Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress.

Understanding the molecular mechanisms that confer cold resistance in mammalian cells might be relevant for advancing medical applications. This study aimed to exploit the protective function of Late Embryogenesis Abundant (LEA) proteins, known to provide resistance to low temperatures in extremophiles and plants, by their exogenous expression in mammalian cells, and compare their effects with the well characterized antioxidant, vitamin E.Remarkably, the expression of LEA proteins in mammalian cells exerted cold-protective effect similar to Vitamin E.

Impact of pollution on microbiological dynamics in the pistil stigmas of Orobanche lutea flowers (Orobanchaceae).

Our understanding of the basic relationships of microbiota associated with flowers is still quite limited, especially regarding parasitic plant species. The transient nature of flower parts such as pistil stigmas provides a unique opportunity for temporal investigations. This is the first report of the analysis of bacterial and fungal communities associated with the pistil stigmas of the lucerne parasite, Orobanche lutea.

Host Plant Odour and Sex Pheromone are Integral to Mate Finding in Codling Moth.

The great diversity of specialist plant-feeding insects suggests that host plant shifts may initiate speciation, even without geographic barriers. Pheromones and kairomones mediate sexual communication and host choice, and the response to these behaviour-modifying chemicals is under sexual and natural selection, respectively. The concept that the interaction of mate signals and habitat cues facilitates reproductive isolation and ecological speciation is well established, while the traits and the underlying sensory mechanisms remain unknown.

Complete genome sequence of , the causal agent of goldthread anthracnose disease.

The complete genome sequence of , a goldthread anthracnose pathogen, was sequenced using PacBio Revio and MGI DNBSEQ-T7 PE150. It contains 10 chromosomes, 5 mini chromosomes, a circular mitochondrial chromosome, and 13,129 genes predicted with RNA-Seq data in a 52.13-Mb genome with an of 5.

Citrus Black Spot Symptoms on Fruit Exposed to Infections at Different Developmental Stages.

Citrus black spot (CBS), caused by , is an important fungal disease of citrus. Higher CBS severity has been associated with infections at the young and green stages of fruit. The length of the fruit susceptibility period may be influenced by the amount of inoculum and the climate of the citrus growing region.

Morphometric parameters of seeds as a practical method for identifying rare species of the genus L. (Liliaceae) from East Kazakhstan region.

The genus includes some of the most important ornamental plants. The aim of this work was to study the seed morphology of species from East Kazakhstan, including seed coat structure. An analysis focused on five taxa from various natural environmental conditions.

ubiquitin ligase regulates dichotomous spermatogenesis in .

Spermatogenesis in Lepidoptera holds significant importance due to its unique process of dichotomous spermatogenesis, yielding eupyrene and apyrene spermatozoa through a complex molecular mechanism. While E3 ubiquitin ligases are known to play vital roles in spermatogenesis across various processes, their functions in dichotomous spermatogenesis remain less known. We utilized the RNAi, biochemical and microscopic procedures to unravel the function of in dichotomous spermatogenesis of adult .

An Investigation into the Synthesis and Characterization of Novel Tetrazole Derivatives for Application as Mild Steel Corrosion Inhibitors in a Solution of Hydrochloric Acid.

The objective of the study was to synthesize tetrazole molecules featuring nitro groups positioned at the para and meta locations. We aimed to assess their effectiveness in inhibiting corrosion of mild steel in a 1 M HCl solution at 298 K. Tetrazoles with 2,5-disubstitution were created using [3 + 2] cycloaddition and N-alkylation techniques, with a particular emphasis on synthesizing molecules that contain nitro groups.

Novel D-Ribofuranosyl Tetrazoles: Synthesis, Characterization, In Vitro Antimicrobial Activity, and Computational Studies.

The goal of this study was to synthesize and evaluate new antimicrobial compounds. We specifically focused on the development of 2,5-disubstituted tetrazole derivatives containing the O-methyl-2,3-O-isopropylidene-(D)-ribofuranoside groups through N-alkylation reactions. The synthesized compounds were characterized using H and C nuclear magnetic resonance (NMR) spectroscopy.

Evaluating the antioxidant, anti-inflammatory, and neuroprotective potential of fruiting body and mycelium extracts from edible yellow morel (Morchella esculenta L. Pers.).

Scope: This study aimed to assess the antioxidant, anti-inflammatory, and acetylcholinesterase activities of fruiting bodies (FB) and mycelium (M) extracts of Morchella esculenta L. collected from various regions of Pakistan. The samples included Skardu fruiting body (SKFB) and mycelia Skardu (SKM), Malam Jaba fruiting body (MJFB) and Malam Jaba mycelia (MJM), Krair Mansehra fruiting body (KMFB) and Krair Mansehra mycelia (KMM), and Thandiani fruiting body (TFB) and Thandiani mycelia (TM).

Risk Assessment of Cyclaniliprole Resistance in Peach-Potato Aphid Myzus persicae: Laboratory Selection, Inheritance, and Cross-Resistance Patterns.

Cyclaniliprole, a type of the third-generation anthranilic diamide insecticide, was mainly used for management of various pests. Myzus persicae (Hemiptera: Aphididae), known as the peach-potato aphid, is an economically essential pest with worldwide distribution. However, the risk assessment of cyclaniliprole in M.

Effects of different developmental stages of the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae) on argan (Argania spinosa (L.) Skeels) edible oil and methanolic extracts, and development of eco-friendly control methods based on plant callus oils.

Argan (Argania spinosa (L.) Skeels) is an endangered agroforestry species known for producing one of most expensive and sought-after oils in the world. Argan forests are a suitable habitat for medfly (Ceratitis capitata).

Mucilage-based composites films and coatings for food packaging application: A review.

