Immune responses of cattle vaccinated by various routes with Mycobacterium bovis Bacillus Calmette-Guérin (BCG).

Background: Mycobacterium bovis BCG is the human tuberculosis vaccine and is the oldest vaccine still in use today with over 4 billion people vaccinated since 1921. The BCG vaccine has also been investigated experimentally in cattle and wildlife by various routes including oral and parenteral. Thus far, oral vaccination studies of cattle have involved liquid BCG or liquid BCG incorporated into a lipid matrix.

Chronic and infectious respiratory mortality and short-term exposures to four types of pollen taxa in older adults in Michigan, 2006-2017.

Introduction: Levels of plant-based aeroallergens are rising as growing seasons lengthen and intensify with anthropogenic climate change. Increased exposure to pollens could increase risk for mortality from respiratory causes, particularly among older adults. We determined short-term, lag associations of four species classes of pollen (ragweed, deciduous trees, grass pollen and evergreen trees) with respiratory mortality (all cause, chronic and infectious related) in Michigan, USA.

The expansion and loss of specific olfactory genes in relatives of parasitic lice, the stored-product psocids (Psocodea: Liposcelididae).

Background: Booklice, belonging to the genus Liposcelis (Psocodea: Liposcelididae), commonly known as psocids, infest a wide range of stored products and are implicated in the transmission of harmful microorganisms such as fungi and bacteria. The olfactory system is critical for insect feeding and reproduction. Elucidating the molecular mechanisms of the olfactory system in booklice is crucial for developing effective control strategies.

Cannabidiol abrogates cue-induced anxiety associated with normalization of mitochondria-specific transcripts and linoleic acid in the nucleus accumbens shell.

Anxiety disorders are one of the top contributors to psychiatric burden worldwide. Recent years have seen a dramatic rise in the potential anxiolytic properties ascribed to cannabidiol (CBD), a non-intoxicating constituent of the Cannabis Sativa plant. This has led to several clinical trials underway to examine the therapeutic potential of CBD for anxiety disorders.

Tests for segregation distortion in tetraploid F1 populations.

In tetraploid F1 populations, traditional segregation distortion tests often inaccurately flag SNPs due to ignoring polyploid meiosis processes and genotype uncertainty. We develop tests that account for these factors. Genotype data from tetraploid F1 populations are often collected in breeding programs for mapping and genomic selection purposes.

Molecular regulation and domestication of parthenocarpy in cucumber.

Parthenocarpy is a pivotal trait that enhances the yield and quality of fruit crops by enabling the development of seedless fruits. Here we unveil a molecular framework for the regulation and domestication of parthenocarpy in cucumber (Cucumis sativus L.).

Reproductive and persistence strategy of the liverwort Arnellia fennica after the last glaciation in the area of disjunction in Central Europe (Polish Tatra Mountains, carpathians).

The liverwort Arnellia fennica has a circumarctic distribution with disjunct and scarce localities in the Alps, Carpathians, and Pyrenees. Within the Carpathians, it is only known from the Tatra Mountains (in Poland), where so far only four occurrences have been documented in the forest belt of the limestone part of the Western Tatras. The species is considered a tertiary relict, which owes its survival during the last glaciation period to low-lying locations in areas not covered by ice.

Elemental cryo-imaging reveals SOS1-dependent vacuolar sodium accumulation.

Increasing soil salinity causes significant crop losses globally; therefore, understanding plant responses to salt (sodium) stress is of high importance. Plants avoid sodium toxicity through subcellular compartmentation by intricate processes involving a high level of elemental interdependence. Current technologies to visualize sodium, in particular, together with other elements, are either indirect or lack in resolution.

Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies.

The remarkable diversity of insect pigmentation offers a captivating avenue for studying evolution and genetics. In tephritids, understanding the molecular basis of mutant traits is also crucial for applied entomology, enabling the creation of genetic sexing strains through genome editing, thus facilitating sex-sorting before sterile insect releases. Here, we present evidence from classical and modern genetics showing that the black pupae (bp) phenotype in the GUA10 strain of Anastrepha ludens is caused by a large deletion at the ebony locus, removing the gene's entire coding region.

Draft genome sequence of Kei apple, an underutilized African tree crop.

To address food and nutrition security in the face of burgeoning global populations and erratic climatic conditions there is a need to include nutrient dense, climatic resilient but neglected indigenous fruit trees in agrifood systems. Here we present the draft genome sequence of Kei Apple, Dovyalis afra, a neglected indigenous African fruit tree with untapped potential to contribute to nutrient security and improved livelihoods. Our long-read-based genome assembly comprises 440 Mbp sequence across 1190 contigs with a N50 and L50 of 13.

Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer's activities.

Nanotechnology has experienced significant advancements, attracting considerable attention in various biomedical applications. This innovative study synthesizes and characterizes Ge/PLA/AuNCs (gelatin/PLA/gold nanocomposites) using Syzygium cumini extract to evaluate their various biomedical applications. The UV-Visible spectroscopy results in an absorption peak at 534 nm were primarily confirmed by Ge/PLA/AuNCs synthesis.

A Chromosome level assembly of pomegranate (Punica granatum L.) variety grown in arid environment.

The pomegranate (Punica granatum L.) is an ancient fruit-bearing tree known for its nutritional and antioxidant properties. They originated from the Middle East in regions having large farms including mountainous regions of Al-Baha in Saudi Arabia.

The underappreciated roles of aboveground vertebrates on belowground communities.

In recent decades, evidence of interactions between aboveground and belowground (i.e., soil) subsystems has accumulated.

Antibody functionalized curcuma-derived extracellular vesicles loaded with doxorubicin overcome therapy-induced senescence and enhance chemotherapy.

