1 results match your criteria: "Departments of Biochemistry and Molecular Biology and Michigan State University[Affiliation]"
Structural determinants allowing transferase activity in SENSITIVE TO FREEZING 2, classified as a family I glycosyl hydrolase.
J Biol Chem
September 2014
Departments of Biochemistry and Molecular Biology and Michigan State University, East Lansing, Michigan 48824.
Article Synopsis
- SENSITIVE TO FREEZING 2 (SFR2) is a family I glycosyl hydrolase that has shown galactosyltransferase activity in the model plant Arabidopsis thaliana, which could be leveraged for biocatalysis.
- Research is focused on how SFR2 evolved to function as a transferase, involving methods like activity assays and molecular modeling to explore its structural and functional relationships.
- Key findings reveal that, despite its strong conservation with glycosyl hydrolases, SFR2 lacks hydrolase activity and has three specific structural regions (a loop insertion, C-terminal peptide, and adjacent hydrophobic patch) that are essential for its transferase function.