuminated by a plane wave at a wavelength of 520 nm. Measurements are performed with a fast scanning confocal microscope in detection mode, where the detection pinhole defines a diffraction-limited observation volume that is scanned in three dimensions over the microsphere vicinity. From the collected stack of images, we reconstruct the full 3 dimensional photonic nanojet beam.

Total absorption of unpolarized light by crossed gratings.

We present both experimental and numerical data showing the absorption of unpolarized, normally incident light by a gold crossed grating having a shallow sinusoidal profile. We show furthermore that the total absorption of unpolarized light can be achieved for an angle of incidence of 30 degrees with a crossed grating having its period adjusted appropriately from the normal incidence case to preserve the plasmonic resonance responsible for the enhanced absorptance. We contrast the process for achieving high absorptance in the principal plane of incidence aligned with the grooves of one of the gratings, with that for the principal plane at 45 degrees to each grating.

Laser damage resistance of hafnia thin films deposited by electron beam deposition, reactive low voltage ion plating, and dual ion beam sputtering.

A comparative study is made of the laser damage resistance of hafnia coatings deposited on fused silica substrates with different technologies: electron beam deposition (from Hf or HfO(2) starting material), reactive low voltage ion plating, and dual ion beam sputtering. The laser damage thresholds of these coatings are determined at 1064 and 355 nm using a nanosecond pulsed YAG laser and a one-on-one test procedure. The results are associated with a complete characterization of the samples: refractive index n measured by spectrophotometry, extinction coefficient k measured by photothermal deflection, and roughness measured by atomic force microscopy.

Determination of the refractive indices of layers in a multilayer stack by a guided-wave technique.

The m-lines technique is used to measure the refractive indices and thicknesses of layers embedded in a multilayer stack. The multilayer considered is deposited by ion plating. Its formula is silica-H-L-H-L-H-air, where H and L denote Ta(2)O(5) and SiO(2)lambda/4 layers, respectively, with lambda = 514.

Light emission from sources located within metallodielectric planar microcavities.

A simple, rigorous electromagnetic formula is derived for predicting the electromagnetic power provided by sources located in transparent or dissipative planar microcavities. With this simple approach, we compare numerically and experimentally the electromagnetic power that escapes the microcavity when the source is located in a metallodielectric or in an all-dielectric resonant planar structure. Although a strong light-extraction coefficient might be expected for metallodielectric microcavities, we show that these attractive structures suffer from metal absorption even when thin metallic layers are used.

Design of a full-silica pulse-compression grating.

A diffraction grating engraved on a two-dimensional photonic crystal composed of square air holes in a silica matrix is numerically studied for the compression of ultrashort pulses. The silica is therefore the only solid material of the grating, and the reflection of the incident beam is based on the contrast of the air and silica refractive indices. This optical component enables the single use of silica as a solid material, presenting a high laser-induced damage threshold.

Minimum description length synthetic aperture radar image segmentation.

We present a new minimum description length (MDL) approach based on a deformable partition--a polygonal grid--for automatic segmentation of a speckled image composed of several homogeneous regions. The image segmentation thus consists in the estimation of the polygonal grid, or, more precisely, its number of regions, its number of nodes and the location of its nodes. These estimations are performed by minimizing a unique MDL criterion which takes into account the probabilistic properties of speckle fluctuations and a measure of the stochastic complexity of the polygonal grid.

Toward the simulation of the strain of female pelvic organs.

Surgical simulators have undergone a significant development, especially since the rise of mini-invasive surgery. The main simulators of digestive surgery have been developed for solid organs such as the liver and spleen. Studies relating to soft tissues like the pelvic organs are rare.

Non-Bloch plasmonic stop-band in real-metal gratings.

Recent studies of plasmon surface wave (PSW) propagation in short-period perfectly conducting gratings have shown formation of stop-band that are not linked to the interaction between two (counter) propagating surface waves. We study the properties of this stop-band in real metals. While for both perfectly conducting and real metals the propagation constant of PSW grows with the groove height, the stop-band in real metals appears for groove heights significantly smaller than in perfect metals.

Nonparametric statistical snake based on the minimum stochastic complexity.

We propose a nonparametric statistical snake technique that is based on the minimization of the stochastic complexity (minimum description length principle). The probability distributions of the gray levels in the different regions of the image are described with step functions with parameters that are estimated. The segmentation is thus obtained by minimizing a criterion that does not include any parameter to be tuned by the user.

Ellipsometry of reflected and scattered fields for the analysis of substrate optical quality.

Specular ellipsometry is a well-known and efficient technique to characterize surfaces and coatings. This technique has been extended to the measurement of scattered light. We present an experimental setup, using a polarization modulator, which permits us to characterize transition layers and roughness without a calibration procedure.