2 results match your criteria: "Diabetes Research Centre of Guangdong Province[Affiliation]"
[The functional analysis of glucokinase gene E339K mutation].
Zhonghua Nei Ke Za Zhi
July 2010
Department of Endocrinology, the Third Affiliated Hospital, Sun Yat-sen University, Diabetes Research Centre of Guangdong Province, Guangzhou 510630, China.
Objective: To explore the molecular mechanisms of glucokinase (GCK) E339K mutation resulting in maturity-onset diabetes of the young-2 (MODY2).
Methods: Fasting plasma glucose (FPG), oral glucose tolerance test (OGTT) overload 2 h glucose (2hPG), glycosylated hemoglobin A1c (HbA1c) and fasting insulin (FIns) level were measured, respectively. Mutant glutathione S-transferase (GST)-GCK-cDNA was constructed with site-directed mutagenesis.
[Identification of a novel glucokinase-E339K mutation in a Chinese maturity onset diabetes of the young 2 pedigree].
Zhonghua Nei Ke Za Zhi
September 2009
Department of Endocrinology, The Third Affiliated Hospital, Sun Yat-sen University, Diabetes Research Centre of Guangdong Province, Guangzhou 510630, China.
Objective: To identify the related gene for a typical maturity onset diabetes of the young 2 (MODY2) pedigree.
Methods: The genomic DNA of all the members of the pedigree was extracted and then PCR amplification on 5', 3' untranslated region and exon 1 - 10 of glucokinase (GCK) gene was carried out. Sequencing in both directions was performed to identify mutation on GCK gene.