2 results match your criteria: "Departments of Molecular Biosciences and Oncology University of Texas at Austin Austin[Affiliation]"
Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers.
PLoS Genet
September 2024
Departments of Molecular Biosciences and Oncology University of Texas at Austin Austin, Texas, United States of America.
Article Synopsis
- Human plasma contains short structured RNAs called FLEXI RNAs, which have potential for use as biomarkers; a study identified over 9,000 different FLEXIs in human cell lines, highlighting their specific expression patterns depending on the cell type.
- Researchers found 126 RNA-binding proteins (RBPs) that interact with FLEXI RNAs, which include factors involved in splicing, transcription, and cell growth, showing that these proteins play a significant role in regulating the RNA's functions.
- The study also revealed that certain groups of RBPs tend to bind to FLEXIs from functionally related genes, indicating that these RBPs may work together to coordinate the expression of genes
Reverse transcriptase-Cas1 (RT-Cas1) fusion proteins found in some CRISPR systems enable spacer acquisition from both RNA and DNA, but the mechanism of RNA spacer acquisition has remained unclear. Here, we found RT-Cas1/Cas2 adds short 3'-DNA (dN) tails to RNA protospacers enabling their direct integration into CRISPR arrays as 3'-dN-RNA/cDNA duplexes or 3'-dN-RNAs at rates comparable to similarly configured DNAs. Reverse transcription of RNA protospacers occurs by multiple mechanisms, including recently described initiation, protein priming with any dNTP, and use of short exogenous or synthesized DNA oligomer primers, enabling synthesis of cDNAs from diverse RNAs without fixed sequence requirements.
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