Localization of Fructan Metabolism in the Vacuoles Isolated from Protoplasts of Jerusalem Artichoke Tubers (Helianthus tuberosus L.).

Protoplasts were prepared from tubers of Helianthus tuberosus in the developing and in the resting stage of development. Vacuoles were isolated from the protoplasts and purified by sedimentation through a density gradient of glycine betaine. All the fructan (inulin) with a DP ⩾ 3 (i.