Developing sustainable and eco-friendly packaging solutions has garnered significant interest in recent years. Mucilage-based coatings and composites offer a promising approach due to their biodegradability, renewable nature, and ability to enhance food quality protection. This review paper discusses the impact of mucilage-based composites and coatings on various packaging applications, focusing on their physical, mechanical, morphological, barrier, and functional properties.

Bacterial and fungal diversity and species interactions inversely affect ecosystem functions under drought in a semi-arid grassland.

Extreme climatic events, such as drought, can significantly alter belowground microbial diversity and species interactions, leading to unknown consequences for ecosystem functioning. Here, we simulated a drought gradient by removing 30 %, 50 %, and 70 % of precipitation in a semi-arid grassland over five years. We assessed the effects of drought on bacterial and fungal diversity, as well as on their species interactions.

RNAi-based biocontrol for crops: a revised expectation for a non-recent technology.

The exogenous application of RNAi technology offers new promises for crops improvement. Cell-based or synthetically produced strands are economical, non-transgenic and could induce the same responses. The substantial population growth demands novel strategies to produce crops without further damaging the environment.

Plant Colonization by Biocontrol Bacteria and Improved Plant Health: A Review.

The use of biological control agents is one of the best strategies available to combat the plant diseases in an ecofriendly manner. Biocontrol bacteria capable of providing beneficial effect in crop plant growth and health, have been developed for several decades. It highlights the need for a deeper understanding of the colonization mechanisms employed by biocontrol bacteria to enhance their efficacy in plant pathogen control.

Effect of Fusion to the LTB Carrier Protein on Coronavirus Spike Protein Vaccine Candidates Produced in Maize.

Coronaviruses continue to disrupt health and economic productivity worldwide. Porcine epidemic diarrhea virus (PEDV) is a devastating swine disease and SARS-CoV-2 is the latest coronavirus to infect the human population. Both viruses display a similar spike protein on the surface that is a target of vaccine development.

Exploring the Potential of Selenium-Containing Amine (Se-AMA) to Enhance Photosynthesis and Leaf Water Content: New Avenues for Carbonic Anhydrase Modulation in .

Global changes and growing demands have led to the development of new molecular approaches to improve crop physiological performances. Carbonic anhydrase (CA) enzymes, ubiquitous across various life kingdoms, stand out for their critical roles in plant photosynthesis and water relations. We hypothesize that the modulators of human CAs could affect plant physiology.

A Comparative Phytochemical Investigation of the Greek Members of the Genus Mill., with Emphasis on the Local Endemic Boiss and Orph.

The members of the genus Mill. are notable for producing a diverse range of structurally intricate secondary metabolites, being the focus of current phytochemical research. Their importance is recognized as several species hold significant ethnopharmacological value, being traditionally used to address ailments in human systems, such as respiratory, gastrointestinal, and urinary conditions, among others.

Virulence of Five Root-Knot Nematodes ( spp.) on Nine Industrial Hemp ( L.) Varieties and Nematicidal Potential of Hemp Seed Extracts Against .

The most common and damaging plant parasitic nematodes are root-knot nematodes (RNK). Although hemp has been clearly infected by RNK, little information is available regarding the extent of the damage and losses caused. In addition, no information is available concerning hemp seed extracts' activity against RNK.

Application of Biochar-Immobilized for Enhancing Phosphorus Uptake and Growth in Rice.

Phosphorus (P) is an essential nutrient for rice growth, and the presence of phosphate-solubilizing bacteria (PSB) is an effective means to increase soil P content. However, the direct application of PSB may have minimal significance due to their low survival in soil. Biochar serves as a carrier that enhances microbial survival, and its porous structure and surface characteristics ensure the adsorption of .

In Vivo Assessment of Healing Potential of Ointments Containing Bee Products, Vegetal Extracts, and Polymers on Skin Lesions.

The present experiment aimed to formulate four ointments that included mixtures of plant extracts (, , , and ), apitherapy products (honey, propolis, and apilarnil) and natural polymers (collagen, chitosan, and the lyophilisate of egg white) in an ointment base. : In order to investigate the therapeutic properties of the ointments, experimental in vivo injury models (linear incision, circular excision, and thermal burns) were performed on laboratory animals, namely Wistar rats. The treatment was applied topically, once a day, for 21 days.

Trends of Soil and Solution Nutrient Sensing for Open Field and Hydroponic Cultivation in Facilitated Smart Agriculture.

Efficient management of soil nutrients is essential for optimizing crop production, ensuring sustainable agricultural practices, and addressing the challenges posed by population growth and environmental degradation. Smart agriculture, using advanced technologies, plays an important role in achieving these goals by enabling real-time monitoring and precision management of nutrients. In open-field soil cultivation, spatial variability in soil properties demands site-specific nutrient management and integration with variable-rate technology (VRT) to optimize fertilizer application, reduce nutrient losses, and enhance crop yields.

Investigating the Adsorption and Corrosion Protection Efficacy and Mechanism of Extract on Mild Steel in HCl Medium.

In recent years, the anti-corrosive properties of natural extracts as environmentally friendly inhibitors have gained considerable interest. This study evaluates the potential of ( L.) essential oil (), collected from Salé, Morocco, as a corrosion inhibitor for mild steel in 1 M HCl medium.

Biological and Health-Promoting Potential of Fruits from Three Cold-Hardy Actinidia Species.

Fruits are essential components of the human diet, valued for their diverse bioactive compounds with potential health-promoting properties. This study focuses on three cold-hardy species, namely , , and , examining their polyphenolic content, antioxidant/antiradical activities, scavenging capacity and effects on intestinal cell viability (Caco-2 and HT29-MTX). A comprehensive profile of their phenolic compounds was identified, in descending order of total polyphenol content: > > .