Cadaverine Production by Using Cross-Linked Enzyme Aggregate of Lysine Decarboxylase.

Lysine decarboxylase (CadA) converts -lysine into cadaverine (1,5-pentanediamine), which is an important platform chemical with many industrial applications. Although there have been many efforts to produce cadaverine through the soluble CadA enzyme or whole cells overexpressing the CadA enzyme, there have been few reports concerning the immobilization of the CadA enzyme. Here, we have prepared a cross-linked enzyme aggregate (CLEA) of CadA and performed bioconversion using CadA.

Characterization of Proteus vulgaris K80 lipase immobilized on amine-terminated magnetic microparticles.

Proteus vulgaris K80 lipase was expressed in Escherichia coli BL21 (DE3) cells and immobilized on amine-terminated magnetic microparticles (Mag-MPs). The immobilization yield and activity retention were 84.15% and 7.