Conventional cancer treatments often induce a sustained DNA damage response (DDR) in tumor cells, leading to therapy-induced senescence (TIS), characterized by permanent cell cycle arrest and resistance to apoptosis. These senescent cells secrete senescence-associated secretory phenotypes (SASP), which can promote tumor progression and create an immunosuppressive microenvironment. This study introduces a novel approach to enhance chemotherapy efficacy by using functionalized curcuma-derived extracellular vesicles (DR5-CNV/DOX) to target and eliminate senescent tumor cells and inhibit their SASP.

Antimicrobial potential of the leaves of Citrus grandis (L.) Osbeck collected from Iraq: Bioassay-guided isolation of sinensetin as the anti-MRSA compound.

Infections caused by antibiotic-drug-resistant microorganisms are a major global health concern, and they result in millions of deaths every year. Methicillin-resistant Staphylococcus aureus (MRSA) is one of such drug-resistant microbial strains, and new and effective antimicrobial agents are desperately needed to combat infections caused by MRSA. In the search for effective anti-MRSA agents, the leaves of Citrus grandis (Rutaceae), also known as C.

Recent advances in the synthesis and application of biomolecular condensates.

Biomolecular condensates (BMCs) represent a group of organized and programmed systems that participate in gene transcription, chromosome organization, cell division, tumorigenesis, and aging. However, the understanding of BMCs in terms of internal organizations and external regulations remains at an early stage. Recently, novel approaches such as synthetic biology have been used for de novo synthesis of BMCs.

Mechanistic insights into ferroptosis and apoptosis pathways: Synergistic effects of multi-organ toxicity and transgenerational effects induced by co-exposure of epoxiconazole and aflatoxin B1 in zebrafish.

Introduction: In the environment, mycotoxins and fungicides frequently coexist, potentially causing synergistic risks to organisms. Epoxiconazole (EPO) and aflatoxin B1 (AFB1) are a common fungicide and mycotoxins, respectively, which are widely present in the environment and have toxic effects on multiple organs once entering the organism, but it is still unclear whether the co-exposure has a synergistic toxic effect.

Objectives: This study delves into the molecular mechanisms underlying the co-exposure to EPO and AFB1, emphasizing multi-organ toxicity in female zebrafish (F0 generation) and potential transgenerational impacts on the offspring embryos (F1 generation) through multi-omics approaches.

Nitrogen doping turns carbonaceous materials into fast-reacting catalysts for reductive dechlorination.

Nitrogen (N) doping of biomass prior pyrolysis has been identified as an effective approach for enhancing biochar catalytic reactivity. However, high-temperature pyrolysis of N-rich biomass may produce N-devoid biochars with high reactivity, calling for attention to the true causes of the reactivity increases and the role of nitrogen. In this study, N-doped wheat straw biochar (N-BC) materials were produced using urea as N dopant and different pyrolysis conditions, and their catalytic reactivity assessed for the reduction of trichloroethylene (TCE) by green rust (GR), a layered Fe(II)Fe(III) hydroxide.

Large-sized trees and altitude drive aboveground carbon stock in Brazilian Atlantic Cloud Forests: An approach based on carbon hyperdominant taxa.

Research about patterns of aboveground carbon stock (AGC) across different tropical forest types is central to climate change mitigation efforts. However, the aboveground carbon stock (AGC) quantification for Brazilian cloud forest ecosystems along the altitudinal gradient is still scarce. We aimed to evaluate the effects of abiotic and biotic on AGC and the AGC distribution between species and families of tree communities along an altitudinal Brazilian Atlantic cloud forest gradient of the Mantiqueira Mountain Range, Southeastern Brazil.

Polyphenolic contents, antioxidant, and antimicrobial activities of Saccocalyx satureioides Coss. & Dur. essential oil and methanol extracts.

The whole plant Saccocalyx satureioides, an endemic medicinal plant in Algeria, was evaluated for its polyphenolic contents, antioxidant and antimicrobial activities. The polyphenolic contents of the plant methanolic extracts ranged from 170.47 to 285.

Soil microbiome bacteria protect plants against filamentous fungal infections via intercellular contacts.

Bacterial-fungal interaction (BFI) has significant implications for the health of host plants. While the diffusible antibiotic metabolite-mediated competition in BFI has been extensively characterized, the impact of intercellular contact remains largely elusive. Here, we demonstrate that the intercellular contact is a prevalent mode of interaction between beneficial soil bacteria and pathogenic filamentous fungi.

Exploring the efficacy of drought tolerant, IAA-producing plant growth-promoting rhizobacteria for sustainable agriculture.

The growing human population and abiotic stresses pose significant threats to food security, with PGPR favorable as biofertilizers for plant growth and stress relief. In one study, soil samples from both cultivated and uncultivated plants in various cities were used to isolate rhizobacterial populations. Using 50 soil samples from both cultivated and uncultivated plants, isolated rhizobacterial populations were screened for various biochemical changes, PGP activities and morphological characteristics.

Revolutionizing radiotherapy: gold nanoparticles with polyphenol coating as novel enhancers in breast cancer cells-an in vitro study.

Breast cancer is the most common cancer among women, with over 1 million new cases and around 400,000 deaths annually worldwide. This makes it a significant and costly global health challenge. Standard treatments like chemotherapy and radiotherapy, often used after mastectomy, show varying effectiveness based on the cancer subtype.

Essential oil and phenolic compounds in different organs and developmental stages of Monarda didyma L., and their biological activity.

Plant development has a greater impact on the chemical composition of inflorescences than of the leaves and stems of Monarda didyma plants. Monarda didyma L. is a well-known ornamental and aromatic plant.