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Recently unearthed Tinhui Medical Slips, dating back to 2nd century BCE, demonstrates the 12th meridian in Chinese medicine as an anatomy-based discovery. This is equivalent to the persistent median artery, which was later incorporated into Neijing meridian theory and set the foundation for traditional acupuncture today.

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Persistent median artery (PMA) has been recognized as a major arterial variant of the forearm in about 30% adults and it also shows a continuously increasing trend in the last two hundred years. This may explain well the mystery why a "new meridian" was added to the older 11-meridian system over two thousand years ago in Chinese medicine. The 12th meridian, Hand Jue Yin Meridian, happens to run along the same pathway as PMA.